Acute Effect of Orange Juice Mixed With Oat β-Glucan on Bioavailability of Polyphenols in Healthy Individuals

NCT04867655 | Unknown

• 1 other identifier: Glasgow University
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

Brief summary Orange juice is the most widely consumed fruit juice, accounting for around a third of the total fruit juice market and is a rich source of vitamin C and bioactive compounds, predominantly flavonoids. Current research into the health effects of fruit juice consumption has presented some conflicting conclusions. Although potential health benefits have been attributed to the anti-inflammatory and anti-oxidant properties of the bioactive components in juice, other studies have suggested that the benefits of consuming orange are outweighed by the negative implications of the high sugar content leading to increases in blood glucose and insulin. At the same time it is well established that supplementation with a mean dose of 5g of β-Glucan, a soluble fibre derived from cereals such as oats or barley, significantly reduces insulin and glucose in healthy subjects and metabolic compromised individuals. Thus, the formulation of an OJ beverage with an added β-Glucan supplement may be a useful strategy to attenuate the detrimental impact of high sugar content. However, while delaying the absorption of glucose brings about favourable effects on post-prandial glycemia, dietary fibre may also reduce the bioavailability of some beneficial compounds, including polyphenols. So far, it remains unclear how addition of β-Glucan impacts bioavailability of orange juice flavanones. Thus, this study aims to determine how the bioavailability of orange juice polyphenols of healthy adults is affected mixing orange juice with 3 g and 6 g of oat β-Glucan.

Conditions

Healthy

Keywords

Flavanones; β-Glucan; Orange Juice; Biological Availability

Outcome Measures

Primary Outcomes (2)

• Plasma pharmacokinetics of naringenin, hesperetin, eriodictyol, isorhamnetin and phenolic acid metabolites including phenylpropionic acids, phenylacetic acid and benzoic acid derivatives

  Change in plasma concentrations collected at base line (0 hours) and 0.5,1, 2, 3,4,5,6,7,8, and 24 hours after ingestion of orange juice

  24 hours

• Urinary excretion of naringenin, hesperetin, eriodictyol, isorhamnetin and phenolic acid metabolites including phenylpropionic acids, phenylacetic acid and benzoic acid derivatives

  Change in concentrations in urinary fraction collected at base line (0 hours) and after ingestion of orange juice (0-5, 5-8, 8-10, 10-24 hours)

  24 hours

Secondary Outcomes (3)

• Body weight

  7 days

• Body fatness

  7 days

• Dietary Intake

  3 days and 24 hours

Study Arms (2)

Orange juice only

EXPERIMENTAL

Experimental test for 24 hours after consumption of orange juice only (Tropicana 'with bits').

Other: Orange Juice

Orange Juice mixed with either 6g or 3 g of β-Glucan

EXPERIMENTAL

Experimental test for 24 hours after consumption of orange juice (Tropicana 'with bits') with either 6g or 3 g of β-Glucan.

Other: Orange juice with either 6g or 3 g of β-Glucan

Interventions

Orange Juice OTHER

Approximately 500 ml of orange juice will be consumed and then blood and urine samples will be collected for 24 hours

Orange juice only

Orange juice with either 6g or 3 g of β-Glucan OTHER

Approximately 500 ml of orange juice mixed with either 6g or 3 g of β-Glucan will be consumed and then blood and urine samples will be collected for 24 hours

Orange Juice mixed with either 6g or 3 g of β-Glucan

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy
• BMI (20 kg/m2-35 kg/m2)
• non-smoker
• not taking any drug therapies
• normal dietary habits

You may not qualify if:

• history of gastrointestinal diseases
• following a special diet
• take vitamin supplements, prebiotics, probiotics
• vegetarian
• engaged in strenuous exercise training
• heavy alcohol consumer
• pregnant or breastfeeding (females)

Sponsors & Collaborators

• University of Glasgow: lead

Study Sites (1)

Human Nutrition, College of Medicine, Veterinary and Life Science

Glasgow, G31 2ER, United Kingdom

Location

Related Publications (1)

• Pereira-Caro G, Almutairi TM, Caceres-Jimenez S, Moreno-Rojas JM, Malkova D, Garcia AL, Crozier A. Bioavailability of orange juice (poly)phenols: beta-glucan-rich oat bran decreases urinary excretion of flavanone phase II metabolites and enhances excretion of microbiota-derived phenolic catabolites. Free Radic Biol Med. 2023 Apr;199:34-43. doi: 10.1016/j.freeradbiomed.2023.02.002. Epub 2023 Feb 9.

  PMID: 36764628 DERIVED

Study Officials

• Dale Malkova, PhD

  University of Glasgow

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Senior Lecturer

Study Record Dates

• First Submitted: April 27, 2021
• First Posted: April 30, 2021
• Study Start: July 10, 2019
• Primary Completion: May 4, 2022
• Study Completion: May 4, 2022
• Last Updated: November 2, 2021

Record last verified: 2021-10

Locations

GB (1)