Effects of a Single Bout of Exercise on Transcriptomics and Metabolomics in Adipose Tissue

NCT04865965 | Completed

• 1 other identifier: STUDY00000139
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to determine the effects of a single bout of exercise on changes in the genes and small molecules in blood and fat tissue, before and after a bout of exercise. This research may contribute to a better understanding of the beneficial effects of exercise on the body.

Conditions

Obesity; Metabolic Complication

Keywords

adipose tissue transcriptomics; adipose tissue metabolomics; peripheral blood mononuclear cell transcriptomics; obesity; exercise

Outcome Measures

Primary Outcomes (2)

• Transcriptome in white adipose tissue

  Gene transcription changes in RNA-based expression within subcutaneous adipose tissue

  Baseline; 2 hours

• Metabolome in fat

  Changes in concentration of metabolites within subcutaneous adipose tissue

  Baseline; 2 hours

Secondary Outcomes (1)

• Transcriptomic changes in peripheral blood mononuclear cell in people with obesity

  Baseline; 2 hours

Study Arms (2)

Acute Bout of Exercise

EXPERIMENTAL

Exercising for 60 minutes at 55-60% of VO₂ peak

Procedure: Acute Bout of Exercise

No Exercise

NO INTERVENTION

No Exercise Session

Interventions

Acute Bout of Exercise PROCEDURE

Exercising for 60 minutes at 55-60% of VO₂ peak

Acute Bout of Exercise

Eligibility Criteria

• Age: 20 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Age 20-60 years
• Body mass index (BMI) ≥30.0 ± 0.1 and ≤40.0 ± 0.1 kg/m2
• HbA1c ≤ 5.7 ± 0.1%
• No history of diabetes (type 1 and type 2)
• In good general health with no conditions that could influence the outcome of the trial, and in the judgment of the investigator is a good candidate for the study based on review of available medical history, physical examination and clinical laboratory evaluations
• Willing to adhere to the protocol requirements for the duration of the study

You may not qualify if:

• Exercises frequently i.e. ≥ 150 minutes per week of moderate intensity or ≥ 75 min high intensity.
• Blood glucose rises ≥140mg/dl at 2 hours after OGTT on Visit 1
• Use of weight loss medications (i.e. GLP-1 agonists)
• Use of any medications related to diabetes
• Use of beta-blockers
• Use of anticoagulation and aspirin
• Use of agents that affect hepatic glucose production such as beta adrenergic agonists, xanthine derivatives
• Currently pregnant or breastfeeding
• Current smoker
• Heart or lung disease
• Acute infection, including COVID-19 (current or recent infection of COVID-19 in the previous 3 months)
• Any hospitalization due to COVID-19.
• Severe hypertension (systolic \>160 mmHg or diastolic \>90 mmHg)
• Inability to exercise for any reason
• Any known contraindication to exercise testing based on current ACSM guidelines (5)

Sponsors & Collaborators

• Joslin Diabetes Center: lead
• Massachusetts Institute of Technology: collaborator

Study Sites (1)

Joslin Diabetes Center

Boston, Massachusetts, 02215, United States

Location

MeSH Terms

Conditions

Obesity; Motor Activity

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Behavior

Study Officials

• Roeland JW Middelbeek, MD

  Joslin Diabetes Center

  PRINCIPAL INVESTIGATOR

• Laurie Goodyear, PhD

  Joslin Diabetes Center

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 13, 2021
• First Posted: April 29, 2021
• Study Start: May 27, 2021
• Primary Completion: May 23, 2024
• Study Completion: May 23, 2024
• Last Updated: February 24, 2025

Record last verified: 2025-02

Locations

US (1)