Effect of Hypoxia Exercise on Erythrocyte Aggregability

NCT04805359 | Completed

• 1 other identifier: 200903510B0
• Study Type: interventional
• Target: 60
• Locations: 0 countries
• Sites: N/A

Brief Summary

Pathological erythrocyte aggregation reduces capillary perfusion and oxygen transfer to tissue. The aggregation is determined by opposing forces, the repulsive force between cells, cell-to-cell adhesion induced by plasma fibrinogen, and the disaggregating shear force generated by blood flow. The investigators investigate how hypoxic exercise affects intrinsic/extrinsic factors of aggregation. Sixty sedentary males were randomly assigned into either hypoxic (HE; FiO2=15%, n=20) or normoxic (NE; FiO2=21%, n=20) training groups for 30 min·d-1, 5 d·wk-1 for 6 weeks at 60% of maximum work rate or to a control group (CTL; n=20). A hypoxia exercise test (HET, FiO2=12%) was performed before and after the intervention. The erythrocyte aggregation, binding affinity of fibrinogen and membrane biomarkers were determined by an ektacytometry and flow cytometry, respectively.

Conditions

Hypoxia; Training

Keywords

Erythrocyte; Aggregation

Outcome Measures

Primary Outcomes (1)

• Total Erythrocyte Aggregation

  The total erythrocyte aggregation was determined by using Microfluidic Ektacytometer (Rheoscan-AnD 300, RheoMeditech, Seoul, Korea).

  8 weeks

Secondary Outcomes (7)

• Cardiopulmonary capacity

  8 weeks

• The affinity between erythrocyte and fibrinogen

  8 weeks

• Senescence-Related Molecules on Erythrocyte

  8 weeks

• Adhesion-Related Molecules on Erythrocyte

  8 weeks

• The influences of fibrinogen to erythrocytes

  8 weeks

Study Arms (3)

Normoxic exercise (NE)

EXPERIMENTAL

The subjects were trained on a bicycle ergometer at 60% of maximal work-rate (60%Wmax) under 21%O2 in air (NE) for 30 minutes per day, 5 days per week for 6 weeks

Behavioral: Normoxic exercise

Hypoxic exercise (HE)

EXPERIMENTAL

The subjects were trained on a bicycle ergometer at 60% of maximal work-rate (60%Wmax) under 15%O2 in air (HE) for 30 minutes per day, 5 days per week for 6 weeks

Behavioral: Hypoxic exercise

Normoxic control

NO INTERVENTION

Without any exercise training

Interventions

Normoxic exercise BEHAVIORAL

Trained on a bicycle ergometer at 60% of maximal work-rate (60%Wmax)for 30 minutes per day, 5 days per week for 6 weeks

Normoxic exercise (NE)

Hypoxic exercise BEHAVIORAL

Trained on a bicycle ergometer at 60% of maximal work-rate (60%Wmax)for 30 minutes per day, 5 days per week for 6 weeks

Hypoxic exercise (HE)

Eligibility Criteria

• Age: 20 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Having a sedentary lifestyle (without regular exercise, exercise frequency ≤ once weekly, duration \< 20 min).

You may not qualify if:

• Exposed to high altitudes (\> 3000 m) for at least 1 year.
• Smoker
• Taking medications or vitamins
• Having any cardiopulmonary/hematological risk.

Sponsors & Collaborators

• Chang Gung Memorial Hospital: lead

MeSH Terms

Conditions

Hypoxia

Condition Hierarchy (Ancestors)

Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Officials

• Jong-Shyan Wang, PhD

  Chang Gung Memorial Hospital

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: February 25, 2021
• First Posted: March 18, 2021
• Study Start: July 5, 2010
• Primary Completion: June 30, 2011
• Study Completion: June 30, 2011
• Last Updated: March 18, 2021

Record last verified: 2021-03

Data Sharing

• IPD Sharing: Will not share