GCF Levels of IL-1Beta, MIP-1 Alfa and G-CSF in Gingivitis and Stage I-II-III-IV Periodontitis Patients

NCT04629313 | Completed

• 1 other identifier: UsakU6
• Study Type: observational
• Target: 120
• Locations: 1 country
• Sites: 1

Brief Summary

Periodontal diseases are chronic diseases that occur as a result of a violation of the balance between microbial dental plaque and the host response. Gingivitis is a disease characterized by inflammation of the gums that occurs in one or more areas without loss of attachments.1 in periodontitis, an inflammatory event that begins in the gum along with gingivitis spreads to the periodontal ligament, alveolar bone and soft tissues that support the tooth, causing the destruction of these structures.2 Cytokines are low molecular weight proteins that participate in the initial and active stages of inflammation and immunity. In periodontal disease pathogenesis, cytokine response has been reported to play a very critical role in determining disease progression.3 IL-1beta, MIP-1alfa and G-CSF are key cytokines in chronic inflammatory diseases and have the potential to initiate bone loss and tissue destruction seen in periodontal disease.4the purpose of this study; it is to determine the degree of inflammation and periodontal destruction by determining the levels of IL-1beta, MIP-1alfa, G-CSF cytokines in the gum groove fluid of periodontal healthy and diseased individuals.

Conditions

Periodontitis

Keywords

periodontal disease; periodontitis; cytokines

Outcome Measures

Primary Outcomes (1)

• Measurement of IL-1Beta, G-CSF, MIP-1alfa levels in GCF

  Measurement of GCF samples

  GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement. GCF was obtained using strips of filter paper† from the buccal part of an interproximal region.

Study Arms (2)

periodontal disease group

Gingivitis: BOP score of 10% or greater and PD≤3mm with no attachment or radiographic bone loss. SI Periodontitis: interdental AL of 1-2 mm, PD≤4 mm and no tooth loss SII Periodontitis: interdental AL of 3-4 mm, PD≤5 mm and no tooth loss SIII Periodontitis: interdental AL ≥5 mm, PD≥6 mm SIV Periodontitis: interdental AL ≥5 mm teeth, radiographic bone lose extending to middle or apical third of the root,

periodontal healthy group

consisted of individuals with clinically healthy gingiva

Eligibility Criteria

• Age: 20 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

individuals who have periodontal disease will be selected

You may qualify if:

• Clinical diagnosis of periodontal diseases
• Individuals must have 16 permanent teeth in their mouth

You may not qualify if:

• Diabetes
• İmmunological diseases

Sponsors & Collaborators

• Uşak University: lead

Study Sites (1)

University of Usak

Uşak, 64200, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

gingival crevicular fluid

MeSH Terms

Conditions

Periodontitis; Periodontal Diseases

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases

Study Officials

• ahu dikilitaş

  University of Usak

  PRINCIPAL INVESTIGATOR

• fatih karaaslan

  University of Usak

  STUDY CHAIR

• umut yiğit

  University of Usak

  STUDY CHAIR

• esra özge aydın

  University of Usak

  STUDY CHAIR

• Abdullah seçkin ertuğrul

  University of Usak

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal investigator

Study Record Dates

• First Submitted: November 9, 2020
• First Posted: November 16, 2020
• Study Start: July 1, 2020
• Primary Completion: October 30, 2020
• Study Completion: October 30, 2020
• Last Updated: October 5, 2021

Record last verified: 2021-09

Locations

TU (1)