NCT04432597

Brief Summary

Background: For some cancers associated with human papillomavirus (HPV), standard treatments are not helpful. Researchers want to see if a vaccine for HPV combined with a drug called M7824 (MSB0011359C) has a better effect on these cancers than when they work alone. Objective: To find a safe dose of HPV vaccine alone or combined with M7824. Also, to test if either HPV vaccine alone or combined with M7824 causes a better immune response. Eligibility: People ages 18 and older with locally advanced or metastatic HPV associated cancer (Phase I) or stage II or III p16-positive oropharyngeal cancer (Phase II) Design: Participants will be screened with: Medical history Physical exam Blood, urine, and heart tests Possible photos of skin lesions Computed tomography (CT), magnetic resonance imaging (MRI), or nuclear bone scan: Participants will lie in a machine that takes pictures of the body. For the CT scan, they may have a contrast agent injected into a vein. Participants may have up to 2 tumor biopsies. For participants in Phase II, this may be performed with a thin tube placed through the nose into the airway. Participants will receive the HPV vaccine alone or with M7824. For participants on the Phase II, they will receive two doses of HPV vaccine under the skin either alone or with M7824 as an infusion spaced two weeks apart. This will be done prior to their planned chemoradiation or surgery. For participants on the Phase I, they will get the HPV vaccine injected under the skin 2 to 3 times in the first month. Then they will have a booster every 4 weeks. They will receive M7824 as an infusion into a vein every 2 weeks. Treatment will last up to 1 year. After they stop treatment, participants will have a visit within 4 weeks. They will then be contacted for long-term follow-up every year, for the rest of their lives. ...

Trial Health

55
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
39

participants targeted

Target at P50-P75 for phase_1

Timeline
Completed

Started Aug 2020

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 13, 2020

Completed
3 days until next milestone

First Posted

Study publicly available on registry

June 16, 2020

Completed
2 months until next milestone

Study Start

First participant enrolled

August 11, 2020

Completed
2.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 22, 2022

Completed
3.4 years until next milestone

Study Completion

Last participant's last visit for all outcomes

March 31, 2026

Completed
Last Updated

February 17, 2026

Status Verified

February 1, 2026

Enrollment Period

2.3 years

First QC Date

June 13, 2020

Last Update Submit

February 12, 2026

Conditions

Keywords

HPV Associated MalignancyImmunotherapyHPV VaccinePRGN-2009M7824

Outcome Measures

Primary Outcomes (2)

  • Safety and recommended phase II dose of PRGN-2009

    Phase I: In participants with recurrent/metastatic HPV positive cancer - To determine the safety and recommended phase II dose (RP2D) of PRGN-2009 (HPV vaccine) alone or in combination with M7824 administered at RP2D of 1200 mg q2w.

    one year

  • Level increase in CD3+ tumor infiltrating T cells post-treatment compared to pre-treatment

    Phase II: In participants with newly diagnosed stage I (T1,T2 N1)/II/III p16-positive oropharyngeal cancer - To determine if HPV vaccine alone (Arm 2A) is able to result in a equal to or greater than 2-fold increase in CD3+ tumor infiltrating T cells post treatment compared with pre-treatment in p16-positive oropharyngeal cancer.

    one year

Secondary Outcomes (8)

  • ratio of participants that are hospitalized because of adverse events attributed to disease progression

    study end

  • 3-year overall and relapse-free survival rate for PRGN-2009 alone

    study end

  • overall survival (OS)

    study end

  • progression-free survival time (PFS)

    study end

  • duration of response

    study end

  • +3 more secondary outcomes

Study Arms (4)

1/Arm 1A

EXPERIMENTAL

Human Papillomavirus Vaccine (HPV) vaccine at 1x10(11) Viral Particles (VP) Dose Level 1 (DL1) and at 5x10(11) VP Dose Level 2 (DL2)

Biological: PRGN-2009Diagnostic Test: EKGProcedure: BiopsyDiagnostic Test: CT ScanDiagnostic Test: MRIDrug: Brain CTDrug: Brain MRIDrug: DexamethasoneDrug: EpinephrineDrug: DiphenhydramineDrug: Ibuprofen

2/Arm 1B

EXPERIMENTAL

Human Papillomavirus Vaccine (HPV) vaccine at recommended phase 2 dose (RP2D) plus M7824 at 1200 mg.

Biological: PRGN-2009Biological: M7824Diagnostic Test: EKGProcedure: BiopsyDiagnostic Test: CT ScanDiagnostic Test: MRIDrug: Brain CTDrug: Brain MRIDrug: DexamethasoneDrug: EpinephrineDrug: DiphenhydramineDrug: Ibuprofen

3/Arm 2A

EXPERIMENTAL

Human Papillomavirus Vaccine (HPV) vaccine at recommended phase 2 dose (RP2D) given as neoadjuvant or induction therapy.

Biological: PRGN-2009Diagnostic Test: EKGProcedure: BiopsyDiagnostic Test: CT ScanDiagnostic Test: MRIDrug: Brain CTDrug: Brain MRIDrug: DexamethasoneDrug: EpinephrineDrug: DiphenhydramineDrug: Ibuprofen

4/Arm 2B

EXPERIMENTAL

Human Papillomavirus Vaccine (HPV) vaccine at recommended phase 2 dose (RP2D) plus M7824 at 1200 mg given as neoadjuvant or induction therapy.

Biological: PRGN-2009Biological: M7824Diagnostic Test: EKGProcedure: BiopsyDiagnostic Test: CT ScanDiagnostic Test: MRIDrug: Brain CTDrug: Brain MRIDrug: DexamethasoneDrug: EpinephrineDrug: DiphenhydramineDrug: Ibuprofen

Interventions

PRGN-2009BIOLOGICAL

On the phase I portion of the protocol PRGN-2009 will be administered on Day (D)1, D15, D29 followed by booster vaccines every 4 weeks for up to a year. The dose level given as booster will be the same dose participants will be receiving for D1, D15 and D29. On the phase II portion of the protocol PRGN-2009 will be administered on just D1 and D15 for Cohort 1/Arm 1A, Cohort 2/Arm 1B, Cohort 3/Arm 2A, and Cohort 4/Arm2B.

1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B
M7824BIOLOGICAL

Subjects enrolled to Cohort 2/Arm 1B will receive M7824 (MSB0011359C) via intravenous (IV) infusion over 1 hour (-10 minutes / +20 minutes, that is, over 50 to 80 minutes) once every 2 weeks. M7824 will be administered as a "flat" dose of 1,200 mg independent of body weight. M7824 is administered as an intravenous infusion with a mandatory 0.2 micron in-line filter.

Also known as: MSB0011359C
2/Arm 1B4/Arm 2B
EKGDIAGNOSTIC_TEST

Screening, end of treatment and follow-up.

Also known as: Electrocardiogram
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B
BiopsyPROCEDURE

For immune analysis: Baseline Day 1 and odd numbered weeks (week(W) 1, W3, W5, W7 onwards.

Also known as: Bx
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B
CT ScanDIAGNOSTIC_TEST

CT scan chest, abdomen, pelvis, neck, and/or skull as clinically indicated. Tumor evaluation at screening, baseline Day 1, odd numbered weeks (week(W) 1, W3, W5, W7 onwards. Long term follow-up.

Also known as: Computed tomography
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B
MRIDIAGNOSTIC_TEST

Tumor evaluation at screening, baseline Day 1, odd numbered weeks (week(W) 1, W3, W5, W7 onwards. Long term follow-up.

Also known as: Magnetic resonance imaging
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

Screening. As clinically indicated in participants with known central nervous system (CNS) disease.

Also known as: Brain computed tomography
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

Screening. As clinically indicated in participants with known central nervous system (CNS) disease.

Also known as: Brain magnetic resonance imaging
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

Dexamethasone 10mg for hypersensitivity reactions.

Also known as: Decadron, Ozurdex, Dexycu
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

1:1,000 dilution for hypersensitivity reactions.

Also known as: Adrenaline
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

Antihistamines (e.g., Diphenhydramine) given intravenously for allergy.

Also known as: Benadryl
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

For flu-like symptoms. 400mg or comparable Nonsteroidal anti-inflammatory drugs (NSAID) dose, may be administered 2 hours before and 8 hours after the start of each intravenous infusion.

Also known as: Advil, Motrin IB, Midol IB
1/Arm 1A2/Arm 1B3/Arm 2A4/Arm 2B

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Subjects with cytologically or histologically confirmed locally advanced not amenable to potentially curative local therapies or metastatic human papillomavirus (HPV) associated malignancies (Phase I only):
  • Cervical cancers;
  • p16+ Oropharyngeal cancers;
  • Anal cancers;
  • Vulvar, vaginal, penile, and squamous cell rectal cancers;
  • Other locally advanced or metastatic solid tumors (e.g., lung, esophagus) that are known HPV+.
  • Subjects with cytologically or histologically confirmed newly diagnosed stage II or III p16-positive oropharyngeal squamous cell carcinoma planned for definitive therapy or with newly diagnosed stage II or III or IVA or IVB HPV-positive sinonasal squamous carcinoma (HPV-SNSCC) eligible for primary surgery (Phase II only).
  • Subjects must have measurable disease, per Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 (Phase 1 only).
  • Phase I only: Participants must have received one prior line of systemic chemotherapy in the recurrent/metastatic setting as well as checkpoint blockade therapy in tumors with Food and Drug Administration (FDA) approval (head and neck squamous cell cancer and programmed death-ligand 1 (PDL1+) cervical cancer). Exceptions to this include participants not eligible to receive standard therapy.
  • Men or Women; Age \>=18 years.
  • Eastern Cooperative Oncology Group (ECOG) performance status =\< 2
  • Adequate hematologic function at screening, as follows:
  • Absolute neutrophil count (ANC) \>=1 x 109/L;
  • Hemoglobin \>= 9 g/dL;
  • Platelets \>= 75,000/microliter.
  • +6 more criteria

You may not qualify if:

  • Participants with prior investigational drug, live vaccine, chemotherapy, immunotherapy or any prior radiotherapy (except for palliative bone directed therapy) within the past 28 days prior to the first drug administration except if the investigator has assessed that all residual treatment-related toxicities have resolved or are minimal and feel the participant is otherwise suitable for enrollment. Participants may continue adjuvant hormonal therapy in the setting of a definitively treated cancer (e.g., breast).
  • Major surgery within 28 days prior to the first drug administration (minimally invasive procedures such as diagnostic biopsies are permitted).
  • Known active brain or central nervous system metastasis (less than a month out from definitive radiotherapy or surgery), seizures requiring anticonvulsant treatment (\<3 months) or clinically significant cerebrovascular accident (\<3 months). In order to be eligible participants must have repeated central nervous system (CNS) imaging at least a month after definitive treatment showing stable CNS disease. Participants with evidence of intra-tumoral or peritumoral hemorrhage on baseline imaging are also excluded unless the hemorrhage is grade =\< 1 and has been shown to be stable on two consecutive imaging scans.
  • Pregnant women are excluded from this study because M7824 and PRGN-2009 vaccine have not been tested in pregnant women and there is potential for teratogenic or abortifacient effects. Because there is an unknown but potential risk for adverse events in nursing infants secondary to treatment of the mother with these immunotherapies, breastfeeding should be discontinued if the mother is treated on this protocol.
  • Only for Phase I, Arm 1B: Active autoimmune disease that might deteriorate when receiving an immunostimulatory agent with exception of:
  • Diabetes type I, eczema, vitiligo, alopecia, psoriasis, hypo- or hyperthyroid disease or other mild autoimmune disorders not requiring immunosuppressive treatment;
  • Administration of steroids for other conditions through a route known to result in a minimal systemic exposure (topical, intranasal, intro-ocular, or inhalation) is acceptable;
  • Subjects on systemic intravenous or oral corticosteroid therapy with the exception of
  • physiologic doses of corticosteroids (=\< the equivalent of prednisone 10 mg/day) or other immunosuppressors such as azathioprine or cyclosporin A are excluded on the basis of potential immune suppression. For these subjects these excluded treatments must be discontinued at least 1 weeks prior to enrollment for recent short course use (=\< 14 days) or discontinued at least 4 weeks prior to enrollment for long term use (\> 14 days). In addition, the use of corticosteroids as premedication for contrast-enhanced studies is allowed prior to enrollment and on study.
  • Only for Phase I: Subjects with a history of serious intercurrent chronic or acute illness, such as cardiac or pulmonary disease, hepatic disease, bleeding diathesis or recent (within 3 months) clinically significant bleeding events, known left ventricular ejection fraction \<50% (confirmation of ejection fraction (EF) \> 50% is not required for eligibility), history of myocarditis, or recent myocardial infarction (within 6 months), or other illness considered by the Investigator as high risk for M7824 drug treatment.
  • Only for Phase I: Subjects refusing to accept blood products as medically indicated.
  • History of second malignancy within 3 years of enrollment except for the following: adequately treated localized skin cancer, cervical carcinoma in situ, superficial bladder cancer, other localized malignancy which has been adequately treated or malignancy which does not require active systemic treatment (e.g., low risk chronic lymphocytic leukemia (CLL)). For participants enrolled on the phase I portion of the protocol a second HPV driven malignancy is allowed.
  • Only for Phase I, Arm 1B: Subjects with a known severe hypersensitivity reaction to monoclonal antibodies or its excipients (grade \>/= 3 National Cancer Institute (NCI)-Common Terminology Criteria for Adverse Events (CTCAE) v5) will be evaluated by the allergy/immunology team prior to enrollment.
  • Prior allogenic tissue/solid organ transplant.
  • For participants who may receive M7824: previous life-threatening side effects resulting from prior checkpoint inhibitor therapy.
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

National Institutes of Health Clinical Center

Bethesda, Maryland, 20892, United States

Location

Related Publications (1)

  • Floudas CS, Goswami M, Donahue RN, Strauss J, Pastor DM, Redman JM, Brownell I, Turkbey EB, Steinberg SM, Cordes LM, Marte JL, Khan MH, McMahon S, Lamping E, Manu M, Manukyan M, Brough DE, Lankford A, Jochems C, Schlom J, Gulley JL. PRGN-2009 and bintrafusp alfa for patients with advanced or metastatic human papillomavirus-associated cancer. Cancer Immunol Immunother. 2025 Mar 21;74(5):155. doi: 10.1007/s00262-025-04009-z.

Related Links

MeSH Terms

Conditions

Rectal NeoplasmsAnus NeoplasmsOropharyngeal NeoplasmsUterine Cervical Neoplasms

Interventions

ElectrocardiographyBiopsyTomography, X-Ray ComputedMagnetic Resonance ImagingDexamethasoneCalcium DobesilateEpinephrineDiphenhydramineIbuprofen

Condition Hierarchy (Ancestors)

Colorectal NeoplasmsIntestinal NeoplasmsGastrointestinal NeoplasmsDigestive System NeoplasmsNeoplasms by SiteNeoplasmsDigestive System DiseasesGastrointestinal DiseasesIntestinal DiseasesRectal DiseasesAnus DiseasesPharyngeal NeoplasmsOtorhinolaryngologic NeoplasmsHead and Neck NeoplasmsPharyngeal DiseasesStomatognathic DiseasesOtorhinolaryngologic DiseasesUterine NeoplasmsGenital Neoplasms, FemaleUrogenital NeoplasmsUterine Cervical DiseasesUterine DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital Diseases

Intervention Hierarchy (Ancestors)

Heart Function TestsDiagnostic Techniques, CardiovascularDiagnostic Techniques and ProceduresDiagnosisElectrodiagnosisCytodiagnosisCytological TechniquesClinical Laboratory TechniquesSpecimen HandlingDiagnostic Techniques, SurgicalSurgical Procedures, OperativeInvestigative TechniquesImage Interpretation, Computer-AssistedDiagnostic ImagingRadiographic Image EnhancementImage EnhancementPhotographyRadiographyTomography, X-RayTomographyPregnadienetriolsPregnadienesPregnanesSteroidsFused-Ring CompoundsPolycyclic CompoundsSteroids, FluorinatedBenzenesulfonatesBenzene DerivativesHydrocarbons, AromaticHydrocarbons, CyclicHydrocarbonsOrganic ChemicalsArylsulfonatesArylsulfonic AcidsSulfonic AcidsSulfur AcidsSulfur CompoundsEthanolaminesAmino AlcoholsAlcoholsAminesBiogenic MonoaminesBiogenic AminesCatecholaminesCatecholsPhenolsEthylaminesBenzhydryl CompoundsPhenylpropionatesAcids, CarbocyclicCarboxylic Acids

Study Officials

  • Charalampos Floudas, M.D.

    National Cancer Institute (NCI)

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SEQUENTIAL
Sponsor Type
NIH
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

June 13, 2020

First Posted

June 16, 2020

Study Start

August 11, 2020

Primary Completion

November 22, 2022

Study Completion

March 31, 2026

Last Updated

February 17, 2026

Record last verified: 2026-02

Data Sharing

IPD Sharing
Will share

All individual participant data (IPD) recorded in the medical record will be shared with intramural investigators upon request. In addition, all large-scale genomic sequencing data will be shared with subscribers to the database of Genotypes and Phenotypes (dbGaP).

Shared Documents
STUDY PROTOCOL, SAP, ICF
Time Frame
Clinical data available during the study and indefinitely. Genomic data are available once genomic data are uploaded per protocol Genomic Data Sharing (GDS) plan for as long as database is active.
Access Criteria
Clinical data will be made available via subscription to Biomedical Translational Research Information System (BTRIS) and with the permission of the study principal investigator (PI). Genomic data are made available via the database of Genotypes and Phenotypes (dbGaP) through requests to the data custodians.

Locations