GCF IL-17 Levels and Oxidative Status of Smoking and Non-smoking Individuals With Periodontitis

NCT04115176 | Completed

• 1 other identifier: PYO.DIS.1901.17.002
• Study Type: observational
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

This study was performed to evaluate the effects of both periodontal treatment and smoking on the gingival crevicular fluid(GCF) levels of interleukin (IL)-17A, IL-17E, Total oxidative stress(TOS) and total antioxidant capasity(TAOC) in smoking and non-smoking patients with severe chronic periodontitis. Systemically healthy 15 smoker,15 non-smoker with chronic periodontitis were attended for the study. Periodontal status were determined by recording periodontal clinical measurements. GCF samples were collected at the baseline, at the end of initial periodontal treatment and 4 weeks after periodontal flap surgery. The data were tested statistically by the Student t and paired sample t tests.

Conditions

Chronic Periodontitis; Smoking, Cigarette

Keywords

chronic periodontitis; smoking,; periodontal treatment,; IL-17,; oxidative stress

Outcome Measures

Primary Outcomes (4)

• GCF IL-17A VOLUME

  IL-17A is a cytokine present in gingival crevicular fluid. Its volume calculated as microliter and changes due to inflammation caused by periodontal disease.

  GCF sampling was performed prior to periodontal treatment , 4 weeks after completion of initial periodontal treatment, and 4 weeks after periodontal flap surgery .

• GCF IL-17E VOLUME

  IL-17E is a cytokine present in gingival crevicular fluid. Its volume calculated as microliter and changes due to inflammation caused by periodontal disease.

  GCF sampling was performed prior to periodontal treatment , 4 weeks after completion of initial periodontal treatment, and 4 weeks after periodontal flap surgery .

• GCF TOTAL OXIDATIVE STRESS (TOS) LEVEL

  TOS is the measurement of oxidative stress amount and given as mmol/L unit. Its amount changes due to inflammation caused by periodontal disease.

  GCF sampling was performed prior to periodontal treatment , 4 weeks after completion of initial periodontal treatment, and 4 weeks after periodontal flap surgery .

• GCF TOTAL ANTIOXIDANT CAPASITY (TAOC)

  TAOC is the antioxidant amount measured and given as μmol H2O2 Eq/L unit. Its amount changes due to inflammation caused by periodontal disease.

  GCF sampling was performed prior to periodontal treatment , 4 weeks after completion of initial periodontal treatment, and 4 weeks after periodontal flap surgery .

Study Arms (2)

smokers + periodontitis

Individuals clinically diagnosed with chronic periodontitis that smoke recruited for this group.

nonsmoker + periodontitis

Individuals clinically diagnosed with chronic periodontitis that don't smoke recruited for this group.

Eligibility Criteria

• Age: 35 Years - 60 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Patients with chronic periodontitis (with an age range of 35 to 60 years) seeking dental treatment at the Periodontology Clinic, School of Dentistry, Ondokuz Mayis University, were included in the present study.

You may qualify if:

• individuals aged ˃35 years and having ≥16 teeth diagnosed as chronic periodontitis
• not received any periodontal therapy within the previous 6 months;
• not received antibiotic/anti-inflammatory drug treatment within the previous 6 months.

You may not qualify if:

• receiving medical treatment for cancer, rheumatoid arthritis, diabetes mellitus, cardiovascular disease, or any other systemic disease;
• being pregnant or being in the period of menopause or lactation;
• receiving any drug therapy that might affect the clinical characteristics of periodontitis;
• usage of systemic antimicrobials during the 6 weeks prior to data collection

Sponsors & Collaborators

• Ondokuz Mayıs University: lead

Study Sites (1)

Müge Lütfioğlu

Samsun, Atakum, 55039, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Chronic Periodontitis; Cigarette Smoking; Smoking

Condition Hierarchy (Ancestors)

Periodontitis; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Tobacco Smoking; Behavior; Tobacco Use

Study Officials

• MÜGE LÜTFİOĞLU

  assoc. prof.

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: October 2, 2019
• First Posted: October 3, 2019
• Study Start: October 10, 2017
• Primary Completion: July 10, 2019
• Study Completion: July 10, 2019
• Last Updated: October 4, 2019

Record last verified: 2019-10

Locations

TU (1)