Skeletal Muscle Protein Synthesis: Traditional and Cluster Sets

NCT04028726 | Completed DMC

• 1 other identifier: 16987
• Study Type: interventional
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

Traditionally, when performing resistance exercise, repetitions are performed in a continuous manner resulting in a decrease in velocity, and thus power output. "Cluster" set is an alternative to counter the reduction in velocity and power output. Cluster sets incorporate a brief rest (15-30 seconds) between individual repetitions (inter-repetition rest) or group of repetitions (intra-set rest) within a set of resistive exercise. In contrast to traditional set configurations, which result in an almost linear decrease in force, velocity, and power -cluster sets maintain and/or attenuate the loss in power, primarily due to higher velocities. The purpose of this research is to compare the muscle protein synthesis response to acute bouts of cluster and traditional set configurations. Investigators will assess muscle protein synthesis rates before and after acute resistance exercise in 14 resistance-trained males and females (Age: 20-35; back squat 1.5 x body weight; 3 years of resistance training experience). The volunteers are required to be removed (at least six weeks) from all ergogenic levels of nutritional supplements that have previously been shown to affect protein synthesis. Blood and muscle biopsies will be collected as a part of testing. Further, subjects will be required to perform moderate-to-high intensity resistance exercise.

Conditions

Resistance Exercise

Outcome Measures

Primary Outcomes (1)

• Fractional Synthetic Rate of Myofibrillar Proteins

  Myofibrillar muscle protein synthesis rates will be assessed during the 2hr Postabsorptive period (e.g. non-fed state) and the 5hr postprandial period (e.g. fed state) for both experimental interventions (e.g. leucine and leucine peptide). This will allow us to assess the change in fractional synthetic rate from the Postabsorptive period to the postprandial period.

  Postabsorptive for 2 hours, Postprandial for 5 hours

Study Arms (2)

Cluster set Resistance Training Protocol

ACTIVE COMPARATOR

Cluster set configuration is the new training approach that it is being tested.

Other: Back-squat exercise

Traditional Resistance Training Protocol

SHAM COMPARATOR

Traditional is commonly used in training sessions.

Other: Back-squat exercise

Interventions

Back-squat exercise OTHER

Participants begin with a 5 min dynamic warm-up. Then, they will perform two sets of five repetitions at 40-60% of predetermined 1-repetition maximum (1-RM). The clustered bout will be 4 sets of 2 clusters of 5 repetitions with a 30-second intra-set rest between clusters, and 90-second inter-set rest at a load 70% of 1-RM. The traditional bout will be 4 sets of 10 repetitions with a 120-second inter-set rest at a load 70% of 1-RM.

Cluster set Resistance Training Protocol; Traditional Resistance Training Protocol

Eligibility Criteria

• Age: 20 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Resistance-trained adults
• Back squat 1.5x body weight
• years resistance-training experience
• Aged between 20-35 years

You may not qualify if:

• Tobacco use (previous 6 months)
• Alcohol consumption \>10 drinks per week
• Metabolic disorders, diabetes, thyroid disease, hypogonadism
• Heart disease, arrhythmias, asthma
• History of uncontrolled hypertension
• Orthopaedic injury/surgery within the last year
• Hepatorenal, musculoskeletal, autoimmune, neuromuscular, or neurologic disease
• Consumed ergogenic levels of nutritional supplements that may affect muscle mass (i.e. creatine, HMB), insulin-like substances, or anabolic/catabolic pro-hormones (i.e. DHEA) within six weeks prior to the onset of the study
• Individuals consuming thyroid, androgenic, or other medications known to affect endocrine function
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti- inflammatories, or prescription strength acne medications)
• Pregnant

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead
• Texas Christian University: collaborator

Study Sites (1)

Freer Hall

Urbana, Illinois, 61801, United States

Location

Study Officials

• Nicholas Burd, PhD

  University of Illinois at Urbana-Champaign

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: August 2, 2018
• First Posted: July 23, 2019
• Study Start: August 25, 2018
• Primary Completion: October 25, 2018
• Study Completion: July 15, 2019
• Last Updated: July 24, 2019

Record last verified: 2019-07

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)