Effect of Aging & Obesity With Exercise Intervention

NCT03951857 | Completed DMC

• 1 other identifier: B-1610-365-001
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

Obesity leads to a vicious circle that increases intramuscular fat, insulin resistance, promotes muscular dysfunction resulting in increased muscle fat accumulation. The study of muscle function and intramyocellular lipids is insufficient for obesity. Particularly, the study of mechanisms in muscle function and intramyocellular lipids is few nationally and internationally.

Conditions

Obesity

Keywords

Aging; Obesity; Single muscle fiber contractile properties; Intramyocellular lipids; Resistance exercise

Outcome Measures

Primary Outcomes (7)

• Total droplet area

  Change from baseline total droplet area to 12 weeks after initial assessment. Total droplet area is determined by Oil-red-O staining. After staining, the total area (µm2) is analyzed to compare the change after intervention from baseline.

  Before intervention / after 12weeks intervention

• Number of lipid droplets

  Change from baseline number of lipid droplets to 12 weeks after initial assessment. Number of lipid droplets determined by Oil-red-O staining. After staining, number of lipid droplets is analyzed to compare the change after intervention from baseline.

  Before intervention / after 12weeks intervention

• Single muscle fiber cross-sectional area (CSA)

  Change from baseline CSA to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber CSA (µm2) is measured by 3 dimensional microscopy. after analysis, CSA is compared the change after intervention from baseline.

  Before intervention / after 12weeks intervention

• Single muscle fiber maximal force (Po)

  Change from baseline Po to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber Po (mN) is measured by slack test procedure. after measurement, Po is analyzed to compare the change after intervention from baseline.

  Before intervention / after 12weeks intervention

• Single muscle fiber specific force (SF)

  Change from baseline SF to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber SF (kN/m2) is Po (kN) normalized by cross-sectional area (m2). SF is analyzed to compare the change after intervention from baseline.

  Before intervention / after 12weeks intervention

• Single muscle fiber maximal shortening velocity (Vo)

  Change from baseline Vo to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber Vo (FL/s) is measured by slack test procedure. The time required to take up the imposed slack was measured from the onset of the length step to the beginning of the tension redevelopment . For each amplitude of length, the fiber was reextended while relaxed to minimize nonuniformity of sarcomere length. A straight line was presented to a plot of length vs. time, using least-squares regression, and the slope of the line divided by the segment length was recorded as Vo for that fiber. Vo is analyzed to compare the change after intervention from baseline.

  Before intervention / after 12weeks intervention

• Single muscle fiber MHC type distribution

  Change from baseline fiber type distribution to 12 weeks after initial assessment. The MHC composition of single fibers was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). MHC type distribution is analyzed to compare the change after intervention from baseline.

  Before intervention / after 12weeks intervention

Secondary Outcomes (2)

• Hand grip strength

  Before intervention / after 12weeks intervention

• Isokinetic knee extension strength

  Before intervention / after 12weeks intervention

Study Arms (4)

Young men

NO INTERVENTION

Range of age is 20-35 years, young men (BMI 19-23 Kg/m2) They did not suffer from musculoskeletal or metabolic diseases, and had not performed regular exercise within the previous 3 months.

Young women

NO INTERVENTION

Range of age is 20-35 years, young women (BMI 19-23 Kg/m2) They did not suffer from musculoskeletal or metabolic diseases, and had not performed regular exercise within the previous 3 months.

Elderly women (control)

EXPERIMENTAL

Range of age is 65-80 years, Elderly women (BMI 19-23 Kg/m2) They did not suffer from musculoskeletal or metabolic diseases, and had not performed regular exercise within the previous 3 months.

Other: Resistance exercise with a gym stick

Elderly obese women

EXPERIMENTAL

Range of age is 65-80 years,Elderly obese women (BMI ≥25 Kg/m2) They did not suffer from musculoskeletal or metabolic diseases, and had not performed regular exercise within the previous 3 months.

Other: Resistance exercise with a gym stick

Interventions

Resistance exercise with a gym stick OTHER

Exercise intervention: The resistance exercise intervention is performed with a gym stick for 3 times a week for 12 weeks. Exercise load is gradually increased by the number of times the band is wound and the speed of motion by dividing into three stages in 1-4 weeks (1st stage), 5-8 weeks (2nd stage) and 9-12 weeks (3rd stage).

Also known as: Exercise training

Elderly obese women; Elderly women (control)

Eligibility Criteria

• Age: 20 Years - 80 Years
• Gender Eligibility Details: This study is divided into two purposes. 1. To examine the distribution and contractile properties (CSA, Po, SF, Vo) of single muscle fiber sex/MHC type-related differences in youn men and women. 2. To examine effects of 12-week resistance exercise on single muscle fiber funtion and intramyocellular lipids in aging and obese elderly women. For the purposes of the first study, young men and women (age 20-35 yrs) should be participated in this study. aimed to examine the distribution and contractile properties (CSA, Po, SF, Vo) of single muscle fiber sex/MHC type-related differences, by extracting a single muscle fiber from the vastus lateralis in young men and women
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Young men: Age (20-35 yrs), BMI (19-23 Kg/m2)
• Young women: Age (20-35 yrs), BMI (19-23 Kg/m2)
• Elderly women: Age (65-80 yrs), BMI (19-23 Kg/m2)
• Elderly obese women: Age (65-80 yrs), BMI ( \>25 Kg/m2)
• Subjects who can agree voluntarily.

You may not qualify if:

• Acute coronary syndrome.
• Uncontrolled hypertension.
• Subjects who took drugs which can affects neuromuscular system.
• Severely impaired cognition
• Subjects who cannot agree voluntarily

Sponsors & Collaborators

• Seoul National University Bundang Hospital: lead
• National Research Foundation of Korea: collaborator

Study Sites (1)

Seoul National University Bundang Hospital

Seongnam-si, Gyeonggi-do, 463-707, South Korea

Location

Related Publications (18)

• Lexell J, Taylor CC, Sjostrom M. What is the cause of the ageing atrophy? Total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15- to 83-year-old men. J Neurol Sci. 1988 Apr;84(2-3):275-94. doi: 10.1016/0022-510x(88)90132-3.

  PMID: 3379447 BACKGROUND

• Doherty TJ, Vandervoort AA, Brown WF. Effects of ageing on the motor unit: a brief review. Can J Appl Physiol. 1993 Dec;18(4):331-58. doi: 10.1139/h93-029.

  PMID: 8275048 BACKGROUND

• Goodpaster BH, Park SW, Harris TB, Kritchevsky SB, Nevitt M, Schwartz AV, Simonsick EM, Tylavsky FA, Visser M, Newman AB. The loss of skeletal muscle strength, mass, and quality in older adults: the health, aging and body composition study. J Gerontol A Biol Sci Med Sci. 2006 Oct;61(10):1059-64. doi: 10.1093/gerona/61.10.1059.

  PMID: 17077199 BACKGROUND

• Hughes VA, Frontera WR, Wood M, Evans WJ, Dallal GE, Roubenoff R, Fiatarone Singh MA. Longitudinal muscle strength changes in older adults: influence of muscle mass, physical activity, and health. J Gerontol A Biol Sci Med Sci. 2001 May;56(5):B209-17. doi: 10.1093/gerona/56.5.b209.

  PMID: 11320101 BACKGROUND

• Goodpaster BH, Krishnaswami S, Resnick H, Kelley DE, Haggerty C, Harris TB, Schwartz AV, Kritchevsky S, Newman AB. Association between regional adipose tissue distribution and both type 2 diabetes and impaired glucose tolerance in elderly men and women. Diabetes Care. 2003 Feb;26(2):372-9. doi: 10.2337/diacare.26.2.372.

  PMID: 12547865 BACKGROUND

• Tuttle LJ, Sinacore DR, Mueller MJ. Intermuscular adipose tissue is muscle specific and associated with poor functional performance. J Aging Res. 2012;2012:172957. doi: 10.1155/2012/172957. Epub 2012 May 14.

  PMID: 22666591 BACKGROUND

• Yim JE, Heshka S, Albu J, Heymsfield S, Kuznia P, Harris T, Gallagher D. Intermuscular adipose tissue rivals visceral adipose tissue in independent associations with cardiovascular risk. Int J Obes (Lond). 2007 Sep;31(9):1400-5. doi: 10.1038/sj.ijo.0803621. Epub 2007 Apr 24.

  PMID: 17452994 BACKGROUND

• Visser M, Kritchevsky SB, Goodpaster BH, Newman AB, Nevitt M, Stamm E, Harris TB. Leg muscle mass and composition in relation to lower extremity performance in men and women aged 70 to 79: the health, aging and body composition study. J Am Geriatr Soc. 2002 May;50(5):897-904. doi: 10.1046/j.1532-5415.2002.50217.x.

  PMID: 12028178 BACKGROUND

• Koster A, Ding J, Stenholm S, Caserotti P, Houston DK, Nicklas BJ, You T, Lee JS, Visser M, Newman AB, Schwartz AV, Cauley JA, Tylavsky FA, Goodpaster BH, Kritchevsky SB, Harris TB; Health ABC study. Does the amount of fat mass predict age-related loss of lean mass, muscle strength, and muscle quality in older adults? J Gerontol A Biol Sci Med Sci. 2011 Aug;66(8):888-95. doi: 10.1093/gerona/glr070. Epub 2011 May 13.

  PMID: 21572082 BACKGROUND

• Addison O, Marcus RL, Lastayo PC, Ryan AS. Intermuscular fat: a review of the consequences and causes. Int J Endocrinol. 2014;2014:309570. doi: 10.1155/2014/309570. Epub 2014 Jan 8.

  PMID: 24527032 BACKGROUND

• Goodpaster BH, Carlson CL, Visser M, Kelley DE, Scherzinger A, Harris TB, Stamm E, Newman AB. Attenuation of skeletal muscle and strength in the elderly: The Health ABC Study. J Appl Physiol (1985). 2001 Jun;90(6):2157-65. doi: 10.1152/jappl.2001.90.6.2157.

  PMID: 11356778 BACKGROUND

• Yoshida Y, Marcus RL, Lastayo PC. Intramuscular adipose tissue and central activation in older adults. Muscle Nerve. 2012 Nov;46(5):813-6. doi: 10.1002/mus.23506.

  PMID: 23055318 BACKGROUND

• Visser M, Goodpaster BH, Kritchevsky SB, Newman AB, Nevitt M, Rubin SM, Simonsick EM, Harris TB. Muscle mass, muscle strength, and muscle fat infiltration as predictors of incident mobility limitations in well-functioning older persons. J Gerontol A Biol Sci Med Sci. 2005 Mar;60(3):324-33. doi: 10.1093/gerona/60.3.324.

  PMID: 15860469 BACKGROUND

• Ryan AS, Ortmeyer HK, Sorkin JD. Exercise with calorie restriction improves insulin sensitivity and glycogen synthase activity in obese postmenopausal women with impaired glucose tolerance. Am J Physiol Endocrinol Metab. 2012 Jan 1;302(1):E145-52. doi: 10.1152/ajpendo.00618.2010. Epub 2011 Oct 18.

  PMID: 22008454 BACKGROUND

• Santanasto AJ, Glynn NW, Newman MA, Taylor CA, Brooks MM, Goodpaster BH, Newman AB. Impact of weight loss on physical function with changes in strength, muscle mass, and muscle fat infiltration in overweight to moderately obese older adults: a randomized clinical trial. J Obes. 2011;2011:516576. doi: 10.1155/2011/516576. Epub 2010 Oct 10.

  PMID: 20953373 BACKGROUND

• Gorgey AS, Mather KJ, Cupp HR, Gater DR. Effects of resistance training on adiposity and metabolism after spinal cord injury. Med Sci Sports Exerc. 2012 Jan;44(1):165-74. doi: 10.1249/MSS.0b013e31822672aa.

  PMID: 21659900 BACKGROUND

• Menshikova EV, Ritov VB, Fairfull L, Ferrell RE, Kelley DE, Goodpaster BH. Effects of exercise on mitochondrial content and function in aging human skeletal muscle. J Gerontol A Biol Sci Med Sci. 2006 Jun;61(6):534-40. doi: 10.1093/gerona/61.6.534.

  PMID: 16799133 BACKGROUND

• Choi SJ, Files DC, Zhang T, Wang ZM, Messi ML, Gregory H, Stone J, Lyles MF, Dhar S, Marsh AP, Nicklas BJ, Delbono O. Intramyocellular Lipid and Impaired Myofiber Contraction in Normal Weight and Obese Older Adults. J Gerontol A Biol Sci Med Sci. 2016 Apr;71(4):557-64. doi: 10.1093/gerona/glv169. Epub 2015 Sep 23.

  PMID: 26405061 BACKGROUND

MeSH Terms

Conditions

Obesity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Jae-Youn Lim, Ph.D.

  Seoul National University Bundang Hospital

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, OUTCOMES ASSESSOR
• Purpose: SUPPORTIVE CARE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor, Department of Rehabilitation Medicine

Study Record Dates

• First Submitted: May 10, 2019
• First Posted: May 15, 2019
• Study Start: November 18, 2016
• Primary Completion: February 29, 2020
• Study Completion: February 29, 2020
• Last Updated: March 11, 2020

Record last verified: 2020-03

Data Sharing

• IPD Sharing: Will not share

Locations

KR (1)