Practical Approaches for Interrupting Prolonged Sitting to Improve Postprandial Glucose and Protein Metabolism

NCT03896828 | Completed

• 1 other identifier: SSW
• Study Type: interventional
• Target: 14
• Locations: 1 country
• Sites: 1

Brief Summary

The present study will determine the impact of interrupting prolonged sitting with short, 2-minute walks or body-weight squats on: i) postprandial glycemia and insulinemia, and; ii) postprandial utilization of dietary amino acids. We hypothesize that postprandial glycemia and insulinemia will be similarly improved by interrupting prolonged sitting with short walks or body-weight squats, whereas postprandial utilization of dietary amino acids will only be improved by interrupting prolonged sitting with body-weight squats.

Conditions

Healthy Adults

Outcome Measures

Primary Outcomes (1)

• Postprandial insulin area under the curve (AUC) to breakfast and lunch

  The plasma insulin concentration measured by enzyme-linked immunosorbent assay area under the curve will be measured using the trapezoidal rule.

  3 hours postprandial

Secondary Outcomes (2)

• Postprandial glucose area under the curve (AUC) to breakfast and lunch

  3 hours postprandial

• Skeletal muscle dietary protein incorporation

  7.5 hours postprandial

Study Arms (3)

Sedentary

EXPERIMENTAL

Sedentary (SED): Participants will remain seated in the lab all-day (7.5 hr).

Dietary Supplement: Mixed-macronutrient meal

Walking Breaks

EXPERIMENTAL

Walking breaks (WALK): Participants will perform 2-minute walking breaks at 3.1 mph on a treadmill every 30 minutes (7.5 hr).

Dietary Supplement: Mixed-macronutrient meal

Resistance-exercise breaks

EXPERIMENTAL

Resistance exercise breaks (RE): Participants will perform 15 "squats" (1-minute) every 30 minutes. To reduce the risk of injury, standardize squat-depth, and recruit similar muscle groups as walking, the squats performed will be a "chair-stand with calf-raise" (7.5 hr).

Dietary Supplement: Mixed-macronutrient meal

Interventions

Mixed-macronutrient meal DIETARY_SUPPLEMENT

Participants will be provided a mixed-macronutrient meal (liquid drink) at hours "0" and "3", mimicking the energy and macronutrient composition of breakfast and lunch in Western society (38). 20% of daily energy intake will be provided as "breakfast" and 30% at lunch. Macronutrient composition will reflect a diet providing 55% energy needs from carbohydrate (CHO) and 1.2g/kg/d protein. The remaining energy requirements, as determined by total energy intake, will be met with dietary fat (estimated to be \~0.2 and 0.3g/kg fat for breakfast and lunch, respectively), similar to previous liquid mixed-meal approaches.

Resistance-exercise breaks; Sedentary; Walking Breaks

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Men and women between 18-35 yr
• Recreationally active (≤ 150 minutes of moderate-intensity physical activity per week and no more than 3 days/week of exercise)
• VO2peak considered "good" or below based on ACSM age and sex normative values
• Experiencing regular menstrual periods and not taking hormonal oral contraceptives (females only)

You may not qualify if:

• Inability to perform physical activity as determined by the PAR-Q
• Inability to adhere to protocol guidelines (e.g. alcohol, standardized diet)
• Physical limitations for walking or repeatedly rising from a chair
• Regular tobacco use
• Illicit drug use (e.g. growth hormone, testosterone)
• Hormonal oral contraceptive use (females only)
• Diagnosed medical condition under the care of a physician (e.g. type 2 diabetes)

Sponsors & Collaborators

• University of Toronto: lead

Study Sites (1)

Goldring Center for High Performance Sport

Toronto, Ontario, M5S 2C9, Canada

Location

Study Officials

• Daniel Moore, Ph.D

  University of Toronto

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Model Details: Fourteen men and women will visit the lab on three separate occasions and will remain seated at a desk all-day (Visit 1) or interrupt this sitting every 30-minutes with either 2-minute walking-breaks (Visit 2) or with 15 body-weight squats (repeatedly rising from a chair and onto tip-toes, Visit 3).

Study Record Dates

• First Submitted: March 28, 2019
• First Posted: April 1, 2019
• Study Start: October 19, 2018
• Primary Completion: December 1, 2020
• Study Completion: December 1, 2021
• Last Updated: January 19, 2022

Record last verified: 2022-01

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)