Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization

NCT03747913 | Completed

• 1 other identifier: EISMO
• Study Type: interventional
• Target: 18
• Locations: 1 country
• Sites: 1

Brief Summary

It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated. The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation. The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.

Conditions

Healthy

Outcome Measures

Primary Outcomes (1)

• Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation

  Numbers of hematopoietic, endothelial and mesenchymal stem and progenitor cells and mature endothelial cells in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.

  On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)

Secondary Outcomes (1)

• Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation

  On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)

Study Arms (1)

Antioxidative Supplementation

EXPERIMENTAL

Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation

Dietary Supplement: Antioxidative supplementation

Interventions

Antioxidative supplementation DIETARY_SUPPLEMENT

Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation

Antioxidative Supplementation

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Non-smoking
• Healthy
• Normal Body-Mass-Index (BMI): 18.5-24.9 kg∙m-2
• Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years)
• Willing to adhere to the study rules

You may not qualify if:

• Known or suspected non-compliance, drug or alcohol abuse
• Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant
• Previous enrolment into the current study
• Enrolment of the investigator, his/her family members, employees and other dependent persons
• Regular intake of medication
• Regular intake of antioxidant supplementations

Sponsors & Collaborators

• Swiss Federal Institute of Technology: lead

Study Sites (1)

Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich

Zurich, Canton of Zurich, 8057, Switzerland

Location

Related Publications (1)

• Schmid M, Martins HC, Schratt G, Kropfl JM, Spengler CM. MiRNA126 - RGS16 - CXCL12 Cascade as a Potential Mechanism of Acute Exercise-Induced Precursor Cell Mobilization. Front Physiol. 2021 Dec 9;12:780666. doi: 10.3389/fphys.2021.780666. eCollection 2021.

  PMID: 34955891 DERIVED

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Each participant enrolled in the study will be part of the supplementation and control condition group in a non-randomized order

Study Record Dates

• First Submitted: November 12, 2018
• First Posted: November 20, 2018
• Study Start: May 2, 2019
• Primary Completion: October 21, 2019
• Study Completion: October 31, 2019
• Last Updated: December 16, 2019

Record last verified: 2019-12

Locations

CH (1)