The Effects of Dietary Nitric Oxide and Anthocyanins on Anaerobic Exercise Performance

NCT03631394 | Completed

• 1 other identifier: 5180190
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this graduate student research study is to investigate the effects of nitrate from beetroot powder with additional anthocyanins from tart cherry on exercise performance in healthy Loma Linda University students utilizing the Wingate Anaerobic test on stationary bike.

Conditions

Exercise

Outcome Measures

Primary Outcomes (3)

• Change in blood lactate levels

  Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine the participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at baseline, before and after Wingate Anaerobic test.

  Day 1

• Change in blood lactate levels

  Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at intervention I, before and after Wingate Anaerobic test.

  Day 15

• Change in blood lactate levels

  Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at intervention II, before and after Wingate Anaerobic test.

  Day 29

Secondary Outcomes (1)

• Allantoin blood level

  Baseline, day 15, and day 29

Study Arms (2)

beetroot and anthocyanin

EXPERIMENTAL

A compound pharmacy will formulate capsules with nitrates extracted from beetroot and anthocyanins from tart cherries. A daily dose of the capsules will be taken for 7 days after the washout period. Each dose will comprise 500 mg of nitrates and 450 mg of anthocyanins. In a meta-review by Dominguez and colleagues, 6-8 mmol of nitrates from beetroot was associated with increased exercise performance. Another review by Kelley et al., showed that marathon runners and resistance trainers ingesting between 450-480 mg of anthocyanins had reduced muscle soreness and decreased oxidative stress. Subject will consume each supplement orally with only water 2 hours pre-prandial.

Other: beetroot and anthocyanin

beetroot and placebo

PLACEBO COMPARATOR

The same compound pharmacy will formulate capsules with nitrate extracted from beetroot and a placebo element made of starch. This product will taste the same as the nitrate and anthocyanin supplementation. A daily dose of the capsules will be taken for 7 days after the washout period. Each supplementation will have 500 mg of nitrate and 450 mg of placebo. Subjects will consume each supplement orally with only water 2 hours pre-prandial.

Other: beetroot and placebo

Interventions

beetroot and anthocyanin OTHER

Beetroot and anthocyanin will be taken for 7 days during the study two hours prior to eating.

beetroot and anthocyanin

beetroot and placebo OTHER

Beetroot and placebo will be taken for 7 days during the study two hours prior to eating.

beetroot and placebo

Eligibility Criteria

• Age: 21 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy Loma Linda University students aged between 21 and 35 years old
• Willing to abstain from caffeine, mouthwash, chewing gums, and high antioxidant foods during the week of supplementation.

You may not qualify if:

• Diabetic
• having salivary gland disorder, cardiovascular disease or orthopedic injuries
• pregnant or breastfeeding
• taking NSAIDS
• have an allergy to anthocyanins and/or beetroot
• women who are on their menses

Sponsors & Collaborators

• Loma Linda University: lead

Study Sites (1)

Loma Linda Universtiy

Loma Linda, California, 92350, United States

Location

MeSH Terms

Conditions

Motor Activity

Interventions

Anthocyanins

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Flavonoids; Chromones; Benzopyrans; Pyrans; Heterocyclic Compounds, 1-Ring; Heterocyclic Compounds; Heterocyclic Compounds, 2-Ring; Heterocyclic Compounds, Fused-Ring; Glycosides; Carbohydrates; Pigments, Biological; Biological Factors

Study Officials

• Edward Bitok, DrPH

  Loma Linda University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: June 19, 2018
• First Posted: August 15, 2018
• Study Start: September 1, 2018
• Primary Completion: April 1, 2019
• Study Completion: April 1, 2019
• Last Updated: May 15, 2019

Record last verified: 2019-05

Locations

US (1)