The Impact of Acute (2 h) and Short-term (4 Days) Nitrate Supplementation in the Form of Red Beet Root Juice on Intense Intermittent Exercise Performance.

NCT03616093 | Completed

• 1 other identifier: PEP-1718
• Study Type: interventional
• Target: 16
• Locations: 1 country
• Sites: 1

Brief Summary

Both acute ingestion (1-3 h) and chronic supplementation (3-30 days) with dietary nitrate has been shown to increase nitric oxide activity and, in some studies, to improve exercise economy, exercise tolerance, and endurance exercise performance. In addition, more recent evidence suggests that dietary nitrate has the potential to enhance team-sport-specific high-intensity intermittent exercise performance. Indeed, chronic dietary nitrate supplementation (2-6 days) has been reported to increase the distance covered before exhaustion during the Yo-Yo intermittent recovery level 1 test, a well-established and ecologically valid test widely used to mimic the high-intensity running bouts of soccer match-play. However, while these findings suggest that nitrate may be an effective ergogenic aid for team-sport players when consumed daily, it is currently unclear if the improvement in team-sport specific intermittent exercise performance can be achieved following a single bolus of dietary nitrate. Given the increasing interest and use of dietary nitrate as an ergogenic aid by team-sport players, establishing the shortest period of supplementation required to elicit an ergogenic effect is important in order to guide athletes on optimal supplementation strategies. Given that a single bolus of dietary nitrate has previously been shown to improve exercise tolerance and endurance exercise performance, and an improved Yo-Yo IR1 performance has been observed after chronic nitrate supplementation, the investigator hypothesizes that acute (2 h) and short-term (4 days) nitrate supplementation will significantly elevate nitric oxide activity (measured as an increase in plasma nitrite concentration and skeletal muscle nitrate concentration) and improve performance during the Yo-Yo IR1 test (measured as an increased in distance covered (m) during the test at the point of volitional exhaustion) to a similar extent, when compared to a placebo.

Conditions

Exercise Performance

Outcome Measures

Primary Outcomes (2)

• Distance covered during high-intensity intermittent exercise performance

  4 days

• Distance covered during high-intensity intermittent exercise performance

  2 hours

Secondary Outcomes (2)

• Plasma nitrate concentrations

  4 days

• Nitrite concentrations

  2 hours

Study Arms (2)

Test Beet shot

EXPERIMENTAL

Active supplement containing 6.2 mmol nitrate

Other: Test Beet Shot

Placebo beverage

PLACEBO COMPARATOR

Placebo supplement containing negligible nitrate

Other: Placebo Beverage

Interventions

Test Beet Shot OTHER

Active supplement containing 6.2 mmol nitrate

Test Beet shot

Placebo Beverage OTHER

Placebo supplement containing negligible nitrate

Placebo beverage

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male, physically active, intermittent team sport players, in self-reported good general health as assessed by the standard procedures described below and specifically meeting the following: blood pressure (BP), systolic 100-135 mmHg and diastolic 60-90 mmHg, and; body mass index (BMI) 16-29.9.
• years of age
• Participants must participate in an intermittent exercise based sport (e.g. soccer, rugby, hockey), at a competitive level (i.e. competes in officiated matches/games).
• Understanding of the procedures to be undertaken as part of the study
• Willingness to participate in exercise testing and follow supplementation guidelines and other instructions provided by the experimenter
• Informed, voluntary, written consent to participate in the study

You may not qualify if:

• Known pulmonary, cardiovascular or metabolic disease
• Food allergies including phenylketonurea (PKU)
• Current regular use of any dietary supplement (excluding macronutrient based supplements) or previous use of any dietary supplement in the past 6 months that is known to have a lasting effect.
• Blood donation within 3 months prior to the start of the study
• Substance abuse within 2 years of the start of the study
• Smoking
• Any prescription and non-prescription medication which may impact saliva production (antidepressants, diuretics, analgesics, antihistamines, anti-hyper/hypo-tensives, anti-anxiety, appetite suppressants).
• Current or previous use of phosphodiesterase 5 (PDE5) inhibitors.
• Participation in another clinical trial within past 4 weeks and/or participation in a PepsiCo trial within the last 6 months.
• Chronic use (6 months) of any antibacterial mouthwash products.

Sponsors & Collaborators

• PepsiCo Global R&D: lead

Study Sites (1)

University of Exeter, Sport and Health Sciences department,

Exeter, United Kingdom

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: INDUSTRY
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: June 6, 2018
• First Posted: August 6, 2018
• Study Start: September 30, 2017
• Primary Completion: May 1, 2018
• Study Completion: May 31, 2018
• Last Updated: August 6, 2018

Record last verified: 2018-08

Locations

GB (1)