TNF-α/HIF-1α/VEGF Pathway in Periodontal Diseases

NCT03558906 | Completed

• 1 other identifier: bafacan357
• Study Type: observational
• Target: 87
• Locations: 0 countries
• Sites: N/A

Brief Summary

This study aimed to investigate gingival crevicular fluid (GCF) and salivary hypoxia inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-α) levels in different periodontal diseases. A total of 87 individuals, 20 patients with generalized aggressive periodontitis, 20 with chronic periodontitis, 26 with gingivitis and 21 periodontally healthy individuals were included. Whole-mouth and site-specific clinical periodontal parameters including probing depth, clinical attachment level, bleeding on probing, gingival index and plaque index were recorded. GCF and salivary HIF-1α, VEGF and TNF-α levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was performed by using non-parametric tests.

Conditions

Periodontal Diseases

Keywords

Periodontal disease; Hypoxia; Cytokines; Gingival crevicular fluid; Saliva

Outcome Measures

Primary Outcomes (1)

• Gingival crevicular fluid HIF-1α levels

  pg

  A day after clinical periodontal examination

Secondary Outcomes (3)

• Salivary HIF-1α levels

  A day after clinical periodontal examination

• Gingival crevicular fluid VEGF levels

  A day after clinical periodontal examination

• Salivary VEGF levels

  A day after clinical periodontal examination

Study Arms (4)

Aggressive Periodontitis

These individuals had minimum PD ≥6 mm and CAL ≥5 mm on eight or more teeth; at least three of these were other than central incisors or first molars. Radiographic alveolar bone loss was ≥30% of root length affecting at least three permanent teeth other than first molars and incisors. The severe destruction pattern was not commensurate with amount of plaque accumulation.

Chronic periodontitis

These individuals had at least four non-adjacent teeth with sites with PD ≥6 mm and CAL ≥5 mm. They had also ≥50% alveolar bone loss in at least two quadrants that was commensurate with amount of plaque accumulation. BOP was \>50% in the whole mouth.

Gingivitis

Gingivitis patients exhibited no sites with CAL \>2 mm and no detectable alveolar bone loss in the radiography. BOP was \>50% in the whole mouth.

Healthy

Periodontally healthy volunteers exhibited no sites with PD \>3 mm and CAL \>2 mm as well as no radiographic evidence of alveolar bone loss. BOP was \<15% in the whole mouth.

Eligibility Criteria

• Age: 27 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

All consecutive individuals were recruited from the Department of Periodontology, School of Dentistry, Adnan Menderes University, Aydın.

You may qualify if:

• Systemically healthy individuals
• Non-smoker individuals

You may not qualify if:

• Current pregnancy or lactation
• Nonsurgical/surgical periodontal treatment received for the past year
• Use of anti-inflammatory and antibiotic drugs within the past 6 months.

Sponsors & Collaborators

• Aydin Adnan Menderes University: lead
• Celal Bayar University: collaborator
• Ege University: collaborator

Related Publications (1)

• Afacan B, Ozturk VO, Pasali C, Bozkurt E, Kose T, Emingil G. Gingival crevicular fluid and salivary HIF-1alpha, VEGF, and TNF-alpha levels in periodontal health and disease. J Periodontol. 2019 Jul;90(7):788-797. doi: 10.1002/JPER.18-0412. Epub 2018 Dec 11.

  PMID: 30536725 DERIVED

Biospecimen

Retention: SAMPLES WITHOUT DNA

Gingival crevicular fluid, saliva

MeSH Terms

Conditions

Periodontal Diseases; Hypoxia

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases; Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: May 23, 2018
• First Posted: June 15, 2018
• Study Start: March 1, 2016
• Primary Completion: March 2, 2016
• Study Completion: December 26, 2016
• Last Updated: June 15, 2018

Record last verified: 2018-06