Analysis of Gingival Crevicular Fluid

NCT05325905 | Completed

• 1 other identifier: 2014/01
• Study Type: observational
• Target: 81
• Locations: 0 countries
• Sites: N/A

Brief Summary

The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.

Conditions

Periodontal Diseases

Keywords

pathogenesis; Gingival crevicular fluid; cytokine

Outcome Measures

Primary Outcomes (1)

• Interleukin-36γ (IL-36γ) levels in GCF

  GCF samples were obtained from one interproximal site in each quadrant

  Baseline

Secondary Outcomes (6)

• Probing depth (PD)

  Baseline

• Clinical attachment level (CAL)

  Baseline

• Bleeding on Probing (BoP)

  Baseline

• IL-10 and IL-1β levels in GCF

  Baseline

• Plaque Index (PI)

  Baseline

Study Arms (3)

Healthy

bleeding on probing (BoP) ≤ 10% of the sites, probing depth (PD) ≤ 3 mm and no indication of clinical attachment level (CAL) or radiographic bone loss

Diagnostic Test: Gingival crevicular fluid sampling

Gingivitis

gingival inflammation (BoP ≥ 10% of the sites, red-blue discoloration of the gingiva and edema) PD ≤ 4 mm and no clinical attachment or radiographic bone loss

Diagnostic Test: Gingival crevicular fluid sampling

Periodontitis

PD ≤ 5 mm, radiographic bone loss, and 3-4 mm CAL in at least 30% of the sites

Diagnostic Test: Gingival crevicular fluid sampling

Interventions

Gingival crevicular fluid sampling DIAGNOSTIC_TEST

GCF sampling and clinical periodontal measurements were performed

Gingivitis; Healthy; Periodontitis

Eligibility Criteria

• Age: 20 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients were selected from among admitting to Dentistry Faculty of Selcuk University.

You may qualify if:

• Systemically healthy
• non-smokers,
• presence of at least 20 teeth in the mouth

You may not qualify if:

• receiving periodontal therapy in the last 6 months;
• receiving antibiotics or anti-inflammatory drugs in the last 6 months;
• currently pregnant or breastfeeding
• using a removable partial denture and removable or fixed orthodontic appliances.

Sponsors & Collaborators

• Bezmialem Vakif University: lead
• Selcuk University: collaborator

Biospecimen

Retention: SAMPLES WITHOUT DNA

Gingival crevicular fluid

MeSH Terms

Conditions

Periodontal Diseases

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases

Study Officials

• Mehtikar Gürsel

  Bezmialem University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Prof.Dr

Study Record Dates

• First Submitted: April 6, 2022
• First Posted: April 13, 2022
• Study Start: October 1, 2014
• Primary Completion: April 1, 2016
• Study Completion: January 1, 2017
• Last Updated: April 13, 2022

Record last verified: 2022-04

Data Sharing

• IPD Sharing: Will not share