NCT03338972

Brief Summary

This phase I trial studies the side effects and best dose of BCMA CAR-T cells in treating patients with BCMA positive multiple myeloma that has come back or does not respond to treatment. T cells are a type of white blood cell and a major component of the immune system. T-cells that have been genetically modified in the laboratory express BCMA and may kill cancer cells with the protein BCMA on their surface. Giving chemotherapy before BCMA CAR-T cells may reduce the amount of disease and to cause a low lymphocyte (white blood cell) count in the blood, which may help the infused BCMA CAR-T cells survive and expand.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
28

participants targeted

Target at P25-P50 for phase_1

Timeline
Completed

Started Nov 2017

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

November 7, 2017

Completed
2 days until next milestone

First Posted

Study publicly available on registry

November 9, 2017

Completed
20 days until next milestone

Study Start

First participant enrolled

November 29, 2017

Completed
3.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 3, 2021

Completed
11 months until next milestone

Study Completion

Last participant's last visit for all outcomes

March 22, 2022

Completed
5 months until next milestone

Results Posted

Study results publicly available

August 5, 2022

Completed
Last Updated

September 8, 2023

Status Verified

August 1, 2023

Enrollment Period

3.4 years

First QC Date

November 7, 2017

Results QC Date

April 26, 2022

Last Update Submit

August 15, 2023

Conditions

Outcome Measures

Primary Outcomes (2)

  • Dose-limiting Toxicities (DLT) Rate

    Observed DLT rates will be summarized based on the DLT-Evaluable analysis set. Outcome will be reported as count of participants in each arm who experienced a DLT. No patients received more than one CAR T cell infusion, so DLT assessment period was only 28 days after first and only CAR T infusion for all patients.

    Up to 28 days after CAR T cell infusion

  • Count of Patients That Experienced Adverse Events

    Toxicity graded using National Cancer Institute (NCI) Common Terminology Criteria for Adverse Events (CTCAE) version 4.03 No patients received more than one CAR T cell infusion, so AE assessment period was only 28 days after first and only CAR T infusion for all patients.

    Up to 28 days after CAR T-cell infusion

Secondary Outcomes (5)

  • Duration of Persistence of Adoptively Transferred BCMA CAR-T Cells

    Assessed from Baseline up to a maximum of 537 days

  • Number of Participants With Detectable BCMA CART Cell Migration to Primary Disease Site (Bone Marrow) at Day 28

    Baseline up to Day 28

  • Objective Response Rate (ORR)

    Baseline up to 3 months after CART infusion

  • Progression-free Survival (PFS)

    Assessed up to 1 year after CART infusion

  • Overall Survival (OS)

    Assessed up to 1 year after CART infusion

Study Arms (4)

Treatment (chemotherapy, BCMA CAR-T cells) at dose level 1

EXPERIMENTAL

Patients undergo leukapheresis. Patients then receive cyclophosphamide and fludarabine on days -4 to -2. Beginning to 36-96 days after chemotherapy, patients receive BCMA-specific CAR-expressing T lymphocytes IV over 20-30 minutes on day 0. Patients may receive a second dose of BCMA-specific CAR-expressing T lymphocytes IV with or without additional cytoreductive chemotherapy at the discretion of the principal investigator or their designee (sub-investigator). Arm 1 contains patients treated as dose level 1 (50 x 10\^6 EGFRt cells)

Biological: Autologous Anti-BCMA-CAR-expressing CD4+/CD8+ T-lymphocytes FCARH143Drug: CyclophosphamideDrug: FludarabineProcedure: Leukapheresis

Treatment (chemotherapy, BCMA CAR-T cells) at dose level 2

EXPERIMENTAL

Patients undergo leukapheresis. Patients then receive cyclophosphamide and fludarabine on days -4 to -2. Beginning to 36-96 days after chemotherapy, patients receive BCMA-specific CAR-expressing T lymphocytes IV over 20-30 minutes on day 0. Patients may receive a second dose of BCMA-specific CAR-expressing T lymphocytes IV with or without additional cytoreductive chemotherapy at the discretion of the principal investigator or their designee (sub-investigator). Arm 2 contains patients treated as dose level 2 (150 x 10\^6 EGFRt cells)

Biological: Autologous Anti-BCMA-CAR-expressing CD4+/CD8+ T-lymphocytes FCARH143Drug: CyclophosphamideDrug: FludarabineProcedure: Leukapheresis

Treatment (chemotherapy, BCMA CAR-T cells) at dose level 3

EXPERIMENTAL

Patients undergo leukapheresis. Patients then receive cyclophosphamide and fludarabine on days -4 to -2. Beginning to 36-96 days after chemotherapy, patients receive BCMA-specific CAR-expressing T lymphocytes IV over 20-30 minutes on day 0. Patients may receive a second dose of BCMA-specific CAR-expressing T lymphocytes IV with or without additional cytoreductive chemotherapy at the discretion of the principal investigator or their designee (sub-investigator). Arm 3 contains patients treated as dose level 3 (300 x 10\^6 EGFRt cells)

Biological: Autologous Anti-BCMA-CAR-expressing CD4+/CD8+ T-lymphocytes FCARH143Drug: CyclophosphamideDrug: FludarabineProcedure: Leukapheresis

Treatment (chemotherapy, BCMA CAR-T cells) at dose level 4

EXPERIMENTAL

Patients undergo leukapheresis. Patients then receive cyclophosphamide and fludarabine on days -4 to -2. Beginning to 36-96 days after chemotherapy, patients receive BCMA-specific CAR-expressing T lymphocytes IV over 20-30 minutes on day 0. Patients may receive a second dose of BCMA-specific CAR-expressing T lymphocytes IV with or without additional cytoreductive chemotherapy at the discretion of the principal investigator or their designee (sub-investigator). Arm 4 contains patients treated as dose level 4 (450 x 10\^6 EGFRt cells)

Biological: Autologous Anti-BCMA-CAR-expressing CD4+/CD8+ T-lymphocytes FCARH143Drug: CyclophosphamideDrug: FludarabineProcedure: Leukapheresis

Interventions

Given IV

Also known as: Autologous Anti-BCMA-CAR CD4+/CD8+ Cells, Autologous Anti-BCMA-CAR-expressing CD8+ and CD4+ T-lymphocytes, BCMA CAR-CD4+/CD8+ T-cells, BCMA-specific CAR-expressing CD4+/CD8+ T-lymphocytes, FCARH143
Treatment (chemotherapy, BCMA CAR-T cells) at dose level 1Treatment (chemotherapy, BCMA CAR-T cells) at dose level 2Treatment (chemotherapy, BCMA CAR-T cells) at dose level 3Treatment (chemotherapy, BCMA CAR-T cells) at dose level 4

Given IV

Also known as: (-)-Cyclophosphamide, 2H-1,3,2-Oxazaphosphorine, 2-[bis(2-chloroethyl)amino]tetrahydro-, 2-oxide, monohydrate, Carloxan, Ciclofosfamida, Ciclofosfamide, Cicloxal, Clafen, Claphene, CP monohydrate, CTX, CYCLO-cell, Cycloblastin, Cycloblastine, Cyclophospham, Cyclophosphamid monohydrate, Cyclophosphamidum, Cyclophosphan, Cyclophosphane, Cyclophosphanum, Cyclostin, Cyclostine, Cytophosphan, Cytophosphane, Cytoxan, Fosfaseron, Genoxal, Genuxal, Ledoxina, Mitoxan, Neosar, Revimmune, Syklofosfamid, WR- 138719
Treatment (chemotherapy, BCMA CAR-T cells) at dose level 1Treatment (chemotherapy, BCMA CAR-T cells) at dose level 2Treatment (chemotherapy, BCMA CAR-T cells) at dose level 3Treatment (chemotherapy, BCMA CAR-T cells) at dose level 4

Given IV

Also known as: Fluradosa
Treatment (chemotherapy, BCMA CAR-T cells) at dose level 1Treatment (chemotherapy, BCMA CAR-T cells) at dose level 2Treatment (chemotherapy, BCMA CAR-T cells) at dose level 3Treatment (chemotherapy, BCMA CAR-T cells) at dose level 4
LeukapheresisPROCEDURE

Undergo leukapheresis

Also known as: Leukocytopheresis, Therapeutic Leukopheresis
Treatment (chemotherapy, BCMA CAR-T cells) at dose level 1Treatment (chemotherapy, BCMA CAR-T cells) at dose level 2Treatment (chemotherapy, BCMA CAR-T cells) at dose level 3Treatment (chemotherapy, BCMA CAR-T cells) at dose level 4

Eligibility Criteria

Age21 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Have the capacity to give informed consent
  • Eastern Cooperative Oncology Group (ECOG) performance status score =\< 2.
  • Have measurable disease by International Myeloma Working Group (IMWG) criteria based on one or more of the following findings:
  • Serum M-protein \>= 1 g/dL
  • Urine M-protein \>= 200 mg/24 hour
  • Involved serum free light chain (sFLC) level \>= 10 mg/dL with abnormal kappa/lambda ratio
  • Measurable biopsy-proven plasmacytomas (\>= 1 lesion that has a single diameter \>= 2 cm)
  • Bone marrow plasma cells \>= 30%
  • Have a diagnosis of BCMA+ MM (\>= 5% BCMA+ by flow cytometry on CD138 co-expressing plasma cells obtained within 45 days of study enrollment); the MM diagnosis must be confirmed by internal pathology review of a fresh biopsy specimen at the Fred Hutchinson Cancer Research Center (FHCRC)/Seattle Cancer Care Alliance (SCCA)
  • Have relapsed or treatment refractory disease with \>= 10% CD138+ malignant plasma cells (IHC) on BM core biopsy, either:
  • Following autologous stem cell transplant (ASCT)
  • Or, if a patient has not yet undergone ASCT, the individual must:
  • Be transplant ineligible, due to age, comorbidity, patient choice, insurance reasons, concerns of rapidly progressive disease, and/or discretion of attending physician and principal investigator and,
  • Demonstrate disease that persists after \> 4 cycles of induction therapy and that is double refractory (persistence/progression) after therapy with both a proteasome inhibitor and immunomodulatory drug (IMiD) administered either in tandem, or in sequence; \> 4 cycles of therapy are not required for patients with a diagnosis of plasma cell leukemia
  • Patients receiving retreatment do not need to meet the \> 10% CD138+ malignant plasma cells (immunohistochemistry staining method \[IHC\]) on BM core biopsy
  • +1 more criteria

You may not qualify if:

  • History of another primary malignancy that requires intervention beyond surveillance or that has not been in remission for at least 1 year (the following are exempt from the 1 year limit: non-melanoma skin cancer, curatively treated localized prostate cancer, and cervical carcinoma in situ on biopsy or a squamous intraepithelial lesion on papanicolaou \[PAP\] smear)
  • Active hepatitis B, hepatitis C at the time of screening
  • Patients who are (human immunodeficiency virus \[HIV\]) seropositive
  • Subjects with uncontrolled active infection
  • \> 1 hospital admission (lasting 5 days or more) for documented infection in prior 6 months
  • Presence of acute or chronic graft-versus-host disease (GVHD) requiring active treatment unless limited to skin involvement and managed with topical steroid therapy alone
  • History of any one of the following cardiovascular conditions within the past 6 months: Class III or IV heart failure as defined by the New York Heart Association (NYHA), cardiac angioplasty or stenting, myocardial infarction, unstable angina, or other clinically significant cardiac disease as determined by the principal investigator (PI) or designee
  • History of clinically relevant or active central nervous system (CNS) pathology such as epilepsy, seizure, paresis, aphasia, stroke, severe brain injuries, dementia, Parkinson's disease, cerebellar disease, organic brain syndrome, or psychosis, active central nervous system MM involvement and/or carcinomatous meningitis; subjects with previously treated central nervous systems involvement may participate, provided they are free of disease in the CNS (documented by flow cytometry performed on the cerebrospinal fluid \[CSF\] within 14 days of enrollment) and have no evidence of new sites of CNS activity
  • Pregnant or breastfeeding females
  • Allogeneic HSCT or donor lymphocyte infusion within 90 days of leukapheresis.
  • Use of any of the following:
  • Therapeutic doses of corticosteroids (defined as \> 20 mg/day prednisone or equivalent) within 7 days prior to leukapheresis; physiologic replacement, topical, and inhaled steroids are permitted
  • Cytotoxic chemotherapeutic agents within 1 week of leukapheresis; oral chemotherapeutic agents are allowed if at least 3 half-lives have elapsed prior to leukapheresis
  • Lymphotoxic chemotherapeutic agents within 2 weeks of leukapheresis
  • Daratumumab (or other anti-CD38 therapy) within 30 days of leukapheresis
  • +13 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Fred Hutch/University of Washington Cancer Consortium

Seattle, Washington, 98109, United States

Location

Related Publications (1)

  • Tuazon SA, Portuguese AJ, Pont MJ, Cowan AJ, Cole GO, Sather BD, Song X, Thomas S, Wood BL, Blake M, Works MG, Shadman M, Liang EC, Wu QV, Voutsinas JM, Gooley TA, Turtle CJ, Till BG, Coffey DG, Maloney DG, Riddell SR, Green DJ. A phase 1 trial of fully human BCMA CAR-T therapy for relapsed/refractory multiple myeloma with 5-year follow-up. Blood. 2025 Jul 31;146(5):535-545. doi: 10.1182/blood.2024027681.

MeSH Terms

Conditions

Multiple Myeloma

Interventions

CyclophosphamidefludarabineLeukapheresis

Condition Hierarchy (Ancestors)

Neoplasms, Plasma CellNeoplasms by Histologic TypeNeoplasmsHemostatic DisordersVascular DiseasesCardiovascular DiseasesParaproteinemiasBlood Protein DisordersHematologic DiseasesHemic and Lymphatic DiseasesHemorrhagic DisordersLymphoproliferative DisordersImmunoproliferative DisordersImmune System Diseases

Intervention Hierarchy (Ancestors)

Phosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus CompoundsCytapheresisBiological TherapyTherapeuticsBlood Component RemovalLeukocyte Reduction ProceduresCell SeparationCytological TechniquesClinical Laboratory TechniquesInvestigative Techniques

Results Point of Contact

Title
Dr. Damian Green
Organization
Fred Hutchinson Cancer Center

Study Officials

  • Damian J. Green

    Fred Hutch/University of Washington Cancer Consortium

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
Yes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

November 7, 2017

First Posted

November 9, 2017

Study Start

November 29, 2017

Primary Completion

May 3, 2021

Study Completion

March 22, 2022

Last Updated

September 8, 2023

Results First Posted

August 5, 2022

Record last verified: 2023-08

Locations