Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein (ICADP)

NCT03303729 | Completed DMC

• 1 other identifier: H-17017363
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin. The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin. Hypotheses: I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period. II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.

Conditions

Muscle Protein Synthesis; Amino Acid

Keywords

Carbohydrate; Cluster Dextrin; Exercise training; Protein supplement

Outcome Measures

Primary Outcomes (1)

• Protein hydrolysate amino acid label time to peak appearance in plasma

  Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma

  From hour 0 to hour 3

Secondary Outcomes (3)

• Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise

  from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3.

• Protein hydrolysate amino acid label area under the concentration curve in plasma

  From hour 0 to hour 3

• Insulin concentration

  From hour 0 to hour 3

Study Arms (2)

Meat hydrolysate & Cluster Dextrin

EXPERIMENTAL

Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin.

Dietary Supplement: Meat Hydrolysate; Dietary Supplement: Cluster Dextrin

Meat hydrolysate & placebo

ACTIVE COMPARATOR

Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose.

Dietary Supplement: Meat Hydrolysate; Dietary Supplement: Placebo

Interventions

Meat Hydrolysate DIETARY_SUPPLEMENT

25 grams of meat hydrolysate to maximally stimulate protein synthesis.

Meat hydrolysate & Cluster Dextrin; Meat hydrolysate & placebo

Cluster Dextrin DIETARY_SUPPLEMENT

75 grams of Cluster Dextrin

Also known as: CAS no.: 9050-36-6, Glico Nutrition Co., Ltd.

Meat hydrolysate & Cluster Dextrin

Placebo DIETARY_SUPPLEMENT

75 grams of Glucose

Also known as: Glucose

Meat hydrolysate & placebo

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• young healthy adults,
• organised sport/training 1-3 times per week

You may not qualify if:

• Subject younger than 18 years or above 30 years
• BMI \> 30
• Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus.
• Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week.
• Participating in systematized exercise more than 3 times per week.
• Vegetarian.
• Participating in a special diet which deviates from the standard of young adults.

Sponsors & Collaborators

• Bispebjerg Hospital: lead
• University of Birmingham: collaborator

Study Sites (1)

Bispebjerg Hospital

Copenhagen, Copenhagen NV, 2400, Denmark

Location

Related Publications (1)

• Nishimura Y, Jensen M, Bulow J, Thomsen TT, Arimitsu T, van Hall G, Fujita S, Holm L. Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. Eur J Nutr. 2022 Aug;61(5):2475-2491. doi: 10.1007/s00394-021-02782-y. Epub 2022 Feb 19.

  PMID: 35182194 DERIVED

MeSH Terms

Interventions

Long-Term Synaptic Depression; Glucose

Intervention Hierarchy (Ancestors)

Neuronal Plasticity; Nervous System Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Hexoses; Monosaccharides; Sugars; Carbohydrates

Study Officials

• Jacob L. Bülow, MD

  Bispebjerg Hospital

  PRINCIPAL INVESTIGATOR

• Lars Holm, Prof.

  University of Birmingham

  PRINCIPAL INVESTIGATOR

• Michael Kjaer, MD, prof.

  Bispebjerg Hospital

  PRINCIPAL INVESTIGATOR

• Sofus Sølyst, stud.med.

  University of Copenhagen

  PRINCIPAL INVESTIGATOR

• Mikkel Jensen, Stud.scient.

  University of Copenhagen

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD, MSc

Study Record Dates

• First Submitted: October 3, 2017
• First Posted: October 6, 2017
• Study Start: November 24, 2017
• Primary Completion: February 23, 2018
• Study Completion: February 23, 2018
• Last Updated: February 27, 2018

Record last verified: 2018-02

Locations

DK (1)