NCT03302403

Brief Summary

A single arm, open-label pilot study is designed to determine the safety, efficacy and cytokinetics of CAR T cells in patients with malignant tumors with positive antigen targets. CAR T cells are genetically engineered to express single-chain variable fragment (scFv) targeting indication-specific antigens. The investigational CAR T cells and proposed indications are as follows: CAR-CD19 T cells for B cell leukaemia/lymphoma; CAR-BCMA T cells for myeloma; CAR-GPC3 T cell for hepatocellular carcinoma; CAR-CLD18 T cells for pancreatic carcinoma and adenocarcinoma of esophagogastric junction.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
18

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Dec 2017

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

September 26, 2017

Completed
9 days until next milestone

First Posted

Study publicly available on registry

October 5, 2017

Completed
3 months until next milestone

Study Start

First participant enrolled

December 29, 2017

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 12, 2020

Completed
3 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 12, 2023

Completed
Last Updated

October 8, 2020

Status Verified

October 1, 2020

Enrollment Period

3 years

First QC Date

September 26, 2017

Last Update Submit

October 5, 2020

Conditions

Outcome Measures

Primary Outcomes (1)

  • Number of participants with CRA T-related adverse events as assessed by CTCAE v4.03

    Number of participants with study related adverse events which are defined as laboratory toxicities and clinical events that are possible, likely or definitely related to study treatment at any time from the infusion until week 24, including infusion related toxicity and any toxicity possibly related to CAR T cells.

    24 weeks

Secondary Outcomes (1)

  • Engraftment

    2 years

Other Outcomes (8)

  • Progression-free Survival (PFS)

    5 years for BCMA, 2 years for GPC3, CD19 and Claudin 18.2

  • Time to Tumor Progression (TTP)

    5 years for BCMA, 2 years for GPC3, CD19 and Claudin 18.2

  • Disease Control Rate (DCR)

    2 years

  • +5 more other outcomes

Study Arms (1)

CAR T cell

EXPERIMENTAL

In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors: CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction. Route of administration: Intravenous injection. Lymphodepletion conditioning: Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells. A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.

Genetic: CAR-CD19 T cellGenetic: CAR-BCMA T cellGenetic: CAR-GPC3 T cellGenetic: CAR-CLD18 T cellDrug: FludarabineDrug: Cyclophosphamide

Interventions

Self-controlled dose escalation and classic "3+3" dose escalation will be applied.

Also known as: CD19-redirected Autologous Cell
CAR T cell

Self-controlled dose escalation and classic "3+3" dose escalation will be applied.

Also known as: BCMA-redirected Autologous Cell
CAR T cell

Self-controlled dose escalation and classic "3+3" dose escalation will be applied.

Also known as: GPC3-redirected Autologous Cell
CAR T cell

Self-controlled dose escalation and classic "3+3" dose escalation will be applied.

Also known as: Claudin18.2-redirected Autologous Cell
CAR T cell

Fludarabine is used for lymphodepletion.

CAR T cell

Cyclophosphamide is used for lymphodepletion.

CAR T cell

Eligibility Criteria

Age18 Years - 75 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • I. B-Cell Lymphoblastic Leukaemia/Lymphoma
  • Patients aged between 18 \~ 65 with B-cell lymphoblastic leukaemia/lymphoma.
  • CD19-positive B-cell lymphoblastic leukaemia/lymphoma.
  • Patients with unmet medical needs for which there are no effective therapies known at this time:
  • A. Relapsed or Refractory (r/r) Acute Lymphoblastic Leukemia (ALL)
  • Patients with r/r ALL for whom hematopoietic stem cell transplantation (HSCT) is not suitable due to following reasons:
  • Age;
  • Excessive tumor burden or concomitant disease;
  • No donor available.
  • B. CD19-positive Follicular Lymphoma:
  • At least 2 prior chemotherapy regimens (not including single agent monoclonal antibody (Rituxan) therapy;
  • Less than 6 months between last chemotherapy and disease progression (most recent progression free interval \< 6 months);
  • Disease progression after most recent systemic therapy (chemotherapy, MoAb, etc.).
  • C. Chronic Lymphocytic Leukemia (CLL)
  • At least 2 prior chemotherapy regimens (not including single agent monoclonal antibody (Rituxan) therapy);
  • +76 more criteria

You may not qualify if:

  • Patients with any of the following conditions are not eligible for this study.
  • Transduction of target lymphocytes \< 10%, expansion in response to αCD3/CD28 costimulation \< 5-fold.
  • Pregnant or lactating women.
  • HIV positive, or HCV positive
  • Uncontrolled active infection, including active tuberculosis and HBV DNA copies ≥ 1×10\^3 copies/mL.
  • Allergic to immunotherapies and related drugs.
  • Patients with heart disease for which treatment is needed or with poorly controlled hypertension.
  • Hyponatremia: serum sodium level \< 125 mmol/L.
  • Baseline serum potassium \< 3.5 mmol/L (taking potassium supplements before participating in the study to raise potassium level is acceptable).
  • Previous treatment with chemoradiotherapy, immunotherapy and tumor-targeting drug conducted 2 weeks prior to participation in this study or blood collection.
  • Patients have undertaken immunosuppressor for graft-versus-host disease (GVHD) within 4 weeks before participation in this study or blood collection, or the patient is diagnosed with acute or chronic GVHD.
  • Other severe disease that may restrain patients from participating in this study (e.g. diabetes, severe cardiac dysfunction, myocardial infarction or unstable arrhythmias or unstable angina in recent 6 months, gastric ulcer, active autoimmune disease, etc.).

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

First Affiliated Hospital of Wenzhou Medical University

Wenzhou, Zhejiang, 325000, China

Location

Related Publications (1)

  • Dong R, Jiang S, Chen Y, Ma Y, Sun L, Xing C, Zhang S, Yu K. Prognostic Significance of Cytokine Release Syndrome in B Cell Hematological Malignancies Patients After Chimeric Antigen Receptor T Cell Therapy. J Interferon Cytokine Res. 2021 Dec;41(12):469-476. doi: 10.1089/jir.2021.0057.

MeSH Terms

Conditions

Lymphoma, B-CellLeukemia, B-CellNeoplasms, Plasma CellCarcinoma, HepatocellularPancreatic Neoplasms

Interventions

fludarabineCyclophosphamide

Condition Hierarchy (Ancestors)

Lymphoma, Non-HodgkinLymphomaNeoplasms by Histologic TypeNeoplasmsLymphoproliferative DisordersLymphatic DiseasesHemic and Lymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesLeukemia, LymphoidLeukemiaHematologic DiseasesAdenocarcinomaCarcinomaNeoplasms, Glandular and EpithelialLiver NeoplasmsDigestive System NeoplasmsNeoplasms by SiteDigestive System DiseasesLiver DiseasesEndocrine Gland NeoplasmsPancreatic DiseasesEndocrine System Diseases

Intervention Hierarchy (Ancestors)

Phosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus Compounds

Study Officials

  • Mengtao Zhou, MD

    First Affiliated Hospital of Wenzhou Medical University

    PRINCIPAL INVESTIGATOR
  • Kang Yu, MD

    First Affiliated Hospital of Wenzhou Medical University

    PRINCIPAL INVESTIGATOR
  • Songfu Jiang, MD

    First Affiliated Hospital of Wenzhou Medical University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Head of Department of Hematology

Study Record Dates

First Submitted

September 26, 2017

First Posted

October 5, 2017

Study Start

December 29, 2017

Primary Completion

December 12, 2020

Study Completion

December 12, 2023

Last Updated

October 8, 2020

Record last verified: 2020-10

Data Sharing

IPD Sharing
Will not share

Locations