Anabolic Effects of Whey and Casein After Strength Training in Young and Elderly

NCT02968888 | Completed

• 1 other identifier: Tine acute
• Study Type: interventional
• Target: 43
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to investigate the acute anabolic effects of native whey, whey protein concentrate 80 (WPC-80) and milk after a bout of strength training in young and elderly. The investigators hypothesize that native whey will give a greater stimulation of muscle protein synthesis and intracellular anabolic signaling than WPC-80, and that WPC-80 will give a stronger stimulus than milk.

Conditions

Healthy; Young; Elderly

Outcome Measures

Primary Outcomes (4)

• Mixed muscle fractional synthetic rate

  A continous infusion of a stable isotope (phe D5) is used to measure incorporation of tracer into muscle (biopsies from m. vastus lateralis)

  Three to one hours prior to a bout of strength training and protein consumption

• Mixed muscle fractional synthetic rate

  A continous infusion of a stable isotope (phe D5) is used to measure incorporation of tracer into muscle (biopsies from m. vastus lateralis)

  One to five hours after a bout of strength training and protein consumption

• Mixed muscle fractional synthetic rate

  Two boluses of tracer (phe13C6 and phe15N) was used to measure incorporation of tracer into muscle (biopsies from m. vastus lateralis)

  From three to five hours after a bout of strength training and protein consumption

• Mixed muscle fractional breakdown rate

  Two boluses of tracer (phe13C6 and phe15N) was used to measure the dilution of tracer in muscle (biopsies from m. vastus lateralis)

  From three to five hours after a bout of strength training and protein consumption

Secondary Outcomes (96)

• Ratio of phosphorylated to total ribosomal protein S6 kinase beta-1(P70S6K) change from baseline

  30 min before, 1, 2.5 and 5 hours after training and protein intake

• Phosphorylation of phosphorylated to total eukaryotic elongation factor 2 (eEF-2) change from baseline

  30 min before, 1, 2.5 and 5 hours after training and protein intake

• Phosphorylation of phosphorylated to total eukaryotic translation initiation factor 4E-binding protein 1 (4EBP-1) change from baseline

  30 min before, 1, 2.5 and 5 hours after training and protein intake

• Intracellular translocation of forkhead box O3 (FOXO3a) change from baseline

  30 min before, 1, 2.5 and 5 hours after training and protein intake

• Intracellular translocation of muscle RING-finger protein-1 (Murf-1) change from baseline

  30 min before, 1, 2.5 and 5 hours after training and protein intake

Study Arms (3)

Milk

PLACEBO COMPARATOR

Participants performed a bout of strength training and consumed 20g of milk protein

Other: Strength training; Dietary Supplement: Milk 1%

Whey protein concentrate 80

EXPERIMENTAL

Participants performed a bout of strength training and consumed 20g of whey protein concentrate 80

Other: Strength training; Dietary Supplement: Whey protein concentrate 80

Native whey

EXPERIMENTAL

Participants performed a bout of strength training and consumed 20g of native whey

Other: Strength training; Dietary Supplement: Native whey

Interventions

Strength training OTHER

Milk; Native whey; Whey protein concentrate 80

Milk 1% DIETARY_SUPPLEMENT

Milk

Whey protein concentrate 80 DIETARY_SUPPLEMENT

Whey protein concentrate 80

Native whey DIETARY_SUPPLEMENT

Native whey

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Healthy in the sense that they can conduct training and testing
• Able to understand Norwegian language written and oral
• Between 18 and 45, or above 70 years of age

You may not qualify if:

• Diseases or injuries contraindicating participation
• Use of dietary supplements (e.g. proteins, vitamins and creatine)
• Lactose intolerance
• Allergy to milk
• Allergy towards local anesthetics (xylocain)

Sponsors & Collaborators

• Norwegian School of Sport Sciences: lead
• Tine: collaborator
• The Research Council of Norway: collaborator
• Arkansas Children's Hospital Research Institute: collaborator

Study Sites (1)

Norwegian School of Sport Sciences

Oslo, 0863, Norway

Location

Related Publications (1)

• Hamarsland H, Nordengen AL, Nyvik Aas S, Holte K, Garthe I, Paulsen G, Cotter M, Borsheim E, Benestad HB, Raastad T. Native whey protein with high levels of leucine results in similar post-exercise muscular anabolic responses as regular whey protein: a randomized controlled trial. J Int Soc Sports Nutr. 2017 Nov 21;14:43. doi: 10.1186/s12970-017-0202-y. eCollection 2017.

  PMID: 29200982 DERIVED

MeSH Terms

Interventions

Resistance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD student

Study Record Dates

• First Submitted: November 10, 2016
• First Posted: November 21, 2016
• Study Start: August 1, 2014
• Primary Completion: April 1, 2015
• Study Completion: May 1, 2017
• Last Updated: April 10, 2018

Record last verified: 2018-04

Locations

NO (1)