Nutritional Strategies to Augment the Postprandial Muscle Protein Synthetic Response to the Ingestion of a Low Dose of Protein in Middle-aged Women

NCT02918981 | Completed DMC

• 1 other identifier: 16999
• Study Type: interventional
• Target: 50
• Locations: 1 country
• Sites: 1

Brief Summary

Using stable isotope methodology, investigators will determine the postabsorptive and postprandial muscle protein synthetic response in 60 female adults (Age 50-79, BMI: \<30 kg/m2) immediately after an acute bout of resistance exercise. Participants will be divided in to 5 groups of twelve women (n=12). Each group will receive a different protein beverage or placebo to consume following the resistance exercise training. Group 1 will consume a beverage consisting of whey protein; group 2 will consume whey protein supplemented with additional leucine; group 3 will consume whey protein supplemented with additional leucine and hydrolyzed whey peptides; group 4 will consume whey protein supplemented with additional leucine and citrulline; group 5 will just consume water. During the testing, blood and muscle samples will be collected.

Conditions

Aging

Outcome Measures

Primary Outcomes (1)

• The myofibrillar protein synthetic response to protein ingestion

  Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 4 hr after the ingestion of the experimental beverages. This will allow us to assess the change from the postabsorptive to the postprandial period

  Postabsorptive for 3 hours, Postprandial for 4 hours hours

Secondary Outcomes (1)

• mTORC1 phosphorylation

  Baseline and at 2 and 4 hours after protein ingestion

Study Arms (5)

Whey protein

ACTIVE COMPARATOR

After resistance exercise, participants aged 50-79 years will consume 14g Whey protein dissolved in 200 mL of water

Behavioral: Resistance Exercise; Dietary Supplement: 14 g Whey protein

Whey + Leucine

EXPERIMENTAL

After resistance exercise, participants aged 50-79 years will consume 6.6 g Whey protein + 1.25 g Leucine dissolved in 200 mL of water

Behavioral: Resistance Exercise; Dietary Supplement: 6.6 g Whey protein + 1.25 g leucine

Whey + Leucine + Whey peptides

EXPERIMENTAL

After resistance exercise, participants aged 50-79 years will consume 4 g Whey protein + 1.25 g Leucine + 2.6 g Whey peptides dissolved in 200 mL of water

Behavioral: Resistance Exercise; Dietary Supplement: 4 g Whey protein + 1.25 g leucine + 2.6 g Whey peptides

Whey + Leucine + Citrulline

EXPERIMENTAL

After resistance exercise, participants aged 50-79 years will consume 6.6 g Whey protein + 1.25 g Leucine + 0.8 g Citrulline dissolved in 200 mL of water

Behavioral: Resistance Exercise; Dietary Supplement: 6.6 g Whey protein + 1.25 g leucine + 0.8 g Citrulline

Water

SHAM COMPARATOR

After resistance exercise, participants aged either 20-30 or 50-79 years old will consume 200 mL water

Behavioral: Resistance Exercise; Other: Water control

Interventions

Resistance Exercise BEHAVIORAL

Participants will perform unilateral leg extension exercise immediately prior to ingestion of the experimental beverage

Water; Whey + Leucine; Whey + Leucine + Citrulline; Whey + Leucine + Whey peptides; Whey protein

14 g Whey protein DIETARY_SUPPLEMENT

Whey protein

6.6 g Whey protein + 1.25 g leucine DIETARY_SUPPLEMENT

Whey + Leucine

4 g Whey protein + 1.25 g leucine + 2.6 g Whey peptides DIETARY_SUPPLEMENT

Whey + Leucine + Whey peptides

6.6 g Whey protein + 1.25 g leucine + 0.8 g Citrulline DIETARY_SUPPLEMENT

Whey + Leucine + Citrulline

Water control OTHER

Water

Eligibility Criteria

• Age: 20 Years - 79 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• BMI \<30 kg/m2
• Healthy, sedentary
• Either 20-30 years old or 50-79 years old

You may not qualify if:

• Tobacco use
• Allergies to milk consumption
• Unusually high protein consumption
• Vegan diet
• Allergies to antibiotics or xylocaine
• Phenylketonuria (PKU)
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Heart disease
• Metabolic disorders
• Liver, kidney, or urinary disease
• Musculoskeletal problems
• Autoimmune disease
• Neurological disease

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead

Study Sites (1)

Freer Hall

Urbana, Illinois, 61801, United States

Location

MeSH Terms

Interventions

Resistance Training; Whey Proteins; Leucine; Citrulline

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Milk Proteins; Animal Proteins, Dietary; Dietary Proteins; Proteins; Amino Acids, Peptides, and Proteins; Whey; Milk; Dairy Products; Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages; Amino Acids, Branched-Chain; Amino Acids; Amino Acids, Essential; Amino Acids, Diamino

Study Officials

• Nicholas A Burd, Ph.D.

  University of Illinois at Chicago

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: September 20, 2016
• First Posted: September 29, 2016
• Study Start: October 1, 2016
• Primary Completion: March 6, 2019
• Study Completion: March 6, 2019
• Last Updated: November 3, 2020

Record last verified: 2020-11

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)