Muscle Protein Synthesis Rates After Protein Consumption in Lean, Overweight, and Obese Adults

NCT02613767 | Completed

• 1 other identifier: 15161
• Study Type: observational
• Target: 32
• Locations: 0 countries
• Sites: N/A

Brief Summary

There are an increasing number of individuals in the United States with obesity, and this is a major health concern with links to many chronic diseases. Impairments in protein metabolism with obesity may disrupt muscle function and modify the dietary protein requirements in obese individuals. Further, overweight and obese type 2 diabetics exhibit dramatically reduced skeletal muscle mass compared with lean, healthy controls. Surprisingly, the influence of being overweight or obese on this decline in muscle mass remains understudied, despite clear evidence that similar metabolic impairments typically exist in these populations prior to the development of overt diabetes. Protein ingestion provides the amino acid building blocks to synthesize and repair muscle proteins in adults. Previous research has shown that the muscle protein synthetic response to food ingestion may be reduced in overweight/obese adults. However, this research provided the 'free' amino acids in small portions every 15 min during the postprandial period. In free living conditions, however, it is more common to consume protein dense foods in single portions. Currently, there is no information available on how eating protein rich foods affects muscle protein synthesis in overweight and obese adults. This proposed research will fill this research gap by being the first study to compare the muscle protein synthetic response to the ingestion of a meal-like amount of high quality protein in lean, overweight and obese adults. The objective of this study is to determine the muscle protein synthetic response after the consumption of 35g pork protein in lean, overweight and obese adults. In order to assess this objective the researchers propose to use primed continuous infusion of L-\[ring-13C6\] phenylalanine to measure muscle protein synthesis rates after the consumption of dietary protein. In a parallel design the researchers will study 13 obese (BMI 30-39.9 kg/m2) participants, 13 overweight (BMI 25-29.9 kg/m2), and 13 age-matched lean controls (BMI 18-24.9 kg/m2) between the ages of 20 and 45 years. All subjects will be sedentary and weight stable for the previous 6 months. On the test day, subjects will remain sedentary for the determination of muscle protein synthesis in both the fasted state and after consumption of the protein meal. Blood and muscle sampling will occur on the test day.

Conditions

Obesity

Outcome Measures

Primary Outcomes (1)

• Fractional synthetic rate of myofibrillar proteins

  7 hours

Study Arms (3)

Obese

BMI \>30 kg/m2, Protein meal, L-\[ring 13C6\]Phenylalanine infusion

Other: Protein meal; Other: L-[ring 13C6]Phenylalanine infusion

Overweight

BMI \>25 and \< 30 kg/m2, Protein meal, L-\[ring 13C6\]Phenylalanine infusion

Other: Protein meal; Other: L-[ring 13C6]Phenylalanine infusion

Healthy-Weight

BMI \>18 and \< 25 kg/m2, Protein meal, L-\[ring 13C6\]Phenylalanine infusion

Other: Protein meal; Other: L-[ring 13C6]Phenylalanine infusion

Interventions

Protein meal OTHER

Ingestion of protein

Healthy-Weight; Obese; Overweight

L-[ring 13C6]Phenylalanine infusion OTHER

experimental method for determining protein synthesis rates

Healthy-Weight; Obese; Overweight

Eligibility Criteria

• Age: 20 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

We recruited 32 healthy, sedentary adult subjects (20-45 y) for this investigation. There are three groups being studied, which will be stratified by body mass index. Because randomization is not possible, groups were balanced by age and sex. No races or ethnic groups will be specifically recruited or excluded.

You may qualify if:

• Males and Females
• Aged between 20-45 years
• Healthy, sedentary
• Three groups, age and sex matched
• Healthy weight group: BMI 18-24.9 kg/m2
• Overweight group: BMI 25-29.9 kg/m2
• Obese group: BMI 30-39.9 kg/m2

You may not qualify if:

• Smoking
• Allergies to pork consumption
• Unusually high protein consumption
• Vegetarians
• Phenylketonuria (PKU)
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Previous participation in amino acid tracer studies
• Predisposition to hypertrophic scarring or keloid formation
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
• Irregular menstrual cycles during the previous year
• Pregnancy
• High BMI that is not representative of being overweight or obese (e.g. resistance trained individuals, football players)

Sponsors & Collaborators

• University of Illinois at Urbana-Champaign: lead

Related Publications (2)

• Beals JW, Mackenzie RWA, van Vliet S, Skinner SK, Pagni BA, Niemiro GM, Ulanov AV, Li Z, Dilger AC, Paluska SA, De Lisio M, Burd NA. Protein-Rich Food Ingestion Stimulates Mitochondrial Protein Synthesis in Sedentary Young Adults of Different BMIs. J Clin Endocrinol Metab. 2017 Sep 1;102(9):3415-3424. doi: 10.1210/jc.2017-00360.

  PMID: 28911136 DERIVED

• Beals JW, Sukiennik RA, Nallabelli J, Emmons RS, van Vliet S, Young JR, Ulanov AV, Li Z, Paluska SA, De Lisio M, Burd NA. Anabolic sensitivity of postprandial muscle protein synthesis to the ingestion of a protein-dense food is reduced in overweight and obese young adults. Am J Clin Nutr. 2016 Oct;104(4):1014-1022. doi: 10.3945/ajcn.116.130385. Epub 2016 Sep 7.

  PMID: 27604771 DERIVED

Biospecimen

Retention: SAMPLES WITHOUT DNA

Plasma samples and Skeletal muscle tissue

MeSH Terms

Conditions

Obesity

Interventions

guar meal protein, Cyamopsis tetragonoloba

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Officials

• Nicholas A Burd, Ph.D

  Assistant Professor

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: November 5, 2015
• First Posted: November 24, 2015
• Study Start: December 1, 2014
• Primary Completion: September 1, 2015
• Study Completion: August 1, 2016
• Last Updated: October 26, 2016

Record last verified: 2016-10