NCT02478775

Brief Summary

Excess maternal weight, especially obesity, influences almost every aspect of fertility, from conception to problems during pregnancy. The investigators will use novel statistical methods to clarify the hormonal changes behind reproductive health conditions. A better understanding of reproductive hormonal changes in obese women may offer a way to identify new treatments.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
99

participants targeted

Target at P50-P75 for not_applicable obesity

Timeline
Completed

Started Jul 2015

Longer than P75 for not_applicable obesity

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 7, 2015

Completed
2 months until next milestone

First Posted

Study publicly available on registry

June 23, 2015

Completed
8 days until next milestone

Study Start

First participant enrolled

July 1, 2015

Completed
6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 30, 2021

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 30, 2021

Completed
9 months until next milestone

Results Posted

Study results publicly available

April 7, 2022

Completed
Last Updated

June 5, 2024

Status Verified

May 1, 2024

Enrollment Period

6 years

First QC Date

May 7, 2015

Results QC Date

January 12, 2022

Last Update Submit

May 10, 2024

Conditions

Keywords

obesityfertilityinfertilityreproductive hormones

Outcome Measures

Primary Outcomes (1)

  • Difference Between Peak Inhibin B

    This is defined as the maximum hormone value during Day 1 of the study subtracted from the maximum hormone value during Day 2.

    Every 10 minutes over 2 10-hour frequent blood sampling sessions.

Secondary Outcomes (2)

  • Peak Inhibin B Per Subject

    Every 10 minutes over 10 hours on Day 1 and Day 2 of the study.

  • Peak E2 Per Subject

    Every 10 minutes over 10 hours of Day 1 and Day 2 of the study.

Study Arms (2)

Frequent Blood Sampling, Degarelix

EXPERIMENTAL

Investigators will assess the change in inhibin B levels following repeated bolus dosing of recombinant FSH (rFHS) following Degarelix (GnRH antagonist) blockade over a 2-day period. This arm was terminated due to Adverse Events and the study was continued with the Cetrorelix product.

Drug: Degarelix (GnRH antagonist)Drug: recombinant FSH

Frequent Blood Sampling, Cetrorelix

EXPERIMENTAL

Investigators will assess the change in inhibin B levels following repeated bolus dosing of recombinant FSH (rFHS) following Cetrorelix blockade over a 2-day period.

Drug: recombinant FSHDrug: Cetrorelix

Interventions

Day-1: Blood samples will be obtained every 10 minutes for 10 hours. After 10 hours of blood samples have been collected, the GnRH antagonist degarelix will be given subcutaneously.

Also known as: Firmagon
Frequent Blood Sampling, Degarelix

Day-2: Blood samples will again be obtained every 10 minutes for 10 hours. Repeated boluses of exogenous recombinant FSH (rFSH) will be given by IV during this 10 hour visit.

Also known as: rFHS
Frequent Blood Sampling, CetrorelixFrequent Blood Sampling, Degarelix

Day-1: Blood samples will be obtained every 10 minutes for 10 hours. After 10 hours of blood samples have been collected, the GnRH antagonist Cetrorelix will be given subcutaneously.

Also known as: Cetrotide
Frequent Blood Sampling, Cetrorelix

Eligibility Criteria

Age21 Years - 39 Years
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Age between 21 to 39 years old with regular menstrual cycles every 25-40 days
  • Body mass of 18.5 kg/m2-24.9kg/m2 (normal weight controls) or greater than 30.0 kg/m2 (obese group)
  • Prolactin and thyroid-stimulating hormone (TSH) within normal laboratory ranges at screening
  • Baseline hemoglobin \>11 gm/dl.

You may not qualify if:

  • Diagnosis of polycystic ovary syndrome (PCOS), defined by the 2003 Rotterdam criteria as suggested by 2012 NIH Workshop
  • History of chronic disease affecting hormone production, metabolism or clearance or use of thiazolidinediones or metformin (known to interact with reproductive hormones)
  • Use of hormones affecting hypothalamic-pituitary-gonadal (HPO) axis (such as hormonal contraceptives) within 3 months of entry
  • Strenuous exercise (\>4 hours of intense physical activity per week)
  • Pregnancy
  • Breast-feeding
  • Current attempts to conceive
  • Significant recent weight loss or gain

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Colorado Clinical and Translational Research Center

Aurora, Colorado, 80045, United States

Location

Related Publications (1)

  • Luu TH, Kuhn K, Bradford AP, Wempe MF, Wittenburg L, Johnson RL, Carlson NE, Kumar TR, Polotsky AJ. Effects of pulsatile intravenous follicle-stimulating hormone treatment on ovarian function in women with obesity. Fertil Steril. 2023 Oct;120(4):890-898. doi: 10.1016/j.fertnstert.2023.05.170. Epub 2023 Jun 3.

MeSH Terms

Conditions

ObesityInfertility

Interventions

acetyl-2-naphthylalanyl-3-chlorophenylalanyl-1-oxohexadecyl-seryl-4-aminophenylalanyl(hydroorotyl)-4-aminophenylalanyl(carbamoyl)-leucyl-ILys-prolyl-alaninamidecetrorelix

Condition Hierarchy (Ancestors)

OverweightOvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsGenital DiseasesUrogenital Diseases

Limitations and Caveats

The Degarelix arm is excluded from outcome measure data because no usable data could be collected from those normal weight participants. There were no participants in this arm that were of high BMI. The Degarelix arm was terminated due to adverse events and was replaced with the Cetrorelix arm.

Results Point of Contact

Title
Dr. Alex Polotsky
Organization
University of Colorado School of Medicine

Study Officials

  • Alex Polotsky, MD

    University of Colorado, Denver

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 7, 2015

First Posted

June 23, 2015

Study Start

July 1, 2015

Primary Completion

June 30, 2021

Study Completion

June 30, 2021

Last Updated

June 5, 2024

Results First Posted

April 7, 2022

Record last verified: 2024-05

Data Sharing

IPD Sharing
Will not share

Locations