NCT02431988

Brief Summary

The purpose of this study is to administer novel cluster of differentiation antigen 19 (CD19) specific Chimeric Antigen Receptor T-cells (CAR19 T-cells) to patients with relapsed or resistant Diffuse Large B Cell Lymphoma (DLBCL) to assess the safety and efficacy of this strategy as a bridge to allogeneic transplantation.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
10

participants targeted

Target at below P25 for phase_1

Timeline
Completed

Started Jun 2016

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 23, 2015

Completed
1 month until next milestone

First Posted

Study publicly available on registry

May 1, 2015

Completed
1.1 years until next milestone

Study Start

First participant enrolled

June 1, 2016

Completed
3.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 2, 2020

Completed
2.1 years until next milestone

Study Completion

Last participant's last visit for all outcomes

March 21, 2022

Completed
Last Updated

April 28, 2023

Status Verified

April 1, 2023

Enrollment Period

3.7 years

First QC Date

March 23, 2015

Last Update Submit

April 26, 2023

Conditions

Keywords

RelapsedrefractoryDLBCL

Outcome Measures

Primary Outcomes (3)

  • Feasibility of adequate leucapheresis collection and generation of CAR19 T cells.

    The number of CAR19 T cells successfully manufactured as a fraction of the number of patients undergoing leukapheresis (all patients registered).

    1 month

  • Toxicity evaluation following CAR19 T-cell administration.

    Toxicity will be examined for each patient receiving CAR19 T cells, using the maximum grade for each toxicity type, all summarized as number of patients with adverse events.

    1 year

  • Efficacy of CAR19 T-cells.

    Efficacy will be defined as the number of patients that meet the clinical complete responders criteria.

    1 year

Secondary Outcomes (5)

  • CAR19 T-cell engraftment

    1 year

  • B cell compartment

    1 year

  • Cytokine profile

    1 year

  • Clinical complete response

    1 month

  • Eligibility to allogeneic transplantation

    1-3 years

Study Arms (1)

CAR19 T-cells

EXPERIMENTAL

Patients will receive a single infusion of CAR19 T-cells following standard pre-conditioning with cyclophosphamide and fludarabine. The CAR19 T-cells are to be administered on day 0.

Procedure: LeukapheresisDrug: CyclophosphamideDrug: FludarabineBiological: CAR19 T-Cells

Interventions

LeukapheresisPROCEDURE

Patients will undergo leukapheresis prior to pre-conditioning chemotherapy to provide the immune cells required to produce the therapeutic product.

CAR19 T-cells

Patients will receive a standard pre-conditioning regime with cyclophosphamide 60mg/kg/day IV over 1 hour for 2 days (day-7 and day-6).

CAR19 T-cells

Fludarabine 25mg/m2/day IV over 15/30 minutes for 5 days (Day-5 to day-1).

CAR19 T-cells
CAR19 T-CellsBIOLOGICAL

The CAR19 T-cells are to be administered on day 0 at the dose specified by the Cancer Trials Centre (CTC) at the time of registration. Three dose cohorts are planned: * Dose Level 1: 2x105 CAR19 T-cells/kg * Dose Level 2: 1x106 CAR19 T-cells/kg * Dose Level 3: 5x106 CAR19 T-cells/kg

Also known as: CD19 specific Chimeric Antigen Receptor T-cells
CAR19 T-cells

Eligibility Criteria

Age16 Years - 65 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Age 16-65 years
  • Confirmed diagnosis of CD19+ DLBCL
  • Primary resistant or relapsed disease failing to achieve metabolic Complete Response (CR) to 1st line salvage, or relapse post autograft failing to achieve metabolic CR following a single further cycle of salvage
  • Potential allogeneic transplant candidate
  • Agreement to have a pregnancy test, use adequate contraception for 12 months post-CAR19 T-cell infusion
  • Karnofsky performance status \>60
  • Written informed consent

You may not qualify if:

  • Women who are pregnant or lactating
  • Prior allogeneic transplantation
  • Progressive disease following most recent salvage prior to planned leucapheresis (those with mixed response are eligible)
  • Prior history of ischaemic heart disease, dysrhythmias, abnormal electrocardiogram (ECG)(Left Bundle Branch Block (LBBB)), Multiple Gated Acquisition (MUGA) left ventricular ejection fraction (LVEF) \<40%
  • Known central nervous system (CNS) involvement or cerebral vascular accident (CVA) within prior 3 months
  • Patients receiving corticosteroids at a dose of \> 10mg prednisolone per day (or equivalent)
  • Use of rituximab within the last 2 months prior to CAR19 T-cell infusion
  • Active autoimmune disease requiring immunosuppression
  • Life expectancy \<3 months
  • Known allergy to albumin or dimethylsulfoxide (DMSO)
  • Any contraindication to the administration and use of ifosfamide, epirubicin, etoposide, fludarabine and cyclophosphamide.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University College London Hospital

London, United Kingdom

Location

Related Publications (1)

  • Ernst M, Oeser A, Besiroglu B, Caro-Valenzuela J, Abd El Aziz M, Monsef I, Borchmann P, Estcourt LJ, Skoetz N, Goldkuhle M. Chimeric antigen receptor (CAR) T-cell therapy for people with relapsed or refractory diffuse large B-cell lymphoma. Cochrane Database Syst Rev. 2021 Sep 13;9(9):CD013365. doi: 10.1002/14651858.CD013365.pub2.

MeSH Terms

Conditions

Lymphoma, Large B-Cell, DiffuseRecurrence

Interventions

LeukapheresisCyclophosphamidefludarabine

Condition Hierarchy (Ancestors)

Lymphoma, B-CellLymphoma, Non-HodgkinLymphomaNeoplasms by Histologic TypeNeoplasmsLymphoproliferative DisordersLymphatic DiseasesHemic and Lymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesDisease AttributesPathologic ProcessesPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

CytapheresisBiological TherapyTherapeuticsBlood Component RemovalLeukocyte Reduction ProceduresCell SeparationCytological TechniquesClinical Laboratory TechniquesInvestigative TechniquesPhosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus Compounds

Study Officials

  • Karl Peggs

    University College, London

    STUDY CHAIR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NA
Masking
NONE
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 23, 2015

First Posted

May 1, 2015

Study Start

June 1, 2016

Primary Completion

February 2, 2020

Study Completion

March 21, 2022

Last Updated

April 28, 2023

Record last verified: 2023-04

Locations