Bioavailability of Carotenoids and Flavonoids From Fresh Oranges and Orange Juice.

NCT02380144 | Unknown

• 1 other identifier: F-2013-097
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

The study was designed as a randomized 2-way cross-over study to assess the bioavailability of carotenoids and flavonoids from sweet oranges and orange juice. Volunteers followed a wash-out period for 2 weeks prior to eating either 400 g of fresh oranges or 719 g of orange juice. The amount of test food was adjusted to deliver an equal amount of β-cryptoxanthin (740 µg). After a consecutive wash-out period of 2 weeks, the procedure was repeated and the volunteers ingested the complementary test food. Blood samples were collected at 8 time points up to 9.5 h after test meal consumption. Additionally, 24 h-urine was collected to evaluate flavonoid recovery.

Conditions

Healthy

Keywords

carotenoid; bioavailability; beta-cryptoxanthin; flavonoid; hesperidin; narirutin; randomized cross-over; Pharmacokinetics; micronutrients; Oranges/Orange Juice

Outcome Measures

Primary Outcomes (1)

• Mean AUC (nmol/L*9.5 h) of beta-cryptoxanthin concentration in blood plasma chylomicrons after ingestion of fresh oranges and orange juice, respectively.

  0 - 9.5 h after each intervention

Secondary Outcomes (2)

• Mean AUC of hesperidin- and narirutin-metabolite concentration in blood plasma after ingestion of fresh oranges and orange juice, respectively.

  0 - 9.5 h after each intervention

• Mean hesperetin and naringenin concentration (µmol/L) in 24 h-urine after ingestion of fresh oranges and orange juice, respectively.

  0 - 24 h after each intervention

Study Arms (2)

Orange Fruit - Orange Juice

EXPERIMENTAL

Sequence of test foods during the intervention period: 1st: Orange fruit; 2nd: Orange juice

Other: Fresh orange fruit and pasteurized fruit juice

Orange Juice - Orange Fruit

EXPERIMENTAL

Sequence of test foods during the intervention period: 1st: Orange juice; 2nd: Orange fruit

Other: Fresh orange fruit and pasteurized fruit juice

Interventions

Fresh orange fruit and pasteurized fruit juice OTHER

Orange Fruit - Orange Juice; Orange Juice - Orange Fruit

Eligibility Criteria

• Age: 23 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy adult men and women
• BMI: 17-60
• Age: 18-65

You may not qualify if:

• Women who are lactating or pregnant or plan to be pregnant
• Individuals using tobacco products
• Individuals with metabolic or malabsorption disorders
• Individuals with a history of cancer
• Individuals with a history of liver insufficiency
• Individuals with a history of chronic and lipid metabolism-related diseases
• Individuals with an allergy to oranges
• Obese individuals (BMI \> 30)

Sponsors & Collaborators

• University of Hohenheim: lead

Study Sites (1)

Chair of Plant Foodstuff Technology and Analysis (150d), University of Hohenheim

Stuttgart, Baden-Wurttemberg, 70599, Germany

Location

Related Publications (1)

• Aschoff JK, Rolke CL, Breusing N, Bosy-Westphal A, Hogel J, Carle R, Schweiggert RM. Bioavailability of beta-cryptoxanthin is greater from pasteurized orange juice than from fresh oranges - a randomized cross-over study. Mol Nutr Food Res. 2015 Oct;59(10):1896-904. doi: 10.1002/mnfr.201500327. Epub 2015 Jul 20.

  PMID: 26114420 RESULT

Study Officials

• Reinhold Carle, Prof. Dr.

  University of Hohenheim

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 23, 2015
• First Posted: March 5, 2015
• Study Start: January 1, 2014
• Primary Completion: May 1, 2014
• Study Completion: July 1, 2017
• Last Updated: November 1, 2016

Record last verified: 2016-10

Locations

DE (1)