NCT02359240|UnknownDMC
HO-1 and Muscle Mitochondrial Dysfunction in Sepsis
Skeletal Muscle Wasting in Patients With Sepsis
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Skeletal Muscle Wasting in Patients With Sepsis
Sponsor study identifier
observational
50
1 country
1
Study Start
December 2014
Date type not reportedThis date has not been verified as actual or estimated.
Primary Completion
June 2016
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Study Completion
August 2016
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
The current project was designed to examine dynamic changes in muscle wasting during sepsis. Researchers will focus the mitochondrial dysfunction of muscle cells and investigate the role of HO-1 in it. Researchers interested in identifying factors involved in the pathology of muscle wasting during sepsis.
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
August 2016
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
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Some registry-reported names shown below are not yet linked in this graph.
Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
December 2014
Date type not reportedThis date has not been verified as actual or estimated.
January 28, 2015
Actual (reported)February 9, 2015
Actual (reported)June 2016
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
August 2016
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
HO-1 mRNA and protein expression, mitochondria function of musculus vastus lateralis
3 days after diagnosis
length of ICU stay
60 days
length of mechanical ventilation
60 days
muscle myosin content
3 days after diagnosis
Patients with a diagnosis of sepsis or severe sepsis,immobilization for 3 days at least. Therapy according to the SSC guidelines will be applied. therapy according to SSC guidelines and muscle biopsy
non septic patients with a femoral fracture,who need an fixation surgery.standard surgery for femoral fracture and muscle biopsy will be performed.
Septic patients will be treated according to the international guidelines for management of severe sepsis and septic shock 2012. Skeletal muscle biopsies, blotted free from blood, are frozen within 30 seconds in liquid nitrogen, and stored in liquid nitrogen until processing.150-300 mg muscle (wet weight) was routinely obtained , which is more than sufficient for the analyses proposed, with some muscle remaining for any additional validation assays that may arise during the course of the research. Muscle will be processed either for isolation of mRNA/microRNA or protein
septic patients; non septic patients with a femoral fracture,who need an fixation surgery
Anesthesiology and Intensive Care, Changhai Hospital
Shanghai, Shanghai Municipality, 200433, China
skeletal muscle
Xiaoming Deng, M.D., Ph.D.
Changhai Hospital
First Submitted
January 28, 2015
First Posted
February 9, 2015
Study Start
December 2014
Date type not reportedThis date has not been verified as actual or estimated.
Primary Completion
June 2016
Effects of Subanesthetic Isoflurane/Sevoflurane in 60% Oxygen on Clinical In-vitro Experimental Sepsis
Patients with femoral fracture will under standard surgery for fracture fixation. Percutaneous needle muscle biopsies will be performed. Skeletal muscle biopsies, blotted free from blood, are frozen within 30 seconds in liquid nitrogen, and stored in liquid nitrogen until processing.150-300 mg muscle (wet weight) was routinely obtained , which is more than sufficient for the analyses proposed, with some muscle remaining for any additional validation assays that may arise during the course of the research. Muscle will be processed either for isolation of mRNA/microRNA or protein
Registry-reported names are shown in full. Names without a link have not yet been matched to a Syfrah profile.
Contact the study team to confirm eligibility.
This estimate has passed. The registry has not confirmed an actual date for this milestone.
Study Completion
August 2016
Estimated (date passed)This estimate has passed. The registry has not confirmed an actual date for this milestone.
Last Updated
February 9, 2015
Record last verified: 2015-02
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