NCT02287311

Brief Summary

The subject has a type of cancer or lymph gland disease associated with a virus called Epstein Barr Virus (EBV), which has come back, is at risk of coming back, or has not gone away after standard treatments. This research study uses special immune system cells called LMP, BARF-1 and EBNA1- specific cytotoxic T lymphocytes (MABEL CTLs). Some patients with Lymphoma (such as Hodgkin (HD) or non-Hodgkin Lymphoma (NHL)), T/NK-lymphoproliferative disease, or CAEBV, or solid tumors such as nasopharyngeal carcinoma (NPC), smooth muscle tumors, and leiomyosarcomas show signs of a virus called EBV before or at the time of their diagnosis. EBV causes mononucleosis or glandular fever ("mono" or the "kissing disease"). EBV is found in the cancer cells of up to half the patients with HD and NHL, suggesting that it may play a role in causing Lymphoma. The cancer cells (in lymphoma) and some immune system cells (in CAEBV) infected by EBV are able to hide from the body's immune system and escape destruction. EBV is also found in the majority of NPC and smooth muscle tumors, and some leiomyosarcomas. Investigators want to see if special white blood cells (MABEL CTLs) that have been trained to kill EBV infected cells can survive in patients blood and affect the tumor. In previous studies, EBV CTLs were generated from the blood of the patient, which was often difficult if the patient had recently received chemotherapy. Also, it took up to 1-2 months to make the cells, which is not practical when a patient needs more urgent treatment. To address these issues, the MABEL CTLs were made in the lab in a simpler, faster, and safer way. The MABEL CTLs will still see LMP proteins but also two other EBV proteins called EBNA-1 and BARF. To ensure these cells are available for use in patients in urgent clinical need, investigators have generated MABEL CTLs from the blood of healthy donors and created a bank of these cells, which are frozen until ready for use. Investigators have previously successfully used frozen T cells from healthy donors to treat EBV lymphoma and virus infections and we now have improved our production method to make it faster. In this study, investigators want to find out if they can use banked MABEL CTLs to treat HD, NHL, T/NK-lymphoproliferative disease, CAEBV, NPC, smooth muscle tumors or leiomyosarcoma. Investigators will search the bank to find a MABEL CTL line that is a partial match with the subject. MABEL CTLs are investigational and not approved by the Food and Drug Administration.

Trial Health

75
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
38

participants targeted

Target at P50-P75 for phase_1

Timeline
34mo left

Started Feb 2015

Longer than P75 for phase_1

Geographic Reach
1 country

2 active sites

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress80%
Feb 2015Mar 2029

First Submitted

Initial submission to the registry

November 6, 2014

Completed
4 days until next milestone

First Posted

Study publicly available on registry

November 10, 2014

Completed
3 months until next milestone

Study Start

First participant enrolled

February 1, 2015

Completed
9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 12, 2024

Completed
1.1 years until next milestone

Results Posted

Study results publicly available

February 24, 2025

Completed
4 years until next milestone

Study Completion

Last participant's last visit for all outcomes

March 1, 2029

Expected
Last Updated

March 23, 2026

Status Verified

March 1, 2026

Enrollment Period

9 years

First QC Date

November 6, 2014

Results QC Date

January 8, 2025

Last Update Submit

March 3, 2026

Conditions

Keywords

EBV positive diseasescytotoxic T lymphocytes

Outcome Measures

Primary Outcomes (1)

  • Number of Participants With a Dose-limiting Toxicity (DLT)

    To evaluate the safety of administering escalating doses of banked allogeneic, partially HLA-matched rapid EBV specific T cells.

    8 weeks

Secondary Outcomes (1)

  • Percent of Patients Whose Best Response is Either Complete Remission or Partial Remission

    8 weeks

Study Arms (3)

Group A: MABEL CTLs

EXPERIMENTAL

Patients with 1st or subsequent relapse. Three different dosing schedules will be evaluated. Two to four patients will be evaluated on each dosing schedule. Each patient will receive 2 injections, 14 days apart. If the patient's level of circulating T cells is relatively high, s/he may require treatment with cyclophosphamide (Cytoxan) and Fludarabine before s/he receives MABEL CTLs.

Biological: MABEL CTLsDrug: CyclophosphamideDrug: Fludarabine

Group B: MABEL CTLs

EXPERIMENTAL

Patients with persistent active disease despite therapy. Three different dosing schedules will be evaluated. Two to four patients will be evaluated on each dosing schedule. Each patient will receive 2 injections, 14 days apart. If the patient's level of circulating T cells is relatively high, s/he may require treatment with cyclophosphamide (Cytoxan) and Fludarabine before s/he receives MABEL CTLs.

Biological: MABEL CTLsDrug: CyclophosphamideDrug: Fludarabine

Group C: MABEL CTLs

EXPERIMENTAL

Patients with active disease if immunosuppressive chemotherapy is contraindicated. Three different dosing schedules will be evaluated. Two to four patients will be evaluated on each dosing schedule. Each patient will receive 2 injections, 14 days apart. If the patient's level of circulating T cells is relatively high, s/he may require treatment with cyclophosphamide (Cytoxan) and Fludarabine before s/he receives MABEL CTLs.

Biological: MABEL CTLsDrug: CyclophosphamideDrug: Fludarabine

Interventions

MABEL CTLsBIOLOGICAL

Dose escalation: DL1:2x10\^7 cells/m2+2x10\^7 cells/m2 DL2:2x10\^7cells/m2+5x10\^7 cells/m2 DL3:5x10\^7 cells/m2+1x10\^8 cells/m2 \*Doses are based on total CD3+cells/m2 Patients with active disease that have apparent clinical benefit at the 8 wk post 1st infusion (6 wks after 2nd infusion) or subsequent evaluations may receive up to 6 additional doses of CTLs at intervals at least 6 wks apart, each of which will consist of the same cell number as their second injection or below the original dose if there is not enough product available for the original dose. Patients may receive lymphodepleting chemotherapy (Cy/Flu) before additional infusions. Patients cannot receive additional doses until the initial safety profile is completed at 6 wks following the second infusion.

Also known as: LMP, BARF-1 and EBNA1 specific cytotoxic T lymphocytes, MABEL Cytotoxic T cells
Group A: MABEL CTLsGroup B: MABEL CTLsGroup C: MABEL CTLs

If the patient's level of circulating T cells is relatively high, s/he may require treatment with cyclophosphamide (Cytoxan) and Fludarabine before s/he receives MABEL CTLs. 3 daily doses of cyclophosphamide (Cy: 500 mg/m2/day) together with fludarabine (Flu: 30 mg/m2) to induce lymphopenia, finishing at least 24 hours before CTL infusion.

Also known as: Cytoxan
Group A: MABEL CTLsGroup B: MABEL CTLsGroup C: MABEL CTLs

If the patient's level of circulating T cells is relatively high, s/he may require treatment with cyclophosphamide and fludarabine before s/he receives MABEL CTLs. 3 daily doses of cyclophosphamide (Cy: 500 mg/m2/day) together with fludarabine (Flu: 30 mg/m2) to induce lymphopenia, finishing at least 24 hours before CTL infusion.

Also known as: Fludara
Group A: MABEL CTLsGroup B: MABEL CTLsGroup C: MABEL CTLs

Eligibility Criteria

Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • SCREENING
  • Any patient regardless of age or sex, with diagnosis of either:
  • EBV positive Hodgkin's lymphoma
  • EBV Positive non-Hodgkin's Lymphoma (regardless of histologic subtype)
  • EBV (associated)-T/NK-lymphoproliferative disease
  • Severe Chronic Active EBV (CAEBV) -- CAEBV is defined as patients with high EBV viral load in plasma or PBMC (\>4000 genomes per ug PBMC DNA) and/or biopsy tissue positive for EBV
  • Other EBV positive malignancies (e.g. nasopharyngeal carcinoma, smooth muscle tumors, etc.)
  • AND
  • in first or subsequent relapse (Group A)
  • with active disease persisting despite therapy (Group B)
  • with active disease if immunosuppressive chemotherapy is contraindicated e.g. patients who develop Hodgkin disease after solid organ transplantation or if the lymphoma is a second malignancy e.g. a Richter's transformation of CLL. (Group C)
  • EBV positive tumor
  • Weighs at least 12kg
  • Informed consent (and assent as applicable) obtained from patient/guardian.
  • TREATMENT
  • +21 more criteria

You may not qualify if:

  • TREATMENT
  • Pregnant or lactating
  • Severe intercurrent infection
  • Current use of systemic corticosteroids more than 0.5 mg/kg/day
  • Patients receiving ATG, Campath, or other immunosuppressive T cell monoclonal antibodies within 30 days.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Houston Methodist Hospital

Houston, Texas, 77030, United States

Location

Texas Children's Hospital

Houston, Texas, 77030, United States

Location

MeSH Terms

Conditions

Hodgkin DiseaseLymphoma, Non-HodgkinNasopharyngeal CarcinomaSmooth Muscle Tumor

Interventions

Cyclophosphamidefludarabinefludarabine phosphate

Condition Hierarchy (Ancestors)

LymphomaNeoplasms by Histologic TypeNeoplasmsLymphoproliferative DisordersLymphatic DiseasesHemic and Lymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesCarcinomaNeoplasms, Glandular and EpithelialNasopharyngeal NeoplasmsPharyngeal NeoplasmsOtorhinolaryngologic NeoplasmsHead and Neck NeoplasmsNeoplasms by SiteNasopharyngeal DiseasesPharyngeal DiseasesStomatognathic DiseasesOtorhinolaryngologic DiseasesNeoplasms, Muscle TissueNeoplasms, Connective and Soft Tissue

Intervention Hierarchy (Ancestors)

Phosphoramide MustardsNitrogen Mustard CompoundsMustard CompoundsHydrocarbons, HalogenatedHydrocarbonsOrganic ChemicalsPhosphoramidesOrganophosphorus Compounds

Results Point of Contact

Title
Dr. Rayne Rouce
Organization
Baylor College of Medicine

Study Officials

  • Rayne H Rouce, MD

    Baylor College of Medicine

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
Yes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant Professor

Study Record Dates

First Submitted

November 6, 2014

First Posted

November 10, 2014

Study Start

February 1, 2015

Primary Completion

January 12, 2024

Study Completion (Estimated)

March 1, 2029

Last Updated

March 23, 2026

Results First Posted

February 24, 2025

Record last verified: 2026-03

Locations