Study of Variation Over Time in the Oral Microbiome

Background: \- Normal bacteria and other tiny organisms are found in healthy human mouths. These are called oral microbiota. It is unclear exactly how the oral microbiota may affect health. For example, if the microbial composition is abnormal, it may lead to mouth conditions like periodontitis. Researchers want to study how the microbiota changes over time. This can help them plan future disease studies. Objectives: \- To see if and how oral microbiota change over time. Eligibility: \- Forty adult employees of the National Cancer Institute Shady Grove. Design:

• For 12 hours before the first visit, participants will not eat or drink (except water). They will not brush, floss, use mouthwash, chew gum, eat lozenges or candies, smoke cigarettes, or chew tobacco.
• At the first visit, participants will:
• Be given a saliva collector. They will spit 2 mL of saliva into it.
• Fill out an online questionnaire.
• Every 2 months, participants will visit the clinic and repeat visit 1.
• The study ends after 1 year. Sponsoring Institute: National Cancer Institute