Postprandial Muscle Protein Synthesis Following Wheat Protein Ingestion in Vivo in Humans

NCT01952639 | Completed

• 2 other identifiers: METC 13-3-045NL45958.068.13
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Rationale: The progressive loss of skeletal muscle mass with aging, or sarcopenia, has a major impact on our health care system due to increased morbidity and greater need for hospitalization and/or institutionalization. One way to prevent skeletal muscle loss is to improve dietary intake of the elderly. Both whey and casein seem to offer an anabolic advantage over soy protein for promoting muscle hypertrophy. As a consequence it is assumed that (all) plant based proteins have less potent anabolic properties when compared with animal based proteins. However, there is little theoretical background for such assumptions. Objective: To provide evidence for the efficacy of wheat protein and wheat protein hydrolysate when compared with milk proteins (i.e. whey and casein) as a dietary protein to stimulate postprandial muscle protein synthesis in vivo in healthy older humans. Study design: double-blind, placebo-controlled intervention study Study population: 60 healthy non-obese (BMI 18.5-30 kg/m2) older males (age: 65-80 y) Intervention: A protein beverage (350 mL) containing 30 g of whey, casein, wheat protein, or wheat protein hydrolysate or 60 g of wheat protein hydrolysate will be consumed (n=12 per group). Main study parameters/endpoints: Primary study parameters include muscle protein synthesis rates. Secondary study parameters include whole-body protein synthesis, breakdown, oxidation, and net balance. Hypotheses: We hypothesize that ingestion of wheat protein hydrolysate results in a greater muscle protein synthetic response when compared with the intact wheat protein due to its faster digestion and absorption. Furthermore, ingestion of wheat protein hydrolysate results in a higher muscle protein synthetic response when compared with casein, but lower when compared with whey protein. Ingestion of 60 g of wheat protein hydrolysate (amount of leucine equal to 30 g of whey protein) will result in a similar muscle protein synthetic response compared to ingestion of 30 g of whey protein.

Conditions

Sarcopenia

Outcome Measures

Primary Outcomes (1)

• muscle protein synthesis (MPS) rates

  Change in MPS from baseline after ingestion of different types of protein (baseline vs. postprandial period (0-5h))

Secondary Outcomes (1)

• whole-body protein metabolism

  Change in whole-body protein metabolism from baseline after ingestion of different types of protein (baseline vs. postprandial period (0-5h))

Study Arms (5)

30 g of wheat protein

EXPERIMENTAL

Subjects will consume 30 g of wheat protein protein type and amount

Dietary Supplement: protein type and amount

30 g of wheat protein hydrolysate

EXPERIMENTAL

Subjects will consume 30 g of wheat protein hydrolysate protein type and amount

Dietary Supplement: protein type and amount

30 g of whey protein

ACTIVE COMPARATOR

Subjects will consume 30 g of whey protein protein type and amount

Dietary Supplement: protein type and amount

30 g of casein

ACTIVE COMPARATOR

Subjects will consume 30 g of casein protein type and amount

Dietary Supplement: protein type and amount

60 g of wheat protein hydrolysate

EXPERIMENTAL

Subjects will consume 60 g of wheat protein hydrolysate protein type and amount

Dietary Supplement: protein type and amount

Interventions

protein type and amount DIETARY_SUPPLEMENT

Subjects will stay in a supine position and consume a test beverage containing different amounts ans types of protein

30 g of casein; 30 g of wheat protein; 30 g of wheat protein hydrolysate; 30 g of whey protein; 60 g of wheat protein hydrolysate

Eligibility Criteria

• Age: 65 Years - 80 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Older Adult (65+)

You may qualify if:

• Healthy males
• Age between 65 and 80 y
• BMI between 18.5 and 30 kg/m2

You may not qualify if:

• Wheat allergy
• Celiac disease
• Lactose intolerance
• Smoking
• Diabetes
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
• Use of anticoagulants
• Participation in exercise program
• Hypertension, high blood pressure that is above 140/90 mmHg.

Sponsors & Collaborators

• Maastricht University Medical Center: lead

Study Sites (1)

Maastricht University

Maastricht, Limburg, 6229 ER, Netherlands

Location

Related Publications (1)

• Gorissen SH, Horstman AM, Franssen R, Crombag JJ, Langer H, Bierau J, Respondek F, van Loon LJ. Ingestion of Wheat Protein Increases In Vivo Muscle Protein Synthesis Rates in Healthy Older Men in a Randomized Trial. J Nutr. 2016 Sep;146(9):1651-9. doi: 10.3945/jn.116.231340. Epub 2016 Jul 20.

  PMID: 27440260 DERIVED

MeSH Terms

Conditions

Sarcopenia

Condition Hierarchy (Ancestors)

Muscular Atrophy; Neuromuscular Manifestations; Neurologic Manifestations; Nervous System Diseases; Atrophy; Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms; Signs and Symptoms

Study Officials

• Luc JC van Loon, Prof. Dr.

  Maastricht University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: MSc

Study Record Dates

• First Submitted: September 23, 2013
• First Posted: September 30, 2013
• Study Start: January 1, 2014
• Primary Completion: October 1, 2014
• Study Completion: October 1, 2014
• Last Updated: December 2, 2014

Record last verified: 2014-11

Locations

NL (1)