Dietary Strategies to Promote Muscle Protein Anabolism in the Elderly

NCT00557388 | Completed DMC

• 2 other identifiers: MEC 07-3-086TIFN A-1002
• Study Type: interventional
• Target: 120
• Locations: 1 country
• Sites: 1

Brief Summary

Aging is associated with the loss of lean muscle mass, termed sarcopenia. Food intake and in particular the ingestion of protein or amino acids has been shown to be a powerful stimulus to promote net muscle protein anabolism. However this anabolic response following a meal-like protein bolus seems to be blunted in the elderly as compared to young adults. The first aim of this proposal is to investigate the post-prandial muscle protein synthesis rates in young and elderly men in response to a meal-like protein bolus after a period of rest or physical activity (study A). The rest trial (REST) will act as a proof-of-principle study to examine the blunted protein synthetic response in the elderly, and as a control trial in comparison with the exercise trial (EXC) to establish the surplus value of physical activity prior to protein intake on muscle protein synthesis. The second aim of this proposal is to determine the surplus value of an increased quantity of the ingested protein bolus (study B). Large amounts of protein (40 and 60 g) will be compared to a meal-like amount of protein (20 g) as a means to maximize plasma amino acid availability and/or to stimulate muscle protein anabolism. The third aim of this proposal is to study the differences in quality of the ingested protein bolus (study C). Instead of significantly increasing the quantity of the protein bolus, we will also apply a more practical approach to augment skeletal muscle protein synthesis rates; modifying the digestibility or amino acid composition of a meal-like protein bolus.

Conditions

Sarcopenia

Keywords

muscle loss; aging; protein; amino acids; exercise; protein synthesis

Outcome Measures

Primary Outcomes (1)

• All interventions will affect muscle protein synthesis. With the application of amino acid tracer methodology we are able to determine muscle protein synthesis.

  6 hours

Secondary Outcomes (1)

• Differences in rate of uptake/absorption from the intestine.

  6 hours

Study Arms (10)

1

EXPERIMENTAL

Young men 18-30 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids; Other: Physical exercise

2

EXPERIMENTAL

Young men 18-30 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

3

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids; Other: Physical exercise

4

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

5

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

6

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

7

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

8

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

9

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

10

EXPERIMENTAL

Old men 70-85 years, BMI \< 27 kg/m2

Dietary Supplement: Protein/Amino Acids

Interventions

Protein/Amino Acids DIETARY_SUPPLEMENT

Casein Whey Leucine

1; 10; 2; 3; 4; 5; 6; 7; 8; 9

Physical exercise OTHER

The physical exercise protocol will consist of low intensity cycling and light resistance-type exercise; after 5 minutes of self-paced cycling, subjects will perform 6 sets of 10 repetitions on the horizontal leg press machine (Technogym BV, Rotterdam, The Netherlands) and 6 sets of 10 repetitions on the leg extension machine (Technogym BV, Rotterdam, The Netherlands). The first 2 sets of both resistance exercises will be performed at 40% of the subjects' 1RM. Sets 3-4, and 5-6 will be performed at 55% and 75% of 1RM, respectively, with 2 minutes rest intervals between sets.

1; 3

Eligibility Criteria

• Age: 18 Years - 85 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• In study A, non-obese male subjects (BMI \<27) between the age of 18-30 yrs and 70-85 yrs will be selected.
• In studies B and C, non-obese male subjects (BMI \<27) between the age of 70-85 yrs will be selected.

You may not qualify if:

• Type II diabetes or other known diseases
• Use of medication
• Female
• Other ages or BMI than indicated above
• Participation in any regular exercise program.

Sponsors & Collaborators

• Maastricht University Medical Center: lead
• National Nutrition and Food Technology Institute: collaborator

Study Sites (1)

University of Maastricht

Maastricht, Limburg, 6200 MD, Netherlands

Location

Related Publications (4)

• Gorissen SHM, Trommelen J, Kouw IWK, Holwerda AM, Pennings B, Groen BBL, Wall BT, Churchward-Venne TA, Horstman AMH, Koopman R, Burd NA, Fuchs CJ, Dirks ML, Res PT, Senden JMG, Steijns JMJM, de Groot LCPGM, Verdijk LB, van Loon LJC. Protein Type, Protein Dose, and Age Modulate Dietary Protein Digestion and Phenylalanine Absorption Kinetics and Plasma Phenylalanine Availability in Humans. J Nutr. 2020 Aug 1;150(8):2041-2050. doi: 10.1093/jn/nxaa024.

  PMID: 32069356 DERIVED

• Pennings B, Groen B, de Lange A, Gijsen AP, Zorenc AH, Senden JM, van Loon LJ. Amino acid absorption and subsequent muscle protein accretion following graded intakes of whey protein in elderly men. Am J Physiol Endocrinol Metab. 2012 Apr 15;302(8):E992-9. doi: 10.1152/ajpendo.00517.2011. Epub 2012 Feb 14.

  PMID: 22338070 DERIVED

• Pennings B, Boirie Y, Senden JM, Gijsen AP, Kuipers H, van Loon LJ. Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr. 2011 May;93(5):997-1005. doi: 10.3945/ajcn.110.008102. Epub 2011 Mar 2.

  PMID: 21367943 DERIVED

• Pennings B, Koopman R, Beelen M, Senden JM, Saris WH, van Loon LJ. Exercising before protein intake allows for greater use of dietary protein-derived amino acids for de novo muscle protein synthesis in both young and elderly men. Am J Clin Nutr. 2011 Feb;93(2):322-31. doi: 10.3945/ajcn.2010.29649. Epub 2010 Nov 17.

  PMID: 21084649 DERIVED

MeSH Terms

Conditions

Sarcopenia; Muscular Atrophy; Motor Activity

Interventions

Proteins; Amino Acids; Exercise

Condition Hierarchy (Ancestors)

Neuromuscular Manifestations; Neurologic Manifestations; Nervous System Diseases; Atrophy; Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms; Signs and Symptoms; Behavior

Intervention Hierarchy (Ancestors)

Amino Acids, Peptides, and Proteins; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Luc van Loon, Phd

  Maastricht University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 13, 2007
• First Posted: November 14, 2007
• Study Start: December 1, 2007
• Primary Completion: November 1, 2010
• Study Completion: November 1, 2010
• Last Updated: September 14, 2011

Record last verified: 2011-09

Locations

NL (1)