NAC Supplementation and Skeletal Muscle Performance

NCT01778309 | Completed DMC

• 2 other identifiers: NACEXERCISE2011CE-80739
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

In this investigation the investigators utilized NAC administration to foster GSH availability during an 8-day period following eccentric exercise-induced muscle damage in order to test our hypotheses: i) antioxidant supplementation does not disturb performance and adaptations induced by exercise-induced muscle injury and ii) redox status perturbations in skeletal muscle are pivotal for the regulation of muscle' inflammatory response and repair.

Conditions

Skeletal Muscle Damage; Skeletal Muscle Performance; Intgracellular Signaling in Skeletal Muscle; Inflammatory Status

Keywords

aseptic inflammation; skeletal muscle function; exercise

Outcome Measures

Primary Outcomes (24)

• Change in reduced glutathione in blood

  Concentration of reduced glutathione in red blood cells

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in reduced glutathione in muscle

  concentration of reduced glutathione in quadriceps skeletal muscle group

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in protein carbonyls in red blood cells and serum

  concentration of protein carbonyls

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in protein carbonyls in muscle

  protein carbonyl concentration in vastus lateralis skeletal muscle

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in thiobarbituric acid reactive substances in red blood cells and serum

  thiobarbituric acid reactive substances concentration in serum and red blood cells

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in thiobarbituric acid reactive substances in muscle

  thiobarbituric acid reactive substances concentration in vastus lateralis skeletal muscle

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in oxidized glutathione in red blood cells and blood

  Concentration of oxidized glutathione in red blood cells and whole blood

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in total antioxidant capacity in serum

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in oxidized glutathione in muscle

  concentration of oxidized glutathione in vastus lateralis skeletal muscle

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in catalase activity in red blood cells and serum

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in glutathione peroxidase activity in red blood cells

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in creatine kinase activity in plasma

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in C-reactive protein in plasma

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in macrophage infiltration in muscle

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in white blood cell count in blood

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in neutrophil count in blood

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in fatty acid binding protein in plasma

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in cortisol concentration in blood

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in testosterone concentration in plasma

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in cytokine concentration in plasma

  Measurement of IL-1β, IL-4, IL-6, TNF-α, IL-8, IL-10, IL-12p70 concentrations in plasma

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in adhesion molecule concentration in blood

  Measurement of ICAM-1, VCAM-1, sP-selectin, sE-selectin concentrations in plasma

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Change in intracellular signalling proteins in muscle

  Measurement of phosphorylation levels of protein kinase B (Akt), mammalian target of rapamycin (mTOR), serine/threonine kinase (p70S6K), ribosomal protein S6 (rpS6), nuclear factor κB (NFκB), serine⁄threonine mitogen activated protein kinase (p38-MAPK) in vastus lateralis muscle.

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in myogenic determination factor (MyoD) protein levels in muscle

  MyoD expression in vastus lateralis muscle

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

• Change in tumor necrosis factor α in muscle

  Protein levels of TNF-α in vastus lateralis muscle

  one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise

Secondary Outcomes (5)

• Change in muscle function of knee extensor and flexor muscle

  one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise

• Body composition

  One day before exercise

• Maximal aerobic capacity

  One day before exercise

• Change in profile of dietary intake

  one hour before exercise, daily for 8 days post-exercise

• Change in side effect occurence

  one hour before exercise, daily for 8 days post-exercise

Study Arms (1)

n-acetylcysteine/placebo supplementation

EXPERIMENTAL

n-acetylcysteine supplementation, orally in three daily dosages, at 20 mg/kg/day, daily for eight days after exercise placebo, orally in three daily dosages, content: 500 mL drink that contained water (375 mL), sugar-free cordial (125 ml), and 2 g of low-calorie glucose/dextrose powder.

Dietary Supplement: n-acetylcysteine supplementation

Interventions

n-acetylcysteine supplementation DIETARY_SUPPLEMENT

n-acetylcysteine administration: 20 mg//kg/day, orally, daily for eight days following exercise placebo administration: 500 mL orally, daily for eight days following exercise

Also known as: Exercise-induced skeletal muscle damage

n-acetylcysteine/placebo supplementation

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• a) recreationally trained as evidenced by their maximal oxygen consumption levels (VO2max \>45 ml/kg/min), b) were engaged in systematic exercise at least three times/week for ≥12 months), c) non-smokers, d) abstained from any vigorous physical activity during the study, e)abstained from consumption of caffeine, alcohol, performance-enhancing or antioxidant supplements, and medications during the study.

You may not qualify if:

• a) a known NAC intolerance or allergy, b) a recent febrile illness, c) history of muscle lesion, d) lower limb trauma

Sponsors & Collaborators

• Democritus University of Thrace: lead

Study Sites (1)

Laboratory of Physical Education & Sport Performance

Komotini, Thrace, 69100, Greece

Location

Related Publications (1)

• Michailidis Y, Karagounis LG, Terzis G, Jamurtas AZ, Spengos K, Tsoukas D, Chatzinikolaou A, Mandalidis D, Stefanetti RJ, Papassotiriou I, Athanasopoulos S, Hawley JA, Russell AP, Fatouros IG. Thiol-based antioxidant supplementation alters human skeletal muscle signaling and attenuates its inflammatory response and recovery after intense eccentric exercise. Am J Clin Nutr. 2013 Jul;98(1):233-45. doi: 10.3945/ajcn.112.049163. Epub 2013 May 29.

  PMID: 23719546 DERIVED

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Ioannis F Fatouros, Ph.D.

  Democritus University of Thrace, Greece

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: January 21, 2013
• First Posted: January 29, 2013
• Study Start: January 1, 2010
• Primary Completion: September 1, 2011
• Study Completion: April 1, 2012
• Last Updated: January 29, 2013

Record last verified: 2013-01

Locations

GR (1)