Strength Training Induced Alterations in Markers of Immune Function

NCT01450852 | Completed

• 1 other identifier: LSU-IRB # 3005
• Study Type: interventional
• Target: 42
• Locations: 1 country
• Sites: 1

Brief Summary

Exercise has been used to help prevent or slow the progression of inflammation-related disease; however, the mechanism by which this activity may lower concentrations of inflammatory markers remains unclear. The melanocortin receptors 1,3 and 5 (MC1R, MC3R and MC5R) have been shown to function in an anti-inflammatory manner and have the potential to mediate the positive immune adaptations associated with regular physical activity. Preliminary data suggest that MC3R gene expression increases in whole blood after chronic exercise training. The primary aim of the current study is to explore whether this change in gene expression translates into alterations in MC1R, MC3R, or MC5R monocyte surface expression. The secondary aim is to examine the relationship between surface expression of these receptors and circulating inflammatory profiles. The investigators will recruit 42 untrained, healthy males and females aged 18-35 yrs. Half of the group will be placed on an exercise program for 15 weeks. The other half will serve as untrained control subjects. In addition to basic anthropometric measures, the investigators will measure concentrations of inflammatory and anti-inflammatory cytokines (ELISA) and cell surface expression of MC1R, MC3R, and MC5R on monocytes (flow cytometry).

Conditions

Inflammation

Keywords

Melanocortin Receptor; Monocyte; Resistance Training; Inflammation; Markers of Inflammation

Outcome Measures

Primary Outcomes (7)

• Melanocortin 1 Receptor Expression on Monocytes

  Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 1 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

  Before and After 12 Week of Intervention Period

• Plasma CRP

  Blood samples were analyzed for C-reactive protein using ELISA. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

  Before and After the 12 Week Intervention Period

• Body Composition (Fat Tissue Amount)

  Body composition was assessed using DXA. Pre measures were completed in Jan. 2010 and post measures were completed in May 2010 for all subjects.

  Before and After the 12 Week Intervention Period

• Plasma IL-6

  Blood samples were analyzed for IL-6 using ELISA. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

  Before and After the 12 Week Intervention Period

• Plasma IL-10

  Blood samples were analyzed for IL-10 using ELISA. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

  Before and After the 12 Week Intervention Period

• Melanocortin 3 Receptor Cell Surface Expression on Monocytes

  Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 3 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

  Before and After the 12 Week Intervention Period

• Melanocortin 5 Receptor Cell Surface Expression on Monocytes

  Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 5 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

  Before and After the 12 Week Intevention Period

Study Arms (2)

Strength Training

EXPERIMENTAL

The strength training group completed 12 weeks of progressive, periodized resistance training 3d/wk.

Behavioral: Strength Training

Active Control Group

NO INTERVENTION

The active control group was instructed to maintain normal activity and eating habits. They participated in all pre and post intervention measures.

Interventions

Strength Training BEHAVIORAL

Each Member of this group completed 12 weeks of progressive, periodized resistance training for 3d/wk.

Strength Training

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• All Participants Must be Healthy
• The Strength Training Group Members Must Be Approved For Participation by a Licensed MD

You may not qualify if:

• Females may not be pregnant
• Unhealthy participants (those with existing conditions)

Sponsors & Collaborators

• Louisiana State University Health Sciences Center in New Orleans: lead

Study Sites (1)

Louisiana State University

Baton Rouge, Louisiana, 70803, United States

Location

Related Publications (1)

• Henagan TM, Forney L, Dietrich MA, Harrell BR, Stewart LK. Melanocortin receptor expression is associated with reduced CRP in response to resistance training. J Appl Physiol (1985). 2012 Aug;113(3):393-400. doi: 10.1152/japplphysiol.00107.2012. Epub 2012 Jun 7.

  PMID: 22678961 DERIVED

MeSH Terms

Conditions

Inflammation

Interventions

Resistance Training

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Laura K Stewart, PhD

  Louisiana State University Health Sciences Center in New Orleans

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: October 3, 2011
• First Posted: October 12, 2011
• Study Start: January 1, 2010
• Primary Completion: May 1, 2010
• Study Completion: May 1, 2010
• Last Updated: October 12, 2011

Record last verified: 2011-10

Locations

US (1)