Ocular Fluid Work-up in Presumed Trematode Induced Granulomatous Uveitis

NCT07885137 | Recruiting

• 1 other identifier: MD-276-2026
• Study Type: observational
• Target: 40
• Locations: 1 country
• Sites: 1

Brief Summary

Ocular Fluid Work-up in Presumed Trematode Induced Granulomatous Uveitis

Conditions

Presumed Trematode Induced Uveitis; Uveitis

Keywords

PTIGU- granulomatous Uveitis

Outcome Measures

Primary Outcomes (2)

• Testing samples by PCR for DNA Detection

  detect trematodal DNA in the ocular fluid of presumed trematode-induced uveitis by using PCR

  6 month

• Organism detection

  identify the organism responsible for trematode-induced uveitis through DNA sequence analysis of genetic material extracted from ocular fluid.

  6 month

Study Arms (2)

Anterior Uveitis

Anterior Chamber granuloma AC Wash AC fluid Sample

Genetic: AC fluid sample

Intermediate / posterior Uveitis

Ciliary body granuloma PPV and Viteous fluid sample

Genetic: Vitreous fluid sample

Interventions

AC fluid sample GENETIC

PCR using trematoda specific primers DNA sequencing

Anterior Uveitis

Vitreous fluid sample GENETIC

PCR using trematoda specific primer DNA sequencing

Intermediate / posterior Uveitis

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Patients with PTIGU of any age presenting to the Kasr Al-Aini Uveitis and Ocular Immunology Clinic who are scheduled to undergo AC wash or pars plana vitrectomy will be included in the study. The diagnosis of PTIGU will be based on the following criteria:1) history of fresh canal water contact in one of the branches of the River Nile prior to the onset of uveitis, 2) signs of anterior uveitis with the presence of typical AC granuloma 3) signs of intermediate uveitis with the confirmation of CB granuloma by ultrasound biomicroscopy (UBM), 4) absence of clinical signs or work-up suggestive of any other cause of granulomatous Uveitis. 5) Failure of medical treatment by steroids in the form of persistent anterior segment flare and cells \>+1 or posterior segment inflammation (\> Grade 2 vitritis).

You may qualify if:

• Patients with Presumed Trematode-Induced Uveitis scheduled for AC wash or pars plana vitrectomy of any age.

You may not qualify if:

• Causes of granulomatous uveitis other than trematodal infection.
• Inconclusive UBM findings.
• Ocular trauma.
• Previous ocular surgeries including (anterior chamber wash for AC granulomas).

Sponsors & Collaborators

• Kasr El Aini Hospital: lead

Study Sites (1)

Faculty of Medicine Cairo University Kasr Al Ainy Hospital

Cairo, Giza Governorate, 11956, Egypt

RECRUITING

Related Publications (1)

• 1. El Nokrashy, A., Abou Samra, W., Sobeih, D. et al. Treatment of presumed trematode-induced granulomatous anterior uveitis among children in rural areas of Egypt. Eye 33, 1525-1533 (2019). 2. Rathinam SR, Usha KR, Rao NA. Presumed trematode-induced granulomatous anterior uveitis: a newly recognized cause of intraocular inflammation in children from south India. Am J Ophthalmol. 2002 Jun;133(6):773-9. 3. Rathinam S, Fritsche TR, Srinivasan M, Vijayalakshmi P, Read RW, Gautom R, et al. An outbreak of trematode-induced granulomas of the conjunctiva. Ophthalmology. 2001;108(7):1223-9. 4. Amin RM, Goweida MB, El Goweini HF, Bedda AM, Lotfy WM, Gaballah AH, et al. Trematodal granulomatous uveitis in paediatric Egyptian patients: A case series. Br J Ophthalmol. 2017;101(8):999-1002. 5. Amin RH, Abdullatif AM. Management of presumed trematode-induced granulomatous intermediate uveitis. Eye. 2023;37(11):2299-304. 6. Amin RM, Goweida M, Bedda A, Kamel A, Radwan A. Clinical patterns and causes of intraocular inflammation in a uveitis patient cohort from Egypt. Ocul Immunol Inflamm. 2016;26:1-9 7. Arya LK, Rathinam SR, Lalitha P, Kim UR, Ghatani S, Tandon V. Trematode fluke Procerovum varium as cause of ocular inflammation in children, South India. Emerg Infect Dis. 2016;22(2):192-200. 8. Rathinam SR, Arya LK, Usha KR, Prajna L, Tandon V. Novel etiological agent: molecular evidence for trematode-induced anterior uveitis in children. Arch Ophthalmol. 2012;130:1481-4. 9. Amin RM, Radwan AE, Goweida MB, El Goweini HF, Bedda AM, Lotfy WM, et al. Management of presumed trematode induced granulomatous uveitis in pediatric patients. Jpn J Ophthalmol. 2019;63(1):119-25. 10. El Nokrashy A, Abou Samra W, Sobeih D, Lamin A, Hashish A, Tarshouby S, et al. Treatment of presumed trematode-induced granulomatous anterior uveitis among children in rural areas of Egypt. Eye. 2019;33(10):1525-33.

  BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Aqueous fluid Vitreous Fluid

MeSH Terms

Conditions

Uveitis

Condition Hierarchy (Ancestors)

Uveal Diseases; Eye Diseases

Central Study Contacts

Amnah A Amin, Master degree ophthalmology

CONTACT

01006371579; amnah95@hotmail.com

Abdussalam M Abdullatif, Assistant Professor

CONTACT

01117039868; abdussalamabdullatif@kasralainy.edu.eg

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Target Duration: 6 Months
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer of Ophthalmology Cairo University

Study Record Dates

• First Submitted: October 7, 2026
• First Posted: October 21, 2026
• Study Start: September 1, 2026
• Primary Completion (Estimated): September 1, 2027
• Study Completion (Estimated): December 1, 2027
• Last Updated: October 21, 2026

Record last verified: 2026-10

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)