Acute Effects of HIIT and MICT on Catestatin Dynamics in Young Males

NCT07819422 | Completed

• 2 other identifiers: 2025/09-13125S394
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Physical exercise acts as a major physiological stressor that alters cardiovascular and autonomic homeostasis. Catestatin (CTS), a bioactive peptide derived from chromogranin A (CgA), acts as a potent inhibitor of catecholamine secretion and modulates autonomic cardiovascular tone. However, the acute effects of distinct exercise modalities on circulating CTS dynamics and their associations with post-exercise autonomic recovery remain unclarified. The purpose of this study is to investigate the acute responses of serum catestatin and chromogranin A to high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) in healthy sedentary young males, and to evaluate their potential relationship with post-exercise heart rate recovery (HRR). Participants completed an initial incremental performance test followed by either a single HIIT session or a MICT session on a cycle ergometer. Serial blood samples and continuous cardiovascular measurements were obtained before, immediately after, and throughout a 60-minute post-exercise recovery period.

Conditions

Healthy Volunteers; Exercise Physiology; Autonomic Nervous System Regulation

Keywords

Catestatin; Chromogranin A; High-intensity interval training; Moderate-intensity continuous training; Heart rate recovery; Parasympathetic reactivation

Outcome Measures

Primary Outcomes (1)

• Serum Catestatin Concentration

  Serum catestatin levels measured using enzyme-linked immunosorbent assay (ELISA) to evaluate acute exercise-induced dynamic responses.

  Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.

Secondary Outcomes (2)

• Serum Chromogranin-A Concentration

  Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.

• Post-Exercise Heart Rate Recovery (HRR1 and HRR5)

  At 1 minute and 5 minutes post-exercise.

Study Arms (2)

High-Intensity Interval Training (HIIT)

EXPERIMENTAL

Participants completed an acute HIIT session on a cycle ergometer consisting of a 5-minute warm-up, followed by four 4-minute bouts at 85-95% of maximum heart rate (HRmax) interleaved with 3-minute active recovery periods, followed by a 5-minute cool-down.

Other: High-Intensity Interval Exercise

Moderate-Intensity Continuous Training (MICT)

EXPERIMENTAL

Participants completed an acute MICT session on a cycle ergometer consisting of a 5-minute warm-up, followed by 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), followed by a 5-minute cool-down.

Other: Moderate-Intensity Continuous Exercise

Interventions

High-Intensity Interval Exercise OTHER

Acute bout of 4x4 min intervals at 85-95% HRmax with 3 min active recovery on a cycle ergometer.

High-Intensity Interval Training (HIIT)

Moderate-Intensity Continuous Exercise OTHER

Acute bout of continuous cycling for 30 minutes at 60-70% HRmax on a cycle ergometer.

Moderate-Intensity Continuous Training (MICT)

Eligibility Criteria

• Age: 18 Years - 25 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy male individuals aged 18-25 years
• Body mass index (BMI) between 18.0 and 25.0 kg/m²
• Sedentary lifestyle (no regular exercise engagement within the last 3 months; \<90 min/week moderate-intensity or \<60 min/week vigorous-intensity exercise)
• Normal resting blood pressure (\<140/90 mmHg)
• Fasting plasma glucose between 60 and 100 mg/dL
• Normal resting electrocardiogram (ECG) and pulmonary function tests (FEV1/FVC \>80%)
• Signed written informed consent

You may not qualify if:

• Regular smoking or alcohol consumption
• Any current or regular medication use
• History or clinical signs of cardiovascular, respiratory, metabolic, or renal diseases
• Symptoms suggestive of cardiopulmonary/metabolic disease (chest pain, shortness of breath, dizziness, syncope, ankle edema, palpitations, intermittent claudication)
• Presence of known cardiovascular risk factors
• Iron deficiency / anemia
• Musculoskeletal limitations or contraindications to maximal cycle ergometer testing

Sponsors & Collaborators

• Firat University: lead
• The Scientific and Technological Research Council of Turkey: collaborator

Study Sites (1)

Firat University Medical Faculty, Department of Physiology

Elâzığ, Elâzığ, 23119, Turkey (Türkiye)

Location

MeSH Terms

Interventions

High-Intensity Interval Training

Intervention Hierarchy (Ancestors)

Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: MD

Model Details: A two-arm parallel-group design comparing acute high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) in sedentary young males.

Study Record Dates

• First Submitted: September 9, 2026
• First Posted: September 15, 2026
• Study Start: October 24, 2025
• Primary Completion: January 10, 2026
• Study Completion: March 5, 2026
• Last Updated: September 15, 2026

Record last verified: 2025-07

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP
• Time Frame: Beginning immediately following publication with no end date.
• Access Criteria: Data will be shared for non-commercial academic research purposes upon written request to the corresponding author.

Locations

TU (1)