Exercise-Induced Changes in Serum Oncostatin M Levels in Recreationally Active Males

NCT07195630 | Completed

• 1 other identifier: 2023/02-27
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study was to compare the effects of workload-matched acute moderate-intensity continuous exercise (MICE) and high-intensity interval exercise (HIIE) on serum OSM levels on a cycle ergometer in recreationally active, healthy men. Fifteen participants participated in both MICE and HIIE sessions. The HIIE session consisted of 10 x 1 min intervals at 90% of maximal oxygen uptake (VO2max) separated by a 75-second active recovery period at 60 W, whereas the MICE session consisted of a \~21 min exercise session at 55% of VO2max. Blood samples were collected before (Pre) and immediately after (Post) acute MICE and HIIE sessions. Serum OSM levels were measured by the ELISA method.

Conditions

The Aim of This Study is to Evaluate the Effects of Acute Exercise on Serum Oncostatin M Levels in Healthy, Recreationally Active Males

Keywords

Acute Exercise; HIIT; Oncostatin M; MICE

Outcome Measures

Primary Outcomes (1)

• Change from Pre- to Post-Exercise Levels of Oncostatin M

  Serum samples were collected at pre- and post-exercise time points, and serum oncostatin M levels were measured using a commercial ELISA kit

  From baseline assessment of blood samples at pre-exercise to the assessment of blood sample analysis at the end of the post-exercise.

Study Arms (2)

Moderate-intensity continuous exercise (MICE)

EXPERIMENTAL

15 recreationally active males completed the \~21 min of exercise at 55% of VO2max, consisting of a 3-minute warm-up and 3-minute cool-down at 60 W.

Other: Moderate-Intensity Continuous Exercise

High-intensity interval exercise (HIIE)

EXPERIMENTAL

15 recreationally active males performed 10 x 1-minute intervals at 90% of VO2max, separated by 75 s of active recovery at 60 W, and a final 3-minute cool-down period at 60 W.

Other: High-Intensity Interval Exercise

Interventions

Moderate-Intensity Continuous Exercise OTHER

15 recreationally active males completed the \~21 min of exercise at 55% of VO2max, consisting of a 3-minute warm-up and 3-minute cool-down at 60 W.

Moderate-intensity continuous exercise (MICE)

High-Intensity Interval Exercise OTHER

15 recreationally active males performed 10 x 1-minute intervals at 90% of VO2max, separated by 75 s of active recovery at 60 W, and a final 3-minute cool-down period at 60 W.

High-intensity interval exercise (HIIE)

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Engaged in regular exercise/training in the last 6 months,
• years of age,
• Body mass index (BMI) in the range of 18.5-24.9 kg/m².

You may not qualify if:

• Having a chronic disease that limits exercise,
• Smoking,
• Using any medication or supplement that may affect metabolism.

Sponsors & Collaborators

• Hacettepe University: lead

Study Sites (1)

Hacettepe University

Ankara, Çankaya, Turkey (Türkiye)

Location

MeSH Terms

Interventions

High-Intensity Interval Training

Intervention Hierarchy (Ancestors)

Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: OTHER
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: LECTURER

Study Record Dates

• First Submitted: September 13, 2025
• First Posted: September 26, 2025
• Study Start: August 1, 2023
• Primary Completion: August 1, 2024
• Study Completion: September 20, 2024
• Last Updated: September 26, 2025

Record last verified: 2025-09

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)