GFI-1 and Periodontal Disease

NCT07758374 | Completed

• 1 other identifier: IU-CAP-TCD-2022-2717
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Gum disease, including gingivitis and its more advanced form, periodontitis, is a common condition that damages the tissues supporting the teeth. This study examines how gum disease affects the expression of specific genes and proteins that regulate communication between internal cell structures and the clearance of damaged material. Researchers will collect small gum tissue samples from people with healthy gums, gingivitis, or periodontitis during dental procedures they are already undergoing. These samples will be used to measure the expression levels of the GFI1, NF-κB, and NLRP3 genes/proteins, along with IL-1beta levels. In a separate laboratory experiment, gum cells will be exposed to a substance produced by bacteria commonly associated with gum disease, to observe how these same genes and protein respond. The goal of this study is to better understand the biological changes that occur in gum tissue as gum disease progresses, which may help guide future research into new treatments.

Conditions

Periodontitis; Gingivitis

Keywords

periodontitis; gingivitis; inflammation; inflammasome

Outcome Measures

Primary Outcomes (1)

• GFI1, NLRP3, NF-KB, IL-1beta Gene and Protein Expression

  Gene expression (RT-PCR, relative fold change via 2\^-ΔΔCT method) and protein expression (Western blot, band intensity normalized to ACTB) of GFI1, NLRP3, NF-KB, IL-1beta were measured and compared in gingival tissue biopsies from participants with periodontal health, gingivitis, and periodontitis

  January to July 2024

Study Arms (3)

periodontally healthy

periodontally healthy participants

gingivitis

patients with gingivitis

periodontitis

patients with periodontitis

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Adults aged 18 to 60 years attending the periodontology clinic were enrolled and classified into three groups based on clinical periodontal parameters according to the 2017 World Workshop classification: periodontal health, gingivitis, and periodontitis (Stage III). Exclusion criteria included any systemic disease, regular medication use, smoking, pregnancy or lactation, periodontal treatment within the past 6 months, antibiotic use within the past 6 months, and presence of prosthetic restorations on the teeth to be sampled. A total of 60 participants (20 per group; 30 male, 30 female) were included.

Sponsors & Collaborators

• Inonu University: lead

Study Sites (1)

Inonu University

Malatya, 44100, Turkey (Türkiye)

Location

Related Publications (4)

• Zhu L, Meng Q, Liang S, Ma Y, Li R, Li G, Zeng H. The transcription factor GFI1 negatively regulates NLRP3 inflammasome activation in macrophages. FEBS Lett. 2014 Nov 28;588(23):4513-9. doi: 10.1016/j.febslet.2014.10.025.

  PMID: 25447538 BACKGROUND

• Hock H, Orkin SH. Zinc-finger transcription factor Gfi-1: versatile regulator of lymphocytes, neutrophils and hematopoietic stem cells. Curr Opin Hematol. 2006 Jan;13(1):1-6. doi: 10.1097/01.moh.0000190111.85284.8f.

  PMID: 16319680 BACKGROUND

• Aral K, Berdeli E, Cooper PR, Milward MR, Kapila Y, Karadede Unal B, Aral CA, Berdeli A. Differential expression of inflammasome regulatory transcripts in periodontal disease. J Periodontol. 2020 May;91(5):606-616. doi: 10.1002/JPER.19-0222. Epub 2019 Oct 17.

  PMID: 31557327 BACKGROUND

• Aral K, Milward MR, Kapila Y, Berdeli A, Cooper PR. Inflammasomes and their regulation in periodontal disease: A review. J Periodontal Res. 2020 Aug;55(4):473-487. doi: 10.1111/jre.12733. Epub 2020 Jan 20.

  PMID: 31960443 BACKGROUND

MeSH Terms

Conditions

Periodontitis; Gingivitis; Inflammation

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Infections; Gingival Diseases; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: August 6, 2026
• First Posted: August 11, 2026
• Study Start: January 1, 2024
• Primary Completion: July 15, 2024
• Study Completion: July 15, 2024
• Last Updated: August 11, 2026

Record last verified: 2026-08

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP
• Time Frame: Beginning 6 months and ending 24 months following article publication
• Access Criteria: Data will be made available to researchers who provide a methodologically sound proposal, subject to approval by the corresponding author, for the purpose of achieving the aims outlined in the approved proposal.

Locations

TU (1)