PTPIP51-VAPB Complex in the Periodontal Disease

NCT07705048 | Completed

• 1 other identifier: IU-2024/13
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Gum disease, including gingivitis and more advanced periodontitis, is a common condition that damages the tissues supporting the teeth. This study looks at how gum disease affects specific proteins inside gum tissue cells that help control how cells communicate between their internal structures and clean out damaged material (a process called autophagy). Researchers will collect small gum tissue samples from people with healthy gums, gingivitis, or periodontitis during dental procedures they are already having. These samples will be tested to measure the levels of three proteins (called PTPIP51, VAPB, and LC3B) and calcium. In a separate laboratory experiment, gum cells will be exposed to a substance made by bacteria commonly linked to gum disease, to see how these same proteins respond. The goal of this study is to better understand the biological changes that happen in gum tissue as gum disease develops, which may help guide future research into new treatments.

Conditions

Periodontitis; Gingivitis

Keywords

periodontitis; gingivitis; mitochondria; endoplasmic reticulum

Outcome Measures

Primary Outcomes (1)

• Gingival PTPIP51, VAPB, and LC3B Gene and Protein Expression

  Gene expression (RT-PCR, relative fold change via 2\^-ΔΔCT method) and protein expression (Western blot, band intensity normalized to ACTB) of PTPIP51, VAPB, and LC3B were measured and compared in gingival tissue biopsies from participants with periodontal health, gingivitis, and periodontitis

  From February to July 2024

Study Arms (3)

periodontally healthy

periodontally healthy participants

gingivitis group

patients with gingivitis

periodontitis

patients with periodontitis

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Adults aged 18 to 60 years attending the periodontology clinic were enrolled and classified into three groups based on clinical periodontal parameters according to the 2017 World Workshop classification: periodontal health, gingivitis, and periodontitis (Stage III). Exclusion criteria included any systemic disease, regular medication use, smoking, pregnancy or lactation, periodontal treatment within the past 6 months, antibiotic use within the past 6 months, and presence of prosthetic restorations on the teeth to be sampled. A total of 60 participants (20 per group; 30 male, 30 female) were included.

Sponsors & Collaborators

• Inonu University: lead

Study Sites (1)

Inonu University

Malatya, 44100, Turkey (Türkiye)

Location

Related Publications (3)

• Kirmizigul OA, Sabanci A, Disli F, Yildiz S, Milward MR, Aral K. Evaluation of the role of mitofusin-1 and mitofusin-2 in periodontal disease. J Periodontol. 2024 Jan;95(1):64-73. doi: 10.1002/JPER.23-0072. Epub 2023 Jul 24.

  PMID: 37436713 BACKGROUND

• Aral K, Milward MR, Cooper PR. Gene expression profiles of mitochondria-endoplasmic reticulum tethering in human gingival fibroblasts in response to periodontal pathogens. Arch Oral Biol. 2021 Aug;128:105173. doi: 10.1016/j.archoralbio.2021.105173. Epub 2021 May 27.

  PMID: 34058723 BACKGROUND

• Gomez-Suaga P, Paillusson S, Stoica R, Noble W, Hanger DP, Miller CCJ. The ER-Mitochondria Tethering Complex VAPB-PTPIP51 Regulates Autophagy. Curr Biol. 2017 Feb 6;27(3):371-385. doi: 10.1016/j.cub.2016.12.038. Epub 2017 Jan 26.

  PMID: 28132811 BACKGROUND

MeSH Terms

Conditions

Periodontitis; Gingivitis

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Infections; Gingival Diseases

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Target Duration: 1 Day
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: July 10, 2026
• First Posted: July 15, 2026
• Study Start: February 1, 2024
• Primary Completion: July 30, 2024
• Study Completion: July 30, 2024
• Last Updated: July 15, 2026

Record last verified: 2026-07

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP
• Time Frame: Beginning 6 months and ending 24 months following article publication
• Access Criteria: Data will be made available to researchers who provide a methodologically sound proposal, subject to approval by the corresponding author, for the purpose of achieving the aims outlined in the approved proposal.

Locations

TU (1)