Hypochlorous Acid in Non-Surgical Periodontal Therapy

NCT07712627 | Completed

• 2 other identifiers: ATAUNI-PERIO-HOCL-2025-001BAP-16024
• Study Type: interventional
• Target: 44
• Locations: 1 country
• Sites: 1

Brief Summary

Periodontitis is a chronic inflammatory disease associated with dental plaque and characterized by the progressive destruction of the tooth-supporting tissues. The principal features of periodontitis include clinical attachment loss (CAL), alveolar bone loss, periodontal pocket formation, gingival bleeding, and the subsequent loss of periodontal tissue support. The current gold standard for the management of periodontal disease comprises oral hygiene instruction (OHI), non-surgical periodontal therapy (NSPT), periodontal surgery when indicated, and supportive periodontal therapy (SPT). In addition, various antimicrobial agents are used as adjuncts to periodontal treatment. One such agent, hypochlorous acid (HOCl), is a potent antimicrobial with high affinity against Gram-negative bacteria within the subgingival biofilm. Hypochlorous acid is commercially available in mouthwash and spray formulations, which patients may use as adjunctive therapies following periodontal treatment. In the present project, hypochlorous acid will be applied by the clinician using a dental device during periodontal treatment in a clinical setting. Changes in both clinical and biochemical outcomes, including high-sensitivity C-reactive protein (hs-CRP), C-reactive protein (CRP), antistreptolysin O (ASO), and oncostatin M (OSM), will be recorded and compared with those obtained following conventional periodontal treatment performed using purified water. The novelty of the proposed study lies in the first-time use of hypochlorous acid as an irrigant in place of purified water during scaling and root planing (SRP), with the aim of evaluating its effects on both clinical and biochemical parameters. This project is expected to attract the attention of researchers investigating the treatment of periodontitis and may facilitate publication in high-impact international peer-reviewed journals as well as presentation at prestigious scientific congresses. Furthermore, it will contribute to the training of qualified researchers in periodontology and support the completion of a doctoral dissertation. The findings may also provide preliminary data for future studies aimed at developing hypochlorous acid-based adjunctive therapeutic approaches for the management of periodontitis.

Conditions

Periodontitis; Periodontitis Stage I; Periodontitis Stage II

Keywords

PERİODONTİTİS; CRP; hs-CRP; ONCOSTATİN M; Anti-streptolysin O; Hypochlorous acid; Non-surgical Periodontal Therapy

Outcome Measures

Primary Outcomes (4)

• Clinical Attachment Level (CAL)

  Assessment of clinical attachment level (CAL), measured in millimeters, as an indicator of periodontal tissue healing.

  Baseline, 1 month and 3 months

• Probing Pocket Depth (PPD)

  Assessment of probing pocket depth (PPD), measured in millimeters, to evaluate clinical periodontal healing.

  Baseline, 1 month and 3 months

• Plaque Index (PI)

  Assessment of dental plaque accumulation.

  Baseline, 1 month and 3 months

• Bleeding on Probing (BOP)

  Assessment of gingival bleeding following periodontal probing.

  Baseline, 1 month and 3 months

Secondary Outcomes (4)

• C-Reactive Protein (CRP)

  Baseline, 1 month, and 3 months

• High-Sensitivity C-Reactive Protein (hs-CRP)

  Baseline, 1 month and 3 months

• Oncostatin M (OSM)

  Baseline, 1 month and 3 months

• Antistreptolysin O (ASO)

  Baseline, 1 month and 3 months

Study Arms (2)

Scaling and Root Planing (SRP) with Hypochlorous Acid

EXPERIMENTAL

Participants will undergo scaling and root planing (SRP) using hypochlorous acid.

Procedure: Hypochlorous Acid Application

Scaling and Root Planing (SRP) with Purified Water

ACTIVE COMPARATOR

Participants will undergo scaling and root planing (SRP) using an ultrasonic device with purified water.

Procedure: Scaling and Root Planing (SRP) with Purified Water

Interventions

Hypochlorous Acid Application PROCEDURE

Scaling and root planing (SRP) will be performed using an ultrasonic device with a hypochlorous acid solution.

Scaling and Root Planing (SRP) with Hypochlorous Acid

Scaling and Root Planing (SRP) with Purified Water PROCEDURE

Scaling and root planing (SRP) will be performed using an ultrasonic device with purified water.

Scaling and Root Planing (SRP) with Purified Water

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Individuals aged 18 to 65 years.
• Systemically healthy individuals.
• Diagnosis of Stage I or Stage II periodontitis according to the 2017 Classification of Periodontal Diseases.
• Probing pocket depth (PPD) ≤5 mm and clinical attachment loss (CAL) ≤4 mm.
• Presence of a sufficient number of teeth for periodontal evaluation.
• Willingness to participate and ability to provide written informed consent.

You may not qualify if:

• Patients with contraindications to the use of ultrasonic instrumentation.
• Pregnant or lactating women.
• Patients receiving anti-inflammatory or antibiotic therapy.
• Patients with systemic diseases or medical conditions.
• Patients receiving continuous medication for any medical condition.
• Patients who have undergone periodontal treatment within the previous 6 months.
• Patients presenting with acute dental pain (e.g., pulpitis or apical/periodontal abscess).
• Individuals who smoke or consume alcohol.
• Presence of fewer than 15 teeth.

Sponsors & Collaborators

• Ataturk University: lead

Study Sites (1)

Faculty of Dentistry, Atatürk University

Erzurum, Erzurum, Turkey (Türkiye)

Location

Related Links

• Provides general information about the hypochlorous acid used in this study.
• Provides general information about periodontitis.

MeSH Terms

Conditions

Periodontitis; Dry Socket

Interventions

Tooth Exfoliation; Root Planing

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Intervention Hierarchy (Ancestors)

Dental Physiological Phenomena; Digestive System and Oral Physiological Phenomena; Dental Scaling; Dental Prophylaxis; Periodontics; Dentistry; Subgingival Curettage; Preventive Dentistry

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Masking Details: The clinical outcome assessor will be blinded to group allocation. The clinician performing the intervention will be aware of the assigned treatment; however, participants will not be explicitly informed of their group allocation.
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: Participants will be randomly allocated into two parallel groups: a test group receiving scaling and root planing (SRP) using hypochlorous acid and a control group receiving scaling and root planing (SRP) using purified water.

Study Record Dates

• First Submitted: June 14, 2026
• First Posted: July 17, 2026
• Study Start: October 9, 2025
• Primary Completion: May 15, 2026
• Study Completion: June 12, 2026
• Last Updated: July 17, 2026

Record last verified: 2026-07

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)