Effects of PRF and i-PRF on Clinical and Biochemical Parameters in Non-Surgical Periodontal Therapy

NCT07525531 | Completed

• 2 other identifiers: OMU-KAEK- 2023/344BAP04-B-2024-5249
• Study Type: interventional
• Target: 56
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this clinical trial is to learn if adding platelet-rich fibrin (PRF) or injectable platelet-rich fibrin (i-PRF), obtained from the participant's own blood, to standard non-surgical periodontal treatment helps improve gum health in individuals with periodontitis. It will also assess how these applications affect inflammation and bone-related markers in the gum fluid. The main questions it aims to answer are :

• Does the application of PRF or i-PRF improve clinical periodontal healing compared to standard treatment alone?
• Do these applications influence the levels of inflammatory and bone metabolism-related biomarkers in gingival crevicular fluid? Researchers will compare patients receiving PRF or i-PRF in addition to standard periodontal treatment with those receiving standard treatment alone. In addition, the findings obtained from individuals with periodontal disease will be compared with data from periodontally healthy individuals. Participants will:
• Receive standard periodontal treatment (scaling and root planing)
• Receive PRF or i-PRF depending on their assigned group
• Visit the clinic before treatment and at 2, 4, and 6 weeks after treatment for checkups

Conditions

Periodontitis

Keywords

Periodontitis; Platelet Rich Fibrin; Non-Surgical Periodontal Therapy; Gingival Crevicular Fluid; IL-1ß; TNF-α; IL-6; OPG; RANKL

Outcome Measures

Primary Outcomes (1)

• Change in probing depth (PD)

  Change in probing depth (PD) measured in millimeters at six sites per tooth using a periodontal probe, comparing baseline values with follow-up measurements.

  Baseline, 2 weeks, 4 weeks, and 6 weeks

Secondary Outcomes (9)

• Change in clinical attachment level (CAL)

  Baseline, 2 weeks, 4 weeks, and 6 weeks

• Change in plaque index (PI)

  Baseline, 2 weeks, 4 weeks, and 6 weeks

• Change in gingival index (GI)

  Baseline, 2 weeks, 4 weeks, and 6 weeks

• Change in bleeding on probing (BoP)

  Baseline, 2 weeks, 4 weeks, and 6 weeks

• Change in gingival crevicular fluid (GCF) IL-1β levels

  Baseline, 2 weeks, and 6 weeks

Study Arms (4)

Non-surgical periodontal therapy (NSPT)

ACTIVE COMPARATOR

Participants received non-surgical periodontal therapy (NSPT), including full-mouth scaling and root planing (SRP). No adjunctive biomaterial was applied.

Procedure: Non-surgical periodontal therapy (NSPT)

NSPT + PRF

EXPERIMENTAL

Participants received non-surgical periodontal therapy (NSPT), including full-mouth scaling and root planing (SRP). Following NSPT, platelet-rich fibrin (PRF) was applied into periodontal pockets.

Procedure: Non-surgical periodontal therapy (NSPT); Biological: Platelet-rich fibrin (PRF)

NSPT + i-PRF

EXPERIMENTAL

Participants received non-surgical periodontal therapy (NSPT), including full-mouth scaling and root planing (SRP). Following NSPT, injectable platelet-rich fibrin (i-PRF) was applied into periodontal pockets.

Procedure: Non-surgical periodontal therapy (NSPT); Biological: Injectable platelet-rich fibrin (i-PRF)

Healthy control

NO INTERVENTION

Periodontally and systemically healthy participants were included as controls. No periodontal treatment was performed. Gingival crevicular fluid (GCF) samples were collected, and oral hygiene instructions were provided

Interventions

Non-surgical periodontal therapy (NSPT) PROCEDURE

Non-surgical periodontal therapy (NSPT) consisted of full-mouth scaling and root planing (SRP), including the removal of supra- and subgingival deposits using ultrasonic devices and hand instruments.

NSPT + PRF; NSPT + i-PRF; Non-surgical periodontal therapy (NSPT)

Platelet-rich fibrin (PRF) BIOLOGICAL

Platelet-rich fibrin (PRF) was prepared from autologous venous blood by centrifugation at 2700 rpm for 12 minutes, processed into a membrane, adapted to the size of the periodontal pocket, and placed into the periodontal pocket using hand instruments.

NSPT + PRF

Injectable platelet-rich fibrin (i-PRF) BIOLOGICAL

Injectable platelet-rich fibrin (i-PRF) was prepared from autologous venous blood by low-speed centrifugation at 700 rpm for 3 minutes and injected into periodontal pockets.

NSPT + i-PRF

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Voluntary consent to participate in the study
• Individuals aged 18 years or older
• Systemically healthy individuals
• Abstaining from the use of any drugs for any purpose
• No periodontal treatment history in the past six months
• Individuals who do not smoke

You may not qualify if:

• Absence of willingness to engage in the studies
• Individuals under the age of 18
• Existence of any systemic disease influencing periodontal condition and consistent medication usage
• Administration of local or systemic antibiotics within the preceding six months
• Chronicle of periodontal therapy during the past six months
• History of any periodontal surgical intervention within the past 12 months
• Pregnancy or lactation in female subjects
• Teeth with endodontic lesion or mobility degrees 2 and 3

Sponsors & Collaborators

• Ondokuz Mayıs University: lead

Study Sites (1)

Ondokuz Mayıs University

Samsun, Atakum, 55200, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Periodontitis

Interventions

proliferation regulatory factors, human urine

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: principle investigator, periodontist

Study Record Dates

• First Submitted: April 6, 2026
• First Posted: April 13, 2026
• Study Start: April 20, 2024
• Primary Completion: August 5, 2025
• Study Completion: December 25, 2025
• Last Updated: April 15, 2026

Record last verified: 2026-04

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)