NCT07630246

Brief Summary

Lung cancer remains a significant challenge in oncology, with poor prognosis for patients, especially those with advanced-stage disease. The phenomenon of tumor spread through air spaces (STAS) in pulmonary cancer has garnered attention for its association with aggressive tumor behavior and adverse clinical outcomes. Spread through air spaces identification has been highly debatable on scientific community as an important prognostic feature for distant and locoregional recurrence and as a key player in the differential diagnosis and selection of the appropriate treatment. The aim of this study is to unravel the complexities of STAS-positive lung adenocarcinoma (LUAD) diagnosis and treatment options. For that, we intend to (1) isolate primary lung cancer tissues from early-stages lung adenocarcinoma to fabricate organoid in vitro models and (2) evaluate the microRNA (miRNA) profile of tumor and healthy tissue samples. This is a Hybrid (prospective and retrospective) observational clinical study with a nested translational study.

Trial Health

63
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Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
140

participants targeted

Target at P50-P75 for all trials

Timeline
38mo left

Started Jun 2026

Typical duration for all trials

Geographic Reach
1 country

2 active sites

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Progress1%
Jun 2026Jun 2029

First Submitted

Initial submission to the registry

June 1, 2026

Completed
Same day until next milestone

Study Start

First participant enrolled

June 1, 2026

Completed
4 days until next milestone

First Posted

Study publicly available on registry

June 5, 2026

Completed
2.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 30, 2028

Expected
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

June 30, 2029

Last Updated

June 5, 2026

Status Verified

June 1, 2026

Enrollment Period

2.1 years

First QC Date

June 1, 2026

Last Update Submit

June 1, 2026

Conditions

Keywords

Pulmonary cancer, Lung, Tumor spread through air spaces

Outcome Measures

Primary Outcomes (1)

  • Identification and quantification of specific gene miRNAs overexpression's

    \- Identify and quantify specific gene miRNAs ubiquitously overexpressed in paraffined tumor tissue and in blood samples of STAS-positive patients in comparison with other LUAD patient group.

    1 year

Secondary Outcomes (1)

  • Creation of in vitro model to recapitulate STAS-positive LUAD microenvironment

    1 year

Study Arms (2)

Retrospective Cohort I: 70 patients (35 patients for group 1 and 35 patients for group 2)

Group 1: patients newly diagnosed with early-stage LUAD, STAS negative Group 2: patients newly diagnosed with early-stage LUAD, STAS positive

Prospective Cohort II: 70 patients (35 patients for group 1 and 35 patients for group 2)

Group 1: patients newly diagnosed with early-stage LUAD, STAS negative Group 2: patients newly diagnosed with early-stage LUAD, STAS positive

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Cohort I: Retrospective data collection 70 patients who have undergone LUAD surgery at the Thoracic Surgery Unity of ULS DE SÃO JOSÉ (35 patients for group 1 and 35 patients for group 2). In this cohort, we use banked formalin-fixed paraffin embedded (FFPE) tissue samples for analysis. Cohort II: Prospective data Collection 70 patients diagnosed with LUAD in ULSSJ will be invited to participate in the study (35 patients for group 1 and 35 patients for group 2). In this cohort of patients, we will prospectively perform a non-interventional study to recruit LUAD patients and analyze the clinical impact of the STAS pattern in patients with LUAD.

You may qualify if:

  • Patients older than 18 years old
  • Active early LUAD diagnosis according to the American Joint Committee on Cancer
  • Signed informed consent or previous consent given for future research

You may not qualify if:

  • History of inflammatory bowel disease and autoimmune diseases
  • History of hepatic disease (including history of alcoholic or viral hepatitis)
  • LUAD patients with other active malignancy(ies)
  • Previous treatment for LUAD
  • Incapability of understanding the study and/or providing consent
  • Signed informed consent unavailable

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

NMS Research | Cancer Nanomedicine - João Conde's Lab

Lisbon, Portugal

Location

Unidade Local de Saúde de São José

Lisbon, Portugal

Location

Biospecimen

Retention: SAMPLES WITH DNA

Paraffined tumor tissue and blood samples

MeSH Terms

Conditions

Adenocarcinoma of LungLung Neoplasms

Condition Hierarchy (Ancestors)

AdenocarcinomaCarcinomaNeoplasms, Glandular and EpithelialNeoplasms by Histologic TypeNeoplasmsRespiratory Tract NeoplasmsThoracic NeoplasmsNeoplasms by SiteLung DiseasesRespiratory Tract Diseases

Central Study Contacts

Bárbara da Silva Mendes, PhD

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
OTHER
Target Duration
1 Year
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 1, 2026

First Posted

June 5, 2026

Study Start

June 1, 2026

Primary Completion (Estimated)

June 30, 2028

Study Completion (Estimated)

June 30, 2029

Last Updated

June 5, 2026

Record last verified: 2026-06

Data Sharing

IPD Sharing
Will not share

IPD will not be shared due to data protection, privacy, and confidentiality considerations. The dataset may contain sensitive health information and the informed consent obtained from participants does not include provisions for broad data sharing with external researchers. Aggregate results will be published in peer-reviewed journals and may be made publicly available, but IPD access will be limited to authorized study personnel for the purposes of analysis and regulatory compliance.

Locations