Effects of BFR-training on Stem Cells and Immune Cells in Human Skeletal Muscle

NCT07569627 | Completed

• 2 other identifiers: IRB-2021-082MOST 111-2410-H-845 -005 -MY3
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

In this 3-year project, investigators will first measure stem cells (primary outcome) and immune cells (secondary outcome) in human skeletal muscle immediately after and 24 hour after the BFR-training. In the second and third year, the effect of β-NMN (vitamin B3 precursor) and antioxidant on the same outcomes after BFR-exercise will be assessed. The BFR-exercise in the study will consist of a 5 × 10-second sprinting with maximal voluntary efforts, 5 minutes after a BFR pre-conditioning to legs using pressurized cuffs. In Study 1 (1st year, N = 24, aged 20-30 year), a randomized crossover trial will be conducted to compare the efficacy of sprinting exercise using BFR pre-conditioning at 20 mmHg (control) or 180 mmHg (occlusion). Study 2 (2nd year, N = 24, aged 20-30 years) will be conducted to examine the effect of β-NMN supplementation on the aforementioned outcomes in human muscles using a randomized placebo-controlled crossover study design. Study 3 (third year, N = 24, aged 20-30 years) will be conducted to examine the effect of vitamin C on the same variables immediately after and 24 hours after resistance exercise intervention, using a randomized placebo-controlled crossover study design.

Conditions

Exercise; Blood Flow Restriction (BFR) Training Effects; Supplement

Keywords

NMN; vitamin C; exercise; BFR

Outcome Measures

Primary Outcomes (1)

• Stem cells in muscle

  Stem cells are identified by Stro-1 cell marker

  Pre-exercise, immediately post-exercise, and 24-hour post-challenge (BFR-exercise) recovery

Secondary Outcomes (1)

• Immune cells in muscle

  At 24-hour post-challenge recovery

Study Arms (6)

Sham (20 mmHg)

SHAM COMPARATOR

Study 1: Sham: blood flow restriction (20 mmHg on leg) for 3-5 min before exercise

Behavioral: BFR (180 mmHg)

BFR (180 mmHg)

EXPERIMENTAL

Study 1: BFR: blood flow restriction (180 mmHg on leg) for 3-5 min before exercise

Behavioral: BFR (180 mmHg)

Placebo-BFR exercise

PLACEBO COMPARATOR

Study 2: Placebo (cornstarch)-ingestion before and after BFR-exercise

Dietary Supplement: NMN-BFR exercise

NMN-BFR exercise

EXPERIMENTAL

Study 2: NMN-ingestion before and after BFR-exercise

Dietary Supplement: NMN-BFR exercise

Placebo-resistance exercise

PLACEBO COMPARATOR

Study 3: Cornstarch-ingestion before and after resistance exercise

Dietary Supplement: Vitamin C-resistance exercise

Vitamin C-resistance exercise

EXPERIMENTAL

Study 3: Vitamin C-ingestion before and after BFR-exercise

Dietary Supplement: Vitamin C-resistance exercise

Interventions

BFR (180 mmHg) BEHAVIORAL

Sprinting exercise after BFR

Also known as: Sham (20 mmHg)

BFR (180 mmHg); Sham (20 mmHg)

NMN-BFR exercise DIETARY_SUPPLEMENT

NMN ingested before and after BFR exercise

Also known as: Placebo-BFR exercise

NMN-BFR exercise; Placebo-BFR exercise

Vitamin C-resistance exercise DIETARY_SUPPLEMENT

Vitamin C ingested before and after resistance exercise

Also known as: Placebo-resistance exercise

Placebo-resistance exercise; Vitamin C-resistance exercise

Eligibility Criteria

• Age: 20 Years - 30 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy men

You may not qualify if:

• inflammatory or immune conditions

Sponsors & Collaborators

• University of Taipei: lead

Study Sites (1)

Laboratory of Exercise Biochemistry

Taipei, Taiwan, 111, Taiwan

Location

MeSH Terms

Conditions

Motor Activity

Interventions

salicylhydroxamic acid

Condition Hierarchy (Ancestors)

Behavior

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Model Details: Three studies will be conducted: Study 1: 2-arm Study 2: 2-arm Study 3: 2-arm

Study Record Dates

• First Submitted: April 7, 2026
• First Posted: May 6, 2026
• Study Start: August 1, 2022
• Primary Completion: May 1, 2025
• Study Completion: May 1, 2025
• Last Updated: May 6, 2026

Record last verified: 2026-05

Data Sharing

• IPD Sharing: Will not share

Locations

TW (1)