Diode Laser and S100A8 in Peri-Implantitis

NCT07524153 | Completed

• 1 other identifier: 2024.12.21
• Study Type: observational
• Target: 39
• Locations: 1 country
• Sites: 1

Brief Summary

Purpose: The aim of this study was to compare the effectiveness of diode laser and combined treatment protocols, applied in addition to mechanical debridement, in the treatment of peri-implantitis on clinical periodontal parameters and S100A8 levels in peri-implant sulcus fluid (PISF). Methods: A total of 39 patients diagnosed with peri-implantitis were divided into three groups based on the treatment protocols used: Mechanical Debridement, Diode Laser, and Combined Treatment. Probing depth, clinical attachment level, modified plaque index, gingival index, and PISF volume measurements were recorded at baseline and at weeks 4 and 12. S100A8 levels in PISF samples were measured by ELISA.

Conditions

Periimplant Diseases; S100A8; Calprotectin; Biomarkers

Keywords

calprotectin; biomarkers; inflammation; Peri-implantitis

Outcome Measures

Primary Outcomes (1)

• Change in S100A8 levels in Peri-Implant Sulcular Fluid (PISF)

  S100A8 levels will be measured using an ELISA kit (ng/mL) to evaluate the inflammatory response.

  Baseline (Day 0), 28 days (Week 4), and 84 days (Week 12)

Secondary Outcomes (4)

• Probing Depth (PD)

  Baseline (Day 0), 28 days (Week 4), and 84 days (Week 12)

• Clinical Attachment Level (CAL)

  Baseline (Day 0), 28 days (Week 4), and 84 days (Week 12)

• Modified Plaque Index (mPI)

  Baseline (Day 0), 28 days (Week 4), and 84 days (Week 12)

• Gingival Index (GI)

  Baseline (Day 0), 28 days (Week 4), and 84 days (Week 12)

Study Arms (3)

Mechanical Debridement Group

13 participants. Procedure: Subgingival mechanical debridement using sterile titanium curettes to remove biofilm and deposits from the implant surfaces. Collection of PISF samples.

Diode Laser Monotherapy Group

13 participants. Procedure: Decontamination using a 940 nm diode laser (2.5 W, CP2 mode) to remove biofilm from the implant surfaces. Collection of PISF samples.

Combined Group

13 participants. Procedure: Sequential application of subgingival mechanical debridement followed by 940 nm diode laser decontamination. Collection of PISF samples.

Eligibility Criteria

• Age: 30 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

The study population consists of systemically healthy, non-smoking individuals aged 30-65 years who have at least one functional dental implant diagnosed with peri-implantitis (probing depth ≥ 6 mm, no clinical mobility). Participants are selected from patients admitted to the Kırıkkale University Faculty of Dentistry, Department of Periodontology for routine follow-up or treatment.

You may qualify if:

• Individuals aged 30-65 years.
• Presence of at least one functional dental implant with no clinical mobility
• Diagnosis of peri-implantitis with a probing depth (PD) of ≥ 6 mm.
• Ability to provide informed consent and attend follow-up visits.

You may not qualify if:

• Systemic diseases that may interfere with wound healing (e.g., uncontrolled diabetes).
• History of radiation or chemotherapy.
• Pregnancy or lactation.
• Smoking or alcohol consumption.
• Parafunctional habits (e.g., bruxism).
• Use of systemic antibiotics within the last 6 months.
• Periodontal or peri-implant treatment in the relevant area within the last 3 months.

Sponsors & Collaborators

• Kırıkkale University: lead

Study Sites (1)

Kırıkkale University, Faculty of Dentistry

Kırıkkale, Kırıkkale, 71100, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Inflammation; Peri-Implantitis

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms; Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases

Study Officials

• Akif Nalbant, Specialist in Periodontology

  Kirikkale University Faculty of Dentistry

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: DDS

Study Record Dates

• First Submitted: April 1, 2026
• First Posted: April 13, 2026
• Study Start: January 1, 2025
• Primary Completion: June 1, 2025
• Study Completion: October 10, 2025
• Last Updated: April 13, 2026

Record last verified: 2026-04

Locations

TU (1)