Detection of Disease Marker Factors in Cyst Fluid
Measurement of MMP-8, MMP-9, RANKL, MIP-1alpha in Biopsy Samples From Odontogenic Cyst Patients
1 other identifier
observational
25
1 country
1
Brief Summary
The purpose of this observational study is to examine the cyst fluid obtained after a puncture procedure performed for cyst diagnosis in participants with cystic lesions on clinical and radiological examination.The main question it aims to answer is: \- What is the exact mechanism of formation of odontogenic cysts? Biomarkers in the cyst fluid obtained after a puncture procedure performed as part of the diagnosis and treatment process of odontogenic cysts will be examined. The aim of this study is to gain insight into cyst pathology by examining the levels of biomarkers in odontogenic cyst fluid.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for all trials
Started Aug 2025
Shorter than P25 for all trials
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
August 25, 2025
CompletedStudy Start
First participant enrolled
August 25, 2025
CompletedFirst Posted
Study publicly available on registry
September 12, 2025
CompletedPrimary Completion
Last participant's last visit for primary outcome
December 1, 2025
CompletedStudy Completion
Last participant's last visit for all outcomes
May 1, 2026
CompletedSeptember 12, 2025
September 1, 2025
3 months
August 25, 2025
September 9, 2025
Conditions
Keywords
Outcome Measures
Primary Outcomes (4)
MMP-8 concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the MMP-8 concentration level will be measured.
through study completion, an average of 6 months
MMP-9 concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the MMP-9 concentration level will be measured.
through study completion, an average of 6 months
RANKL concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the RANKL concentration level will be measured.
through study completion, an average of 6 months
MIP-1alpha concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the MIP-1alpha concentration level will be measured.
through study completion, an average of 6 months
Secondary Outcomes (1)
Correlation between the type of cyst and the measured markers
through study completion, an average of 6 months
Study Arms (1)
individuals with odontogenic cysts
The cyst fluid obtained during the puncture will be collected in a tube and centrifuged. The clear liquid remaining on top will be transferred to an Eppendorf tube and stored at -80 degrees. After all samples are collected, they will be analyzed using the ELISA method.
Interventions
Participants will not undergo any procedures outside of the routine for the study. The cyst fluid obtained during the puncture will be collected in a tube and centrifuged at 3000 rpm for 20 minutes at 4 degrees Celsius. The Eppendorf tubes will be stored at -80 °C until the clear liquid remaining above is taken and examined, without removing the particles that have settled at the bottom of the tube. The biochemical parameters of the cyst fluid obtained from the study participants will be analyzed using the ELISA method. MMP-8, MMP-9, RANKL, and MIP1alpha will be analyzed using commercially available ELISA kits, following the manufacturer's instructions.
Eligibility Criteria
The study group will consist of individuals aged 12-80 with cystic lesions who agree to participate in the study themselves and with their families (if children), and who are not pregnant or breastfeeding.
You may qualify if:
- Individuals diagnosed with cysts
- Individuals who are not pregnant or breastfeeding
- Individuals who agree to sign the informed consent form
You may not qualify if:
- Systemically ill individuals who are contraindicated for surgery.
- Individuals who do not wish to sign the informed consent form.
- Individuals who are pregnant, breastfeeding, or using oral contraceptives will be excluded.
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
Harran University, Faculty of Dentistry
Sanliurfa, Turkey (Türkiye)
Biospecimen
Cyst fluid obtained during puncture
MeSH Terms
Conditions
Condition Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
hande has elci
Harran University
Central Study Contacts
Study Design
- Study Type
- observational
- Observational Model
- OTHER
- Time Perspective
- PROSPECTIVE
- Target Duration
- 2 Weeks
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Assistant Professor
Study Record Dates
First Submitted
August 25, 2025
First Posted
September 12, 2025
Study Start
August 25, 2025
Primary Completion
December 1, 2025
Study Completion
May 1, 2026
Last Updated
September 12, 2025
Record last verified: 2025-09