Influence of Hydration Status on the Yield of Injectable Platelet-Rich Fibrin

NCT07506252 | Completed

• 1 other identifier: 34-508 ex 21/22
• Study Type: interventional
• Target: 26
• Locations: 1 country
• Sites: 1

Brief Summary

Due to the current lack of evidence regarding the influence of fluid intake on the amount of leukocyte- and platelet-rich fibrin fraction present in blood samples, this study aims to investigate the positive influence of this factor on the body's own matrix. Platelet-rich fibrin, a fibrin-rich autologous matrix obtained from autologous blood via centrifugation, contains not only fibrin but also important growth factors and a large number of white blood cells, all of which make a significant contribution to tissue regeneration. The obtained fibrin matrix can be used as a membrane, as a clot (A-PRF), or as a liquid (i-PRF) to positively influence the healing process. The novelty of this work lies in shifting the focus from purely technical centrifugation parameters to a patient-centered, easily modifiable biological variable. Hydration status represents a low-cost, non-invasive, and immediately applicable strategy to optimize autologous blood concentrates. These findings are directly relevant to clinicians seeking to enhance the predictability and efficiency of PRF-based regenerative procedures in implant dentistry.

Conditions

PRF; Behavior Change Interventions; Implant Dentistry

Keywords

PRF; Hydration; implant dentistry; regenerative dentistry

Outcome Measures

Primary Outcomes (1)

• PRF-Volume

  Volume (ml) of i-PRF obtained from each 10 ml blood sample with low speed centrifugation

  3 visits, 3 weeks

Secondary Outcomes (2)

• PRF yield and height

  3 visits, 3 weeks

• PRF yield and weight

  3 visits, 3 weeks

Study Arms (1)

Hydration

EXPERIMENTAL

Drinking protocol to reach euhydration

Behavioral: Hydration following a drinking protocol

Interventions

Hydration following a drinking protocol BEHAVIORAL

Participants followed a drinking protocol the day before the measurement to reach euhydration

Hydration

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• \- Healthy adults

You may not qualify if:

• coagulation disorders
• anticoagulant medication
• pregnancy
• acute infections
• systemic diseases with the potential to affect wound healing or blood composition

Sponsors & Collaborators

• Medical University of Graz: lead

Study Sites (1)

Medical University of Graz

Graz, Styria, 8010, Austria

Location

Related Publications (2)

• Verboket RD, Anbar B, Sohling N, Kontradowitz K, Marzi I, Ghanaati S, Henrich D. Changes in platelet-rich fibrin composition after trauma and surgical intervention. Platelets. 2020 Nov 16;31(8):1069-1079. doi: 10.1080/09537104.2020.1714575. Epub 2020 Jan 23.

  PMID: 31971046 BACKGROUND

• Hauser F, Gaydarov N, Badoud I, Vazquez L, Bernard JP, Ammann P. Clinical and histological evaluation of postextraction platelet-rich fibrin socket filling: a prospective randomized controlled study. Implant Dent. 2013 Jun;22(3):295-303. doi: 10.1097/ID.0b013e3182906eb3.

  PMID: 23644909 BACKGROUND

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SEQUENTIAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 17, 2026
• First Posted: April 1, 2026
• Study Start: December 12, 2022
• Primary Completion: May 19, 2023
• Study Completion: May 19, 2023
• Last Updated: April 1, 2026

Record last verified: 2026-03

Locations

AU (1)