Efficacy and Safety of Combined Nanofat Injection With Either Platelet Rich Fibrin or Microneedling Versus Nanofat Injection Alone in the Treatment of Facial Atrophic Post Acne Scars

NCT07471854 | Not Yet Recruiting

• 1 other identifier: Nanofat in post acne scars
• Study Type: interventional
• Target: 42
• Locations: 0 countries
• Sites: N/A

Brief Summary

To compare the efficacy and safety of combined nanofat injection with either platelet rich fibrin or microneedling versus nanofat injection alone in the treatment of facial atrophic post acne scars.

Conditions

Acne Scars - Atrophic

Outcome Measures

Primary Outcomes (1)

• compare the efficacy and safety of combined nanofat injection with either platelet rich fibrin or microneedling versus nanofat injection alone in the treatment of facial atrophic post acne scars.

  Comaprison among different treatment modalities will be done by 1-Goodman and Baron qualitative score : Goodman and Baron qualitative Scale for each side of the face will be performed for all groups by counting and grading scars at the baseline and 3 months after the last session, then comparing its values before the start of treatment and 3 months after the last session Grade 1 (Macular): Erythematous, hyperpigmented, or hypopigmented flat marks. Grade 2 (Mild): Mild atrophic or hypertrophic scars, usually concealable with makeup or hair. Grade 3 (Moderate): Obvious, noticeable scars that are not easily hidden, sometimes showing "ice-pick" characteristics. Grade 4 (Severe): Marked, deep, or severe scarring, or widespread, severe scarring. Is nanofat injection alone effective in treamtment of post acne scars or when combined with either platelt rich fibrin or microneedling is better?

  6 months

Secondary Outcomes (3)

• Patient's satisfaction

  6 months

• Pain assessment

  6 months

• Histopathological evaluation

  6 months

Study Arms (2)

nanofat injection and platelet rich fibrin

ACTIVE COMPARATOR

(21 patients) single nanofat injection on both sides of the face, followed by platelet rich fibrin injection on one side of face monthly for 3 months.

Procedure: nanofat injecion; Procedure: Platelet rich fibrin

nanofat injection and miconeedling

ACTIVE COMPARATOR

(21 patients)- single nanofat injection on both sides of the face, followed by microneedling on one side of face monthly for 3 months.

Procedure: nanofat injecion; Procedure: Microneedling

Interventions

nanofat injecion PROCEDURE

potential donor sites for fat graft including the lower abdomen, flanks, hips and thighs. After injection of 2 cm of lidocaine hydrochloride 2% intradermally at site of entry blade will be stabbed to open access for harvesting cannula to access subcutaneous fat. Tumescent anesthesia will be infiltrated in multidirectional plane to numb the whole area and waiting for 20 minutes, then starting to push the harvesting cannula in and out with slight suction pressure created by pulling the plunger of 20 ml luer-lock syringe, finally subcutaneous fat started to be expelled in the syringe The aspirated fat will be centrifuged at 3000 rpm for 3 min to concentrate fat particles Then the lipoaspirate will be mechanically emulsified using 2.4, 1.4, and 1.2 mm Luer-to-Luer Tulip connectors via 30 mechanical passes between two syringes through each connector, respectively A filtration process will be performed using a special filter containing a mesh with ultra-fine holes to remove debris

nanofat injection and miconeedling; nanofat injection and platelet rich fibrin

Platelet rich fibrin PROCEDURE

PRF will be produced by single spin centrifugation of 10 ml of venous blood collected in plain glass tube without anticoagulant at 700 rpm for 3 min. The upper layer, yellow to orange colored fluid, will be collected as fluid PRF. Approximately, 1 ml Fluid PRF can be separated from 10 ml blood. * 45 min before the session, local anesthetic cream containing mixture of lidocaine and prilocaine will be applied to one side of the face, then will be sterilized by alcohol before starting the treatment. * one side of face will be treated by intradermal injection of fluid PRF. 0.1 mL of fluid PRF will be injected intradermally into the atrophic scars with 1.5 to 2 cm interval using insulin syringe followed by gentle massaging of the treated area. * The treatment will be started immediately after separation of PRF to avoid clot formation. The patients will receive three treatment sessions with 4 weeks interval

nanofat injection and platelet rich fibrin

Microneedling PROCEDURE

Microneedling will be done by Derma electric-pen, and needle cartridge with 12 needles. Needle length will be adjusted at 2.5 mm and speed level 4 (blue color). * 45 min before the session, local anesthetic cream containing mixture of lidocaine and prilocaine will be applied to one side of the face, then will be sterilized by alcohol before starting the treatment. Dermapen will be moved in the four directions, vertically, horizontally, diagonally right and left, over one side of the face without pressing. * The patients will receive three treatment sessions with 4 weeks interval

nanofat injection and miconeedling

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Patients older than 18 years of both sexes with moderate to severe atrophic facial acne scars on both cheeks

You may not qualify if:

• \. Patients' age \<18 or \>45 years 2. Pregnant and lactating women 3. active inflammatory acne or active infection in the treatment area. 4. treatment of the targeted study sites within 2 months 5. history of chronic diseases (autoimmune diseases, bleeding disorders, taking anticoagulant therapy or aspirin) 6. history of hypertrophic scars or keloid formation tendency. 7. patients with unrealistic expectations.

Sponsors & Collaborators

• Assiut University: lead

Related Publications (15)

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• Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009 Mar;27(3):158-67. doi: 10.1016/j.tibtech.2008.11.009. Epub 2009 Jan 31.

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• Rageh MA, El-Khalawany M, Ibrahim SMA. Autologous nanofat injection in treatment of scars: A clinico-histopathological study. J Cosmet Dermatol. 2021 Oct;20(10):3198-3204. doi: 10.1111/jocd.14363. Epub 2021 Aug 6.

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MeSH Terms

Interventions

Percutaneous Collagen Induction

Intervention Hierarchy (Ancestors)

Complementary Therapies; Therapeutics; Physical Therapy Modalities; Punctures; Rehabilitation

Study Officials

• Radwa Mohamed Abdel Moniem Bakr, Associate professor

  STUDY DIRECTOR

• Howida Omar Mahmoud, Associate professor

  STUDY DIRECTOR

• Eman Mohamed Kamal Elsayed Youssef, Professor

  unafflilted

  STUDY DIRECTOR

Central Study Contacts

Marilyn Sameh Abd Almalak, Assistant lecturer

CONTACT

+201226310521; marilyn.sameh@aun.edu.eg

Eman Mohamed Kamal Elsayed Youssef, Professor

CONTACT

+201005369338; emankyoussef@aun.edu.eg

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer

Model Details: Nanofat injection

Study Record Dates

• First Submitted: March 9, 2026
• First Posted: March 13, 2026
• Study Start: May 1, 2026
• Primary Completion (Estimated): May 1, 2027
• Study Completion (Estimated): December 1, 2027
• Last Updated: March 18, 2026

Record last verified: 2026-03