mexB Efflux Pump Gene Expression, Activity and Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa

NCT07469436 | Not Yet Recruiting

• 1 other identifier: RBMEPGEEPAABFIMRCIOPA
• Study Type: observational
• Target: 80
• Locations: 0 countries
• Sites: N/A

Brief Summary

1. 1.Detection of expression level of mexB efflux pump gene in multidrug resistant clinical isolates of Pseudomonas aeruginosa by real time PCR(QRT-PCR).
2. 2.Phenotypic detection of efflux pump activity in multidrug resistant clinical isolates of Pseudomonas aeruginosa.
3. 3.Phenotypic detection of biofilm formation and its degree (strong-moderate-weak) in multidrug resistant clinical isolates of Pseudomonas aeruginosa.
4. 4.Relationship between expression level of mexB efflux pump gene and degree of biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa.

Conditions

Pseudomonas Aeroginosa

Outcome Measures

Primary Outcomes (1)

• Detection of mexB efflux pump gene expression level in multidrug resistant clinical isolates of Pseudomonas aeruginosa

  molecular detection of mexB efflux pump gene expression level by real time PCR (QRT-PCR)

  one year

Secondary Outcomes (2)

• Detection of efflux pump activity in multidrug resistant clinical isolates of Pseudomonas aeruginosa

  one year

• Detection of degree of biofilm formation (strong-moderate-weak) in multidrug resistant clinical isolates of Pseudomonas aeruginosa

  one year

Interventions

Real time PCR GENETIC

Clinical isolates of Pseudomonas aeruginosa will undergo RNA extraction followed by cDNA (complementary DNA) synthesis. Quantitative real-time PCR will be performed using specific primers for the mexB efflux pump gene, and SYBR Green will be used to quantify gene expression. Housekeeping genes (e.g., rpoD or 16S rRNA) will serve as internal controls. The procedure allows precise quantification of mexB expression levels in each isolate.

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients with multidrug resistant clinical isolates of Pseudomonas aeruginosa.

You may qualify if:

• Clinical isolates confirmed as Pseudomonas aeruginosa by microbiological testing.
• Isolates collected from different clinical specimens (e.g., wound swabs, sputum, urine, blood, and catheters).

You may not qualify if:

• Bacterial species other than Pseudomonas aeruginosa.
• Repeated clinical isolates from the same patient.
• Repeated clinical specimens from different sources of the same patient.

Sponsors & Collaborators

• Assiut University: lead

Related Publications (9)

• Li XF, Shi HQ, Liang Y, Li J, Jiang B, Song GB. Interaction of biofilm and efflux pump in clinical isolates of carbapenem resistant P. aeruginosa. Eur Rev Med Pharmacol Sci. 2022 Mar;26(5):1729-1737. doi: 10.26355/eurrev_202203_28242.

  PMID: 35302222 BACKGROUND

• Soltani Borchaloee A, Moosakazemi Mohammadi LS, Khosh Ravesh R, Allameh SF, Tabatabaie Poya FS, Fatehi MA. Prevalence of Biofilm and Efflux Pump Genes Expression by PCR and Antibiotic Resistance Pattern in Pseudomonas Aeruginosa. Arch Razi Inst. 2024 Dec 31;79(6):1281-1286. doi: 10.32592/ARI.2024.79.6.1281. eCollection 2024 Dec.

  PMID: 40599435 BACKGROUND

• Liao C, Huang X, Wang Q, Yao D, Lu W. Virulence Factors of Pseudomonas Aeruginosa and Antivirulence Strategies to Combat Its Drug Resistance. Front Cell Infect Microbiol. 2022 Jul 6;12:926758. doi: 10.3389/fcimb.2022.926758. eCollection 2022.

  PMID: 35873152 BACKGROUND

• Ugwuanyi FC, Ajayi A, Ojo DA, Adeleye AI, Smith SI. Evaluation of efflux pump activity and biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa isolated from a Federal Medical Center in Nigeria. Ann Clin Microbiol Antimicrob. 2021 Feb 2;20(1):11. doi: 10.1186/s12941-021-00417-y.

  PMID: 33531042 BACKGROUND

• Karballaei Mirzahosseini H, Hadadi-Fishani M, Morshedi K, Khaledi A. Meta-Analysis of Biofilm Formation, Antibiotic Resistance Pattern, and Biofilm-Related Genes in Pseudomonas aeruginosa Isolated from Clinical Samples. Microb Drug Resist. 2020 Jul;26(7):815-824. doi: 10.1089/mdr.2019.0274. Epub 2020 Jan 23.

  PMID: 31976811 BACKGROUND

• Hajiagha MN, Kafil HS. Efflux pumps and microbial biofilm formation. Infect Genet Evol. 2023 Aug;112:105459. doi: 10.1016/j.meegid.2023.105459. Epub 2023 Jun 2.

  PMID: 37271271 BACKGROUND

• Ren J, Wang M, Zhou W, Liu Z. Efflux pumps as potential targets for biofilm inhibition. Front Microbiol. 2024 Feb 23;15:1315238. doi: 10.3389/fmicb.2024.1315238. eCollection 2024.

  PMID: 38596384 BACKGROUND

• Soto SM. Role of efflux pumps in the antibiotic resistance of bacteria embedded in a biofilm. Virulence. 2013 Apr 1;4(3):223-9. doi: 10.4161/viru.23724. Epub 2013 Feb 4.

  PMID: 23380871 BACKGROUND

• Habib MB, Shah NA, Amir A, Alghamdi HA, Tariq MH, Nisa K, Ammoun M. Decoding MexB efflux pump genes: structural, molecular, and phylogenetic analysis of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa. Front Cell Infect Microbiol. 2025 Jan 21;14:1519737. doi: 10.3389/fcimb.2024.1519737. eCollection 2024.

  PMID: 39906216 BACKGROUND

MeSH Terms

Interventions

Real-Time Polymerase Chain Reaction

Intervention Hierarchy (Ancestors)

Polymerase Chain Reaction; Nucleic Acid Amplification Techniques; Genetic Techniques; Investigative Techniques

Study Officials

• Assiut University

  Assiut University

  STUDY DIRECTOR

Central Study Contacts

Rehab AM Omran

CONTACT

01025113833; remy25.1994rn@gmail.com

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer

Study Record Dates

• First Submitted: March 5, 2026
• First Posted: March 13, 2026
• Study Start: April 1, 2026
• Primary Completion (Estimated): December 1, 2027
• Study Completion (Estimated): January 1, 2028
• Last Updated: March 17, 2026

Record last verified: 2026-03

Data Sharing

• IPD Sharing: Will not share