NCT07457398

Brief Summary

This study investigates the acute and 24-hour recovery effects of localized muscle fatigue on the viscoelastic properties of the medial gastrocnemius (MG) muscle and vertical jump performance. The research aims to quantify the changes in muscle stiffness, tone, and elasticity immediately after a standardized fatigue protocol and to monitor the recovery of these parameters 24 hours later. Additionally, the study evaluates the impact of fatigue on explosive power, measured by countermovement jump (CMJ) height, across these time points.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
28

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jan 2026

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 15, 2026

Completed
1 month until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 15, 2026

Completed
9 days until next milestone

Study Completion

Last participant's last visit for all outcomes

February 24, 2026

Completed
1 day until next milestone

First Submitted

Initial submission to the registry

February 25, 2026

Completed
12 days until next milestone

First Posted

Study publicly available on registry

March 9, 2026

Completed
Last Updated

May 22, 2026

Status Verified

May 1, 2026

Enrollment Period

1 month

First QC Date

February 25, 2026

Last Update Submit

May 19, 2026

Conditions

Keywords

Muscle FatigueMuscle Myotonometric PropertiesCountermovement Jump

Outcome Measures

Primary Outcomes (1)

  • Muscle Stiffness of the Medial and Lateral Gastrocnemius

    Evaluation of the intrinsic mechanical stiffness of the medial (MG) and lateral (LG) gastrocnemius muscles using myotonometry. Higher values indicate greater stiffness. Measured in Newtons per meter (N/m).

    Baseline, immediately post-fatigue (Post-0), and 24 hours post-fatigue (Post-24h)

Secondary Outcomes (3)

  • Vertical Jump Performance (Jump Height)

    Baseline, immediately post-fatigue (Post-0), and 24 hours post-fatigue (Post-24h)

  • Countermovement Jump Phase Durations (Braking and Eccentric)

    Baseline, immediately post-fatigue (Post-0), and 24 hours post-fatigue (Post-24h)

  • Dynamic Balance Performance (Y-Balance Test)

    Baseline, immediately post-fatigue (Post-0), and 24 hours post-fatigue (Post-24h)

Study Arms (1)

Gastrocnemius Fatigue Group

EXPERIMENTAL

Participants in this arm undergo a standardized localized muscle fatigue protocol for the plantar flexors. This involves performing repetitive standing calf raises at a constant tempo of 60 beats per minute until task failure. Muscle myotonometric properties (tone, stiffness, elasticity) and vertical jump performance (CMJ) are measured at baseline, immediately post-fatigue, and 24 hours later to evaluate acute changes and recovery.

Device: Isometric Plantar Flexion Fatigue Protocol

Interventions

Participants undergo a localized isometric fatigue protocol in a supine position with their feet placed against a solid wall. They perform maximal isometric plantar flexion pushing against the wall. The protocol continues until the participant reports a score of 8 or higher (indicating 'very strong' to 'maximal' exertion) on the Borg CR-10 Scale. Task failure is also confirmed by the inability to maintain the required isometric pressure or foot position despite verbal encouragement. This ensures a standardized level of subjective and objective muscle fatigue across all participants.

Gastrocnemius Fatigue Group

Eligibility Criteria

Age18 Years - 25 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy volunteers aged between 18 and 25.
  • Physical activity level categorized as sedentary or recreationally active according to IPAQ.
  • Willingness to follow the supine isometric fatigue protocol and attend follow-up measurements.
  • Sedentary or recreationally active (IPAQ scores).

You may not qualify if:

  • History of lower extremity musculoskeletal injuries or surgeries within the last 6 months.
  • Any diagnosed neurological, metabolic, or cardiovascular diseases.
  • Regular use of medications or supplements that may affect muscle tone or recovery (e.g., muscle relaxants, high-dose antioxidants).
  • Recent history of intensive strength training for the lower limbs.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Istanbul Beykent University

Istanbul, Turkey (Türkiye)

Location

Study Officials

  • Ali İlez, PhD Can

    VU University of Amsterdam

    PRINCIPAL INVESTIGATOR
  • Nergiz Batur, PhD Can

    Galata University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Masking Details
Masking is not possible due to the nature of the physical fatigue protocol.
Purpose
OTHER
Intervention Model
SINGLE GROUP
Model Details: A single-group, repeated measures design where all participants undergo a fatigue protocol, with measurements taken at three time points: baseline, immediately post-fatigue, and 24 hours later.
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant Professor

Study Record Dates

First Submitted

February 25, 2026

First Posted

March 9, 2026

Study Start

January 15, 2026

Primary Completion

February 15, 2026

Study Completion

February 24, 2026

Last Updated

May 22, 2026

Record last verified: 2026-05

Data Sharing

IPD Sharing
Will not share

Individual participant data will not be publicly available to protect participant privacy. However, de-identified aggregate data and study protocols may be shared upon reasonable request to the corresponding author.

Locations