Whey Protein Supplementation and Serum Mineral Status in Elite Endurance and Power Athletes

NCT07488273 | Completed DMC

• 1 other identifier: PNU-12
• Study Type: interventional
• Target: 59
• Locations: 1 country
• Sites: 1

Brief Summary

This study investigates whether 8 weeks of post-training whey protein supplementation (30 g/day) affects serum mineral concentrations (calcium, phosphorus, magnesium, and iron) in elite endurance and power athletes during structured training.

Conditions

Athletic Performance

Keywords

Athletic Performance; Mineral Homeostasis

Outcome Measures

Primary Outcomes (1)

• serum mineral concentrations (Calcium, Phosphorus, Magnesium, Iron)

  Serum concentrations of calcium, phosphorus, magnesium, and iron will be measured using standardized enzymatic and colorimetric assays from venous blood samples collected at rest. Measurements will be performed at baseline and after the intervention to assess changes associated with training and whey protein supplementation in elite athletes.

  Baseline (Week 1) and Post-Intervention (Week 10)

Study Arms (2)

Whey Protein Group (WG)

EXPERIMENTAL

Receives 30 g/day whey protein post-training for 8 weeks

Dietary Supplement: Whey Protein Supplementation

Control Group (CG)

OTHER

Continues usual training without supplementation

Other: No Supplement (Control)

Interventions

Whey Protein Supplementation DIETARY_SUPPLEMENT

Participants in the intervention group consumed 30 g/day of whey protein powder within 30 minutes after training sessions for 8 weeks as part of a structured training program. The supplement (IronMaxx®, 100% Whey Protein) primarily consisted of whey protein concentrate and provided approximately 24 g of protein per daily dose. The intervention was administered in an open-label manner without placebo control. Participants were instructed to maintain their habitual diet and refrain from using additional nutritional supplements during the study period.

Also known as: Whey protein powder, IronMaxx 100% Whey Protein

Whey Protein Group (WG)

No Supplement (Control) OTHER

Participants continued their usual sport-specific training program for 8 weeks without receiving whey protein or any additional nutritional supplementation. They were instructed to maintain their habitual diet and avoid initiating any new supplements during the study period.

Also known as: Usual training only

Control Group (CG)

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male elite track-and-field athletes
• Age 18-30 years
• Engaged in structured training program
• No supplement use in past 3 months

You may not qualify if:

• Use of nutritional or mineral supplements
• Metabolic or musculoskeletal disorders
• Acute injury affecting performance
• Use of ergogenic aids (e.g., creatine, anabolic agents)

Sponsors & Collaborators

• Monira Aldhahi: lead

Study Sites (1)

Training Centers

Tunis, Tunisia

Location

MeSH Terms

Interventions

Whey Proteins

Intervention Hierarchy (Ancestors)

Milk Proteins; Animal Proteins, Dietary; Dietary Proteins; Proteins; Amino Acids, Peptides, and Proteins; Whey; Milk; Dairy Products; Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Associate Prof

Model Details: Participants are divided into whey supplementation and control groups within endurance and power disciplines, with no randomization or blinding.

Study Record Dates

• First Submitted: March 17, 2026
• First Posted: March 23, 2026
• Study Start: February 25, 2026
• Primary Completion: February 26, 2026
• Study Completion: March 15, 2026
• Last Updated: March 24, 2026

Record last verified: 2026-03

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)