Linking Dietary Nutrition, Dietary Patterns, Gut Microbiota, and Bone Density: A Cross-Sectional Study

NCT07256314 | Completed

• 1 other identifier: IRB23082901
• Study Type: observational
• Target: 431
• Locations: 1 country
• Sites: 1

Brief Summary

The study aims to understand the current situation of bone health in the Gannan Tibetan primary school student population, to explore the relationship between nutrition, intestinal microorganisms and bone health, to analyze the main factors affecting bone health from the perspective of nutrition and intestinal microecology, and to provide theoretical basis for the promotion and improvement of bone health in Tibetan primary school students.

Conditions

Bone Density; Gut Microbiomes

Outcome Measures

Primary Outcomes (3)

• Dietary Characteristics

  Dietary data were collected using a food frequency questionnaire (FFQ) to analyze dietary patterns and nutrient intake.

  Day 1

• Bone density test

  In this study, a peripheral dual-energy X-ray absorptiometry (EXA-PRESTO) was used to measure the bone density of the non-dominant forearm of the subjects.

  Day1

• Fecal sample microbiological testing and analysis

  This study entrusted Tianhao Gene Technology Co., Ltd. to perform the intestinal microbiome Accu16STM (16S rRNA absolute quantitative sequencing). The main research processes include DNA extraction, PCR amplification, library construction, and sequencing.

  Day 1

Study Arms (1)

Good Bone Density group and normal bone density group

Other: No Intervention: Observational Cohort

Interventions

No Intervention: Observational Cohort OTHER

No Intervention

Good Bone Density group and normal bone density group

Eligibility Criteria

• Age: 6 Years - 13 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)
• Sampling Method: Probability Sample

Study Population

This study is based on a cross-sectional design. Two schools were selected: one rural primary school in Xiahe County, Gannan Tibetan Autonomous Prefecture, and one urban primary school in Hezuo City, Gansu Province. Within the selected schools, a stratified random cluster sampling method was used to randomly select one class from each grade as the study subjects.

You may qualify if:

• Tibetan primary school students currently enrolled in schools in Gannan Tibetan Autonomous Prefecture;
• Willing to participate and able to cooperate in completing bone density measurements;

You may not qualify if:

• Suffering from skeletal system diseases, metabolic disorders, or endocrine diseases;

Sponsors & Collaborators

• Jianhua Ma: lead

Study Sites (1)

Lanzhou university

Lanzhou, Gansu, 730000, China

Location

Biospecimen

Retention: SAMPLES WITH DNA

Faecal samples

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Lecturer

Study Record Dates

• First Submitted: November 20, 2025
• First Posted: December 1, 2025
• Study Start: October 10, 2023
• Primary Completion: March 31, 2025
• Study Completion: March 31, 2025
• Last Updated: December 5, 2025

Record last verified: 2025-11

Data Sharing

• IPD Sharing: Will not share

Locations

CN (1)