Acute Effects of High Impact Exercise on Bone Metabolism

NCT07245225 | Active Not Recruiting

• 1 other identifier: 16204
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this study is to investigate the acute effects of drop-landing exercise on the bone metabolism biomarkers and extracellular vesicles in healthy young males, in order to gain a deeper understanding of the mechanotransduction mechanisms involved in bone metabolism. The main question aims to answer: • Does a single bout acute drop-landing exercise change serum sclerostin, other bone signaling markers, and circulating extracellular vesicles levels? Participants complete both the drop-landing and control trials in a randomized order, with a minimum washout period of one week between trials. During each trial, blood samples are collected at three time points: pre-, immediately post, and 1-hour post drop-landing/control exercise.

Conditions

Health Adults

Keywords

Exercise; Bone turnover; Extracellular vesicles; Wnt/β-catenin signaling pathway

Outcome Measures

Primary Outcomes (1)

• Serum sclerostin concentration

  Serum sclerostin concentration is quantified using an enzyme-linked immunosorbent assay (ELISA) and reported in picograms per milliliter (pg/mL).

  Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials.

Secondary Outcomes (5)

• Serum dickkopf Wnt signaling pathway inhibitor 1 (DKK1) concentration

  Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials.

• Serum osteoprotegerin (OPG) concentration

  Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials.

• Serum irisin concentration

  Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials.

• Circulating extracellular vesicle (EV) particle concentration and size

  Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials.

• Ground reaction force during drop-landing exercise

  Ground reaction force is recorded during each drop-landing exercise.

Study Arms (2)

Arm Drop-landing + Control

EXPERIMENTAL

According to the results of random allocation, participants first perform the drop-landing trial, followed by the control trial, with a minimum interval of one week between the two trials.

Behavioral: Exercise

Arm Control + Drop-landing

EXPERIMENTAL

According to the results of random allocation, participants first perform the control trial, followed by the drop-landing trial, with a minimum interval of one week between the two trials.

Behavioral: Exercise

Interventions

Exercise BEHAVIORAL

During the drop-landing trial, participants perform drop-landing exercises from a block calibrated to generate approximately four times their body weight in vertical ground reaction force upon landing, employing a stiff-landing technique. Each repetition is separated by a 30-second interval, and 10 repetitions are performed as 1 set, totalling 6 sets. There is a 2-minute rest break between each set, during which the participants are asked to sit on a chair. The entire drop-landing exercise is recorded on the force plate. During the control trial, participants are required to walk up and down from the same height block without the requirement to perform the drop-landing movement. The number of repetitions, intervals, and rest breaks are completely consistent with the drop-landing exercise.

Arm Control + Drop-landing; Arm Drop-landing + Control

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• years old
• Healthy
• Male
• Body mass index (BMI) of 18.5-29.9
• Can understand and follow trial instructions

You may not qualify if:

• Have any uncontrolled cardiovascular or respiratory disease
• Have severe musculoskeletal or neurological disease likely to affect ability to perform an exercise
• Have medical conditions known to affect musculoskeletal health
• Have medications known to affect bone metabolism (e.g., corticosteroids, thyroxine, antiepileptic, and antiretroviral agents)
• Regular (more than once per week) participation in high-intensity resistance or impact-type exercise in the past 6 months
• Current smoker or vaper

Sponsors & Collaborators

• Loughborough University: lead

Study Sites (1)

Loughborough University

Loughborough, Leicestershire, LE11 3TU, United Kingdom

Location

MeSH Terms

Conditions

Motor Activity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Katherine Brooke-Wavell

  Loughborough University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: November 14, 2025
• First Posted: November 24, 2025
• Study Start: January 15, 2024
• Primary Completion: December 31, 2025
• Study Completion: December 31, 2025
• Last Updated: November 24, 2025

Record last verified: 2025-11

Locations

GB (1)