Caffeine Mouth Rinsing at Different Doses in Female Team-Sport Athletes: A Randomized, Double-Blind, Placebo-Controlled Trial

NCT07129863 | Completed

• 1 other identifier: UPC-Comillas
• Study Type: interventional
• Target: 13
• Locations: 1 country
• Sites: 1

Brief Summary

Objective: To evaluate the effects of different caffeine mouth rinse concentrations (1%, 2%, and 3%) on Running Anaerobic Sprint Test (RAST) performance in female team-sport athletes. Main Questions:

1. 1.Do caffeine mouth rinses improve RAST performance in female team-sport athletes?
2. 2.Do different caffeine mouth rinse concentrations affect ratings of perceived exertion (RPE)?

Conditions

Dietary Supplement; Sports Performance

Keywords

carbohydrate; nutrition; mouth rinse; placebo

Outcome Measures

Primary Outcomes (1)

• Running-based anaerobic sprint test (RAST protocol)

  The RAST was comprised of six 35 m sprints at maximum effort, each of which was followed by a 10 s passive rest period (including deceleration phase). Participants rinsed the solution in the final 5 s of the rest period accompanied by the counting of the tester, and then sprint back to the first timing gate with "GO" command. This was repeated until six sprints had been accomplished. Participants began to sprint running for a maximum of one step before the timing gate and were instructed not to decelerate before passing through the timing gate. Using a system of photocells (Newtest Powertimer 300-series, Newtest Oy, Tyrnävä, Finland), which was placed at the beginning and the end of the 35 m, the duration of each 35 m sprint running time was recorded. Using time of each sprint attempt, 1-) fastest sprint time, 2-) mean sprint time, and 3-) fatigue index were recorded

  2-week

Secondary Outcomes (1)

• Rating of perceived exertion (RPE)

  2-weeks

Study Arms (4)

Placebo (water)

PLACEBO COMPARATOR

Placebo: Mouth rinse with plain water, containing no caffeine, serving as the control condition.

Dietary Supplement: Placebo: Mouth rinse with plain water

Low mouth rinse caffeine concentration

EXPERIMENTAL

Low caffeine concentration: Mouth rinse containing 1% caffeine solution (based on weight/volume).

Dietary Supplement: Experimental: Low mouth rinse caffeine concentration (1%)

Moderate mouth rinse caffeine concentration

EXPERIMENTAL

Moderate caffeine concentration: Mouth rinse containing 2% caffeine solution (based on weight/volume).

Dietary Supplement: Moderate caffeine concentration (2% caffeine solution weight/volume).

High mouth rinse caffeine concentration

EXPERIMENTAL

Moderate caffeine concentration: Mouth rinse containing 3% caffeine solution (based on weight/volume).

Dietary Supplement: High caffeine concentration (3% caffeine solution weight/volume).

Interventions

Placebo: Mouth rinse with plain water DIETARY_SUPPLEMENT

Placebo: Mouth rinse with plain water, containing no caffeine, serving as the control condition.

Placebo (water)

Experimental: Low mouth rinse caffeine concentration (1%) DIETARY_SUPPLEMENT

Arm Description: Low caffeine concentration: Mouth rinse containing 1% caffeine solution (based on weight/volume).

Low mouth rinse caffeine concentration

Moderate caffeine concentration (2% caffeine solution weight/volume). DIETARY_SUPPLEMENT

Moderate caffeine concentration: Mouth rinse containing 2% caffeine solution (weight/volume).

Moderate mouth rinse caffeine concentration

High caffeine concentration (3% caffeine solution weight/volume). DIETARY_SUPPLEMENT

High caffeine concentration: Mouth rinse containing 3% caffeine solution (weight/volume).

High mouth rinse caffeine concentration

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female team sports athletes aged between 18 and 35 years.
• Currently engaged in team sports.
• Non-smoker.

You may not qualify if:

• Participants were excluded if they met any of the following conditions.
• Presence of a musculoskeletal injury within the six months prior to the study.
• Current smoker.
• Use of nutritional supplements or ergogenic aids that could influence sprint running performance (e.g., beta-alanine, creatine).
• Use of oral contraceptives.
• Known allergy to caffeine.
• Failure to meet the training requirement of at least six hours of team-based practice, a minimum of three days per week, over the past year.

Sponsors & Collaborators

• Universidad Pontificia Comillas: lead
• Eastern Mediterranean University: collaborator
• Northumbria University: collaborator
• Universidad Francisco de Vitoria: collaborator
• Ankara University: collaborator

Study Sites (1)

Eastern Mediterranean University

Mersin, SBF C 116, Turkey (Türkiye)

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: August 11, 2025
• First Posted: August 19, 2025
• Study Start: September 24, 2023
• Primary Completion: December 1, 2024
• Study Completion: December 22, 2024
• Last Updated: August 19, 2025

Record last verified: 2025-08

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)