NCT07120984

Brief Summary

The purpose of this study is to explore the safety and efficacy of the antibody-cytokine fusion protein L19TNF alone or in combination with alkylating chemotherapy in patients with recurrent IDH mutant glioma.

Trial Health

65
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
52

participants targeted

Target at P25-P50 for phase_2

Timeline
34mo left

Started Jan 2026

Typical duration for phase_2

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress9%
Jan 2026Jan 2029

First Submitted

Initial submission to the registry

July 30, 2025

Completed
14 days until next milestone

First Posted

Study publicly available on registry

August 13, 2025

Completed
6 months until next milestone

Study Start

First participant enrolled

January 30, 2026

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 30, 2029

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 30, 2029

Last Updated

August 13, 2025

Status Verified

July 1, 2025

Enrollment Period

3 years

First QC Date

July 30, 2025

Last Update Submit

August 6, 2025

Conditions

Outcome Measures

Primary Outcomes (1)

  • Progression-free survival (PFS)

    Progression-free survival (PFS) in according to RECIST v.1.1. The duration is defined beginning from randomization to progression or death from any cause.

    The PFS rate will be assessed at 12 months (PFS-12) in IDH mutant astrocytoma and in oligodendroglioma.

Study Arms (7)

1: perioperative cohort of patients with recurrent astrocytoma or oligodendroglioma

EXPERIMENTAL

patients will be treated with 1 cycle (D1, D3 and D5) L19TNF before resection and 4 weeks after surgery

Biological: L19TNF

2A:L19TNF Monotherapy for patients with recurrent oligodendroglioma

EXPERIMENTAL

patients with IDH-mutant oligodendroglioma, WHO Grade 2 or 3 at first progression after radiotherapy and one line of systemic chemotherapy, are treated with up to 6 cycles of 6 weeks with L19TNF

Biological: L19TNF monothery in recurrent oligodendroglioma

2B1: L19TNF plus TMZ for patients with recurrent oligodendroglioma

EXPERIMENTAL

patients with IDH-mutant oligodendroglioma, WHO Grade 2 or 3 at first progression after radiotherapy and one line of systemic CCNU-based chemotherapy, are treated with L19TNF and temozolomide chemotherapy TMZ

Biological: L19TNF and TMZ

2B2: L19TNF plus CCNU for patients with recurrent oligodendroglioma

EXPERIMENTAL

patients with IDH-mutant oligodendroglioma, WHO Grade 2 or 3 at first progression after radiotherapy and one line of systemic temozolomide chemotherapy, are treated with L19TNF and CCNU

Biological: L19TNF and CCNU

3A: L19TNF monotherapy for patients with recurrent astrocytoma

EXPERIMENTAL

patients with IDH-mutant astrocytoma, WHO Grade 2, 3 or 4 at first progression after radiotherapy and one line of systemic chemotherapy, are treated with L19TNF

Biological: L19TNF monotherapy

3B1: L19TNF plus TMZ for patients with recurrent astrocytoma

EXPERIMENTAL

patients with IDH-mutant astrocytoma, WHO Grade 2, 3 or 4 at first progression after radiotherapy and one line of systemic CCNU-based chemotherapy, are treated with L19TNF and temozolomide chemotherapy TMZ

Biological: L19TNF and TMZ in recurrent astrocytoma

3B2: L19TNF plus CCNU for patients with recurrent astrocytoma

EXPERIMENTAL

patients with IDH-mutant astrocytoma, WHO Grade 2, 3 and 4 at first progression after radiotherapy and one line of systemic chemotherapy including temozolomide, are treated with L19TNFand CCNU

Biological: L19TNF and CCNU in recurrent astrocytoma

Interventions

L19TNFBIOLOGICAL

1 cycle of TNF before resection and 6 cycles after surgery

1: perioperative cohort of patients with recurrent astrocytoma or oligodendroglioma
L19TNF and TMZBIOLOGICAL

6 cycles of 28 days with L19TNF and TMZ

2B1: L19TNF plus TMZ for patients with recurrent oligodendroglioma
L19TNF and CCNUBIOLOGICAL

6 cycles of 6 weeks of L19TNF and CCNU every 6 weeks

2B2: L19TNF plus CCNU for patients with recurrent oligodendroglioma

6 cycles of 6 weeks with L19TNF

3A: L19TNF monotherapy for patients with recurrent astrocytoma

6 cycles of 28 days with L19TNF (D1, D3, D5) and temozolomide chemotherapy TMZ (D1-5).

3B1: L19TNF plus TMZ for patients with recurrent astrocytoma

6 cycles of 6 weeks of L19TNF (D1, D3, D5, D22, D24 and D26) and CCNU (D1) every 6 weeks

3B2: L19TNF plus CCNU for patients with recurrent astrocytoma

6 cycles of 6 weeks with L19TNF

2A:L19TNF Monotherapy for patients with recurrent oligodendroglioma

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Age ≥18
  • IDH-mutant glioma (according to WHO 2021 classification) at first recurrence or progression after alkylating chemotherapy:
  • COHORT 1: Grade ≥2 oligodendroglioma or astrocytoma with planned resection, tumor tissue from prior resection must be available
  • COHORT 2A: Grade ≥2 oligodendroglioma
  • COHORT 2B1: Grade ≥2 oligodendroglioma previously treated with lomustine as monotherapy or in combination
  • COHORT 2B2: Grade ≥2 oligodendroglioma previously treated with temozolomide as monotherapy or in combination
  • COHORT 3A: Grade ≥2 astrocytoma
  • COHORT 3B1: Grade ≥2 astrocytoma previously treated with lomustine as monotherapy or in combination
  • COHORT 3B2: Grade ≥2 astrocytoma previously treated with temozolomide as monotherapy or in combination
  • Measurable disease according to RANO 2.0 criteria.
  • Documented IDH1 and/or IDH2 gene mutations detected by immunochemistry or sequencing.
  • Karnofsky Performance Status (KPS) ≥ 70%.
  • Life expectancy ≥ 3 months.
  • Documented negative test for HIV-HBV-HCV. For HBV serology, the determination of HBsAg and anti-HBcAg Ab is required. In patients with serology documenting previous exposure to HBV, negative serum HBV-DNA is required. For HCV, HCV-RNA or HCV antibody test is required. Subjects with a positive test for HCV antibody but no detection of HCV-RNA indicating no current infection are eligible.
  • Female patients: female patients must be either documented not Women Of Childbearing Potential (WOCBP)\* or must have a negative pregnancy test within 14 days of starting treatment. Additionally WOCBP must agree to use, from the screening to 6 months following the last study drug administration, highly effective contraception methods, as defined by the "Recommendations for contraception and pregnancy testing in clinical trials" issued by the Head of Medicine Agencies' Clinical Trial Facilitation Group (www.hma.eu/ctfg.html) and which include, for instance, progesterone-only or combined (estrogen- and progesterone-containing) hormonal contraception associated with inhibition of ovulation, intrauterine devices, intrauterine hormone-releasing systems, bilateral tubal occlusion or vasectomized partner.
  • +3 more criteria

You may not qualify if:

  • Any therapy for recurrence/progression after alkylating chemotherapy, except resection.
  • Therapy for glioma within 4 weeks of start of study treatment.
  • Surgical resection of glioma within 4 weeks of start of study treatment.
  • Stereotactic biopsy of glioma within 2 weeks of start of study treatment.
  • Inability to undergo contrast-enhanced MRI.
  • Known history of allergy to TNF or lomustine or temozolomide, any excipient in the study medication or any other intravenously administered human proteins/peptides/antibodies.
  • Absolute neutrophil count (ANC) \< 1.5 x 10\^9/L; platelets \< 100 x 10\^9/L or hemoglobin (Hb) \< 9.0 g/dl.
  • Chronically impaired renal function as indicated by creatinine clearance \< 60 mL/min/1.73m2 or for patients older than 65 years without albuminuria or proteinuria, creatinine clearance \< 45 mL/min/1.73m2.
  • Inadequate liver function (ALT, AST, ALP ≥ 2.5 x ULN or total bilirubin ≥ 1.5 x ULN).
  • INR \> 1.5 ULN.
  • Any severe concomitant condition, which makes it undesirable for the patient to participate in the study or which could jeopardize compliance with the protocol, in the opinion of the investigator.
  • Active or history of autoimmune disease that might deteriorate when receiving an immuno-stimulatory agent, in the judgement of the investigator.
  • History within the last year of cerebrovascular disease and/or acute or subacute coronary syndromes including myocardial infarction, unstable or severe stable angina pectoris.
  • Heart insufficiency (\> Grade II, New York Heart Association (NYHA) criteria).
  • Clinically significant cardiac arrhythmias or requiring permanent medication.
  • +18 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

MeSH Terms

Conditions

Glioma

Interventions

TemozolomideLomustine

Condition Hierarchy (Ancestors)

Neoplasms, NeuroepithelialNeuroectodermal TumorsNeoplasms, Germ Cell and EmbryonalNeoplasms by Histologic TypeNeoplasmsNeoplasms, Glandular and EpithelialNeoplasms, Nerve Tissue

Intervention Hierarchy (Ancestors)

DacarbazineTriazenesOrganic ChemicalsImidazolesAzolesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsNitrosourea CompoundsUreaAmidesNitroso Compounds

Central Study Contacts

Teresa Hemmerle, PhD

CONTACT

Concetta Aulicino, Pharmaceutical Chemist

CONTACT

Study Design

Study Type
interventional
Phase
phase 2
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 30, 2025

First Posted

August 13, 2025

Study Start

January 30, 2026

Primary Completion (Estimated)

January 30, 2029

Study Completion (Estimated)

January 30, 2029

Last Updated

August 13, 2025

Record last verified: 2025-07

Data Sharing

IPD Sharing
Will not share